Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production

Bacterial inoculants are known to improve the quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB; L. plantarum, L. salivarius, L. reuteri, L. brevi, and S. bovis) inoculation (106 cfu/ DM) on rice straw silage quality and to determine these effects on ruminal fermentation characteristics, digestibility and microbial populations in an in vitro condition. Inoculated rice straw was ensiled for 15 and 30 days. For the in vitro study, rumen fluid was obtained from three rumen-fistulated bulls fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation with LAB improved (p < 0.05) the rice straw silage quality as indicated by higher dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB numbers, especially after 30 days of ensiling. Results from the in vitro study revealed that starting with the addition of LAB to rice straw silage improved in vitro fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility (p < 0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced, while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially for L. plantarum and S. bovis) improved silage quality, rumen fermentation parameters and microbial populations in vitro.

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Application of Different Types of Lactic Acid Bacteria Inoculant on Ensiled Rice Straw; Effects on Silage Quality, Rumen Fermentation, Methane Production and Microbial Population

10.1101/612556 ◽

2019 ◽

Cited By ~ 1

Author(s):

Ehsan Oskoueian ◽

Saeid Jafari ◽

Reza Noura ◽

Mohammad Faseleh Jahromi ◽

Goh Yong Meng ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Rice Straw ◽

Methane Production ◽

Dry Matter ◽

Microbial Population ◽

Microbial Populations ◽

Different Types ◽

Silage Quality

AbstractBacterial inoculants are known to improve quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB;L. plantarum,L. salivarius, L. reuteri, L. brevisandS. bovis) inoculation (106g−1DM) on rice straw silage quality and to examine these effects on ruminal fermentation characteristics, digestibility and microbial populations in anin vitrocondition. Inoculated rice straw was ensiled for 15 and 30 days. Forin vitrostudy, rumen liquor was obtained from two rumen fistulated mature cows fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation of LAB improved (P˂0.05) the rice straw silage quality such as increased dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB contents especially after 30 days of ensiling. Results fromin vitrostudy revealed that addition of LAB to the rice straw silage improved fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility (P˂0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially forL. plantarumandS. bovis) improved silage quality, rumen fermentation parameters and microbial populationsin vitro. However,in vivostudies need to confirm those effects.

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Using Lactic Acid Bacteria as Silage Inoculants or Direct-Fed Microbials to Improve In Vitro Degradability and Reduce Methane Emissions in Dairy Cows

Agronomy ◽

10.3390/agronomy10101482 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1482

Author(s):

Nguyen Thi Huyen ◽

Ines Martinez ◽

Wilbert Pellikaan

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Dry Matter ◽

Gas Production ◽

Nutrient Digestibility ◽

Ch4 Production ◽

Silage Quality ◽

Silage Inoculants ◽

Silage Inoculant

The current study has two objectives: (1) To determine the effect of different lactic acid bacteria (LAB) strains’ inoculant on silage quality of fresh ryegrass (FR) and rain-treated ryegrass (RTR), and (2) to find the optimal way (silage inoculant vs. direct-fed microbial (DFM)) to use LAB strains in order to improve nutrient digestibility and reduce methane emission (CH4) in ruminant production. Five LAB strains were tested, Lactiplantibacillus plantarum AGR-1, L. plantarum AGR-2, Lactococcus lactis subsp. lactis biovar diacetylactis AGR-3, L. lactis subsp. lactis AGR-4 and L. lactis subsp. lactis AGR-5. Each LAB strain was inoculated at 106 cfu/g fresh weight into the FR and the RTR and ensiled for 60 days. After ensiling, the effect of LAB strains included as a DFM or silage inoculant on rumen digestibility and CH4 production were measured using an in vitro gas production system with three separate runs. The in vitro experiment consisted of 24 treatments (2 grasses (FR and RTR) × 2 ways (inoculant or DFM) × 6 strains (5 LAB strains + 1 Control)). The results indicated that the LAB strains’ inoculant treatments reduced (p < 0.0001) the dry matter (DM) losses, the NH3 concentration (p < 0.0001) and the pH (p = 0.0019) upon ensiling in both the FR and the RTR. The lowest values in dry matter (DM) loss and NH3 concentration were found in the L. plantarum (AGR-2) and L. lactis (AGR-5). The in vitro CH4 production was lower for silages inoculated with L. plantarum (AGR-1, p = 0.0054), L. lactis (AGR-4, p = 0.026), L. lactis (AGR-5, p = 0.029) and L. plantarum (AGR-2, p = 0.090), compared to the control. Methane production was lower (p = 0.0027) for LABs when used as silage inoculants, compared to being used as DFM. Lactic acid bacteria used as silage inoculants increased (p ≤ 0.0001) the in vitro DM and organic matter (OM) degradability both in the FR and the RTR, whereas LAB strains used as DFM showed no such effect. The DM and OM digestibility were highest in the L. plantarum (AGR-1, p = 0.0175). Among the five LAB strains used in the current study, L. plantarum (AGR-2) was the best candidate to improve silage quality. Our observations suggest that these LAB strains are most promising when used as silage inoculants and to be confirmed in vivo.

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Effect of Lactic Acid Bacteria on the Nutritive Value and In Vitro Ruminal Digestibility of Maize and Rice Straw Silage

Applied Sciences ◽

10.3390/app10217801 ◽

2020 ◽

Vol 10 (21) ◽

pp. 7801

Author(s):

Tabita Dameria Marbun ◽

Kihwan Lee ◽

Jaeyong Song ◽

Chan Ho Kwon ◽

Duhak Yoon ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Rice Straw ◽

Dry Matter ◽

Nutritive Value ◽

Positive Control ◽

Negative Control ◽

The Other ◽

Silage Additives

A study was conducted to determine the effects of lactic acid bacteria (LAB) on nutritive value and in vitro rumen digestibility of maize and rice straw silages. Two identical experiments were carried out for each of the two silages. A total of five treatments were used for each experiment: (1) negative control (NC); (2) positive control (PC); (3) Lactobacillus plantarum (LPL); (4) L. paracasei (LPA); and (5) L. acidophilus (LA). Each treatment was then divided into four ensiling periods: 3, 7, 20, and 40 days with three replications. The LPL treatment had significantly higher dry matter (DM), lower ammonia-N, and a lower number of fungi on maize silage after 40 days (p < 0.05). On the other hand, the LA treatment increased DM and CP content, reduced NDF and ADF contents compared to NC, and also produced more lactic acid compared to the other LAB-treated rice straw silages. Results of the in vitro rumen fermentation of maize silages showed no significant differences in DMD after LAB inoculation. However, higher DMD and ruminal ammonia-N were shown by rice straw ensiled with L. acidophilus. In conclusion, silage additives, which could improve the ensiling process of maize and rice straw, appeared to be different and substrate specific.

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