scholarly journals Using Lactic Acid Bacteria as Silage Inoculants or Direct-Fed Microbials to Improve In Vitro Degradability and Reduce Methane Emissions in Dairy Cows

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1482
Author(s):  
Nguyen Thi Huyen ◽  
Ines Martinez ◽  
Wilbert Pellikaan
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Ch4 Production ◽  
Silage Quality ◽  
Silage Inoculants ◽  
Silage Inoculant

The current study has two objectives: (1) To determine the effect of different lactic acid bacteria (LAB) strains’ inoculant on silage quality of fresh ryegrass (FR) and rain-treated ryegrass (RTR), and (2) to find the optimal way (silage inoculant vs. direct-fed microbial (DFM)) to use LAB strains in order to improve nutrient digestibility and reduce methane emission (CH4) in ruminant production. Five LAB strains were tested, Lactiplantibacillus plantarum AGR-1, L. plantarum AGR-2, Lactococcus lactis subsp. lactis biovar diacetylactis AGR-3, L. lactis subsp. lactis AGR-4 and L. lactis subsp. lactis AGR-5. Each LAB strain was inoculated at 106 cfu/g fresh weight into the FR and the RTR and ensiled for 60 days. After ensiling, the effect of LAB strains included as a DFM or silage inoculant on rumen digestibility and CH4 production were measured using an in vitro gas production system with three separate runs. The in vitro experiment consisted of 24 treatments (2 grasses (FR and RTR) × 2 ways (inoculant or DFM) × 6 strains (5 LAB strains + 1 Control)). The results indicated that the LAB strains’ inoculant treatments reduced (p < 0.0001) the dry matter (DM) losses, the NH3 concentration (p < 0.0001) and the pH (p = 0.0019) upon ensiling in both the FR and the RTR. The lowest values in dry matter (DM) loss and NH3 concentration were found in the L. plantarum (AGR-2) and L. lactis (AGR-5). The in vitro CH4 production was lower for silages inoculated with L. plantarum (AGR-1, p = 0.0054), L. lactis (AGR-4, p = 0.026), L. lactis (AGR-5, p = 0.029) and L. plantarum (AGR-2, p = 0.090), compared to the control. Methane production was lower (p = 0.0027) for LABs when used as silage inoculants, compared to being used as DFM. Lactic acid bacteria used as silage inoculants increased (p ≤ 0.0001) the in vitro DM and organic matter (OM) degradability both in the FR and the RTR, whereas LAB strains used as DFM showed no such effect. The DM and OM digestibility were highest in the L. plantarum (AGR-1, p = 0.0175). Among the five LAB strains used in the current study, L. plantarum (AGR-2) was the best candidate to improve silage quality. Our observations suggest that these LAB strains are most promising when used as silage inoculants and to be confirmed in vivo.

scholarly journals Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production

2021 ◽  
Vol 8 (6) ◽  
pp. 100
Author(s):  
Ehsan Oskoueian ◽  
Mohammad Faseleh Jahromi ◽  
Saeid Jafari ◽  
Majid Shakeri ◽  
Hieu Huu Le ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Rice Straw ◽  
Methane Production ◽  
Dry Matter ◽  
In Vitro Study ◽  
Microbial Populations ◽  
Different Types ◽  
Silage Quality

Bacterial inoculants are known to improve the quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB; L. plantarum, L. salivarius, L. reuteri, L. brevi, and S. bovis) inoculation (106 cfu/ DM) on rice straw silage quality and to determine these effects on ruminal fermentation characteristics, digestibility and microbial populations in an in vitro condition. Inoculated rice straw was ensiled for 15 and 30 days. For the in vitro study, rumen fluid was obtained from three rumen-fistulated bulls fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation with LAB improved (p < 0.05) the rice straw silage quality as indicated by higher dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB numbers, especially after 30 days of ensiling. Results from the in vitro study revealed that starting with the addition of LAB to rice straw silage improved in vitro fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility (p < 0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced, while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially for L. plantarum and S. bovis) improved silage quality, rumen fermentation parameters and microbial populations in vitro.

scholarly journals A lower cost method of preparing corn stover for Irpex lacteus treatment by ensiling with lactic acid bacteria

2020 ◽  
Vol 33 (8) ◽  
pp. 1273-1283
Author(s):  
Sasa Zuo ◽  
Di Jiang ◽  
Dongze Niu ◽  
Mingli Zheng ◽  
Ya Tao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Corn Stover ◽  
Lignin Content ◽  
Early Stage ◽  
Gas Production ◽  
Irpex Lacteus ◽  
Corn Stover Silage ◽  
Silage Quality

Objective: This study investigated a method of preparing corn stover for Irpex lacteus (<i>I. lacteus</i>) treatment to improve its <i>in vitro</i> rumen degradability under non-sterile conditions.Methods: Corn stover was inoculated with <i>Lactobacillus plantarum</i> (<i>L. plantarum</i>), <i>Lactobacillus buchneri</i> (<i>L. buchneri</i>), and an equal mixture of these strains, and ensiled for 0, 3, 7, 14, and 28 days. After each period, a portion of the silage was sampled to assess the silage quality, and another portion of the silage was further treated with <i>I. lacteus</i> at 28°C for 28 d. All the samples were analyzed for fermentation quality, chemical composition, and <i>in vitro</i> gas production (IVGP) as a measure of rumen fermentation capacity.Results: Lactic acid bacteria (LAB) was found to improve the silage quality of the corn stover, and the corn stover silage inoculated with <i>L. plantarum</i> produced more lactic acid and higher IVGP than other silage groups. The <i>I. lacteus</i> colonies flourished in the early stage of corn stover silage, especially on the 3-d corn stover silage inoculated with both <i>L. plantarum</i> and <i>L. buchneri.</i> This led to an 18% decrease in the acid detergent lignin content, and a 49.6% increase in IVGP compared with the raw stover.Conclusion: The combination of ensiling with the mixed LAB inoculation and <i>I. lacteus</i> treatment provided a cost-effective method for the improvement of the IVGP of corn stover from 164.8 mL/g organic matter (OM) to 246.6 mL/g OM.

scholarly journals Application of Different Types of Lactic Acid Bacteria Inoculant on Ensiled Rice Straw; Effects on Silage Quality, Rumen Fermentation, Methane Production and Microbial Population

2019 ◽  
Author(s):  
Ehsan Oskoueian ◽  
Saeid Jafari ◽  
Reza Noura ◽  
Mohammad Faseleh Jahromi ◽  
Goh Yong Meng ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Rice Straw ◽  
Methane Production ◽  
Dry Matter ◽  
Microbial Population ◽  
Microbial Populations ◽  
Different Types ◽  
Silage Quality

AbstractBacterial inoculants are known to improve quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB;L. plantarum,L. salivarius, L. reuteri, L. brevisandS. bovis) inoculation (106g−1DM) on rice straw silage quality and to examine these effects on ruminal fermentation characteristics, digestibility and microbial populations in anin vitrocondition. Inoculated rice straw was ensiled for 15 and 30 days. Forin vitrostudy, rumen liquor was obtained from two rumen fistulated mature cows fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation of LAB improved (P˂0.05) the rice straw silage quality such as increased dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB contents especially after 30 days of ensiling. Results fromin vitrostudy revealed that addition of LAB to the rice straw silage improved fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility (P˂0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially forL. plantarumandS. bovis) improved silage quality, rumen fermentation parameters and microbial populationsin vitro. However,in vivostudies need to confirm those effects.

scholarly journals Improving ensiling characteristics by adding lactic acid bacteria modifies in vitro digestibility and methane production of forage-sorghum mixture silage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chatchai Kaewpila ◽  
Pongsatorn Gunun ◽  
Piyawit Kesorn ◽  
Sayan Subepang ◽  
Suwit Thip-uten ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Nutritive Value ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Acid Fermentation ◽  
Design Factor ◽  
Forage Sorghum ◽  
Randomized Design

AbstractImproving the nutrition of livestock is an important aspect of global food production sustainability. This study verified whether lactic acid bacteria (LAB) inoculant could promote ensiling characteristics, nutritive value, and in vitro enteric methane (CH4) mitigation of forage sorghum (FS) mixture silage in attacking malnutrition in Zebu beef cattle. The FS at the soft dough stage, Cavalcade hay (CH), and cassava chip (CC) were obtained. The treatments were designed as a 4 × 2 factorial arrangement in a completely randomized design. Factor A was FS prepared without or with CH, CC, and CH + CC. Factor B was untreated or treated with Lactobacillus casei TH14. The results showed that all FS mixture silages preserved well with lower pH values below 4.0 and higher lactic acid contents above 56.4 g/kg dry matter (DM). Adding LAB boosted the lactic acid content of silages. After 24 h and 48 h of in vitro rumen incubation, the CC-treated silage increased in vitro DM digestibility (IVDMD) with increased total gas production and CH4 production. The LAB-treated silage increased IVDMD but decreased CH4 production. Thus, the addition of L. casei TH14 inoculant could improve lactic acid fermentation, in vitro digestibility, and CH4 mitigation in the FS mixture silages.

scholarly journals Growth performance, nutrient digestibility, and selected fecal microbiota are improved by β-glucan supplementation in weaner pigs

2018 ◽  
Vol 18 (3) ◽  
pp. 769-779
Author(s):  
Xin Jian Lei ◽  
Hyeok Min Yun ◽  
Yi Yang ◽  
In Ho Kim
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Growth Performance ◽  
Dry Matter ◽  
Basal Diet ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Coliform Bacteria ◽  
Weaner Pigs ◽  
Blood Profiles

Abstract This study was conducted to evaluate the effects of dietary β-glucan supplementation on growth performance, nutrient digestibility, blood profiles, and fecal characteristics in weaner pigs. A total of 140 weaner pigs [(Yorkshire × Landrace) × Duroc] with an average body weight of 6.37±1.14 kg were allotted to one of the following dietary treatments: 1) negative control (NC, basal diet); 2) positive control (PC, basal diet supplemented with 39 ppm Tiamulin); 3) basal diet supplemented with 0.1% β-glucan (G1); 4) basal diet supplemented with 0.2% β-glucan (G2). During days 22 to 42 and 1 to 42, pigs offered PC and G2 diets grew faster than those offered NC diet (P<0.05). Additionally, during the same periods, pigs fed G2 and PC diets exhibited improved gain:feed ratio compared to pigs receiving NC and G1 diets (P<0.05). Pigs fed G2 diet exhibited higher coefficient of total tract apparent digestibility of dry matter than those fed NC diet (P<0.05). There were no differences on blood profiles, fecal scores, fecal moisture, and fecal pH (P>0.05). Fecal lactic acid bacteria counts in G2 treatment were higher compared with PC and NC treatments (P<0.05). Coliform bacteria concentrations were decreased in pigs fed PC and G2 diets compared with those fed NC diet (P<0.05). In conclusion, the results of the current study indicate that dietary supplementation of 0.2% β-glucan can improve growth performance and dry matter digestibility, increase fecal lactic acid bacteria concentration but decrease fecal coliform bacteria concentration in weaner pigs.

The voluntary intake and digestibility by sheep of grass silages treated with different silage inoculants

1991 ◽  
Vol 1991 ◽  
pp. 82-82
Author(s):  
J A Rooke ◽  
F Kafilzadeh
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Live Weight ◽  
Random Order ◽  
Animal Performance ◽  
Silage Fermentation ◽  
Silage Inoculants ◽  
Silage Additives ◽  
Forage Harvester ◽  
Silage Inoculant

The selection of lactic acid bacteria for use as silage additives is normally based upon their ability to dominate the silage fermentation and not upon benefits in animal performance. The object of this study was to investigate whether two lactic acid bacteria selected for fermentation characteristics would support the same animal performance as an established silage inoculant.On 5 June 1989, first cut, predominantly perennial ryegrass (Lolium perenne) was ensiled direct with no wilting in plastolene silos of 2 tonne capacity. The herbage was harvested with a precision chop forage harvester and the following additive treatments were applied: None, control (C); Formic acid (Add-F, BP Nutrition, 850g/kg; 3 litres/tonne), (F); inoculant E. (Ecosyl, ICI Pic, 106Lactobacillus plantarum /g): inoculant A, (Pediococcus sp, 10 /g); inoculant B (L. plantarum 106 /g). The silages were fed to 6 wether sheep (Suffolk x Halfbred), initial live-weight, 40.1 kg (s.d. 2.22kg). Because the silages were unstable aerobically and restricted quantities of each silage were available, all sheep were fed the silages in the same (random) order.

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