scholarly journals Overview of the Classical Histone Deacetylase Enzymes and Histone Deacetylase Inhibitors

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Katherine Ververis ◽  
Tom C. Karagiannis
Keyword(s):  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Cellular Proliferation ◽  
Cyclic Peptide ◽  
Cell Lymphoma ◽  
Trichostatin A ◽  
Therapeutic Modalities ◽  
Solid Malignancies ◽  
The Us

The important role of histone deacetylase enzymes in regulating gene expression, cellular proliferation, and survival has made them attractive targets for the development of histone deacetylase inhibitors as anticancer drugs. Suberoylanilide hydroxamic acid (Vorinostat, Zolinza), a structural analogue of the prototypical Trichostatin A, was approved by the US Food and Drug Administration for the treatment of advanced cutaneous T-cell lymphoma in 2006. This was followed by approval of the cyclic peptide, depsipeptide (Romidepsin, Istodax) for the same disease in 2009. Currently numerous histone deacetylase inhibitors are undergoing preclinical and clinical trials for the treatment of hematological and solid malignancies. Most of these studies are focused on combinations of histone deacetylase inhibitors with other therapeutic modalities, particularly conventional chemotherapeutics and radiotherapy. The aim of this paper is to provide an overview of the classical histone deacetylase enzymes and histone deacetylase inhibitors with an emphasis on potential combination therapies.

scholarly journals Role of Hydroxamate-Based Histone Deacetylase Inhibitors (Hb-HDACIs) in the Treatment of Solid Malignancies

Cancers ◽  
2013 ◽  
Vol 5 (4) ◽  
pp. 919-942 ◽  
Author(s):  
Antonino Grassadonia ◽  
Pasquale Cioffi ◽  
Felice Simiele ◽  
Laura Iezzi ◽  
Marinella Zilli ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Solid Malignancies

scholarly journals Histone deacetylase inhibitors Induce Expression of Chromosomally Tagged Variant-Specific Surface Protein Genes in Giardia lamblia

2020 ◽  
Author(s):  
Daniel Roberto Orozco ◽  
Srinivas Garlapati
Keyword(s):  
Histone Deacetylase ◽  
Giardia Lamblia ◽  
Histone Deacetylase Inhibitors ◽  
Trichostatin A ◽  
Surface Protein ◽  
Rt Pcr ◽  
Coding Sequences ◽  
Sequence Encoding ◽  
Gene Switching

Abstract Objective RNA interference and miRNA mediated mechanisms have been proposed to explain the expression of a specific variant of VSP at a time on the surface of Giardia lamblia. Recently, epigenetic mechanisms involving histone acetylations have been proposed to explain the process of antigenic switching in Giardia lamblia. However, due to the limited availability of specific antibodies for all the vsp variants present in the genome, it was difficult to monitor vsp gene switching. In this study, we have used an endogenous tagging method to tag specific vsp genes vsp1267 and vsp9B10A with a sequence encoding hemagglutinin (HA) epitope at the 3’end of the coding sequences without altering the 5’ upstream elements. With this method, we have monitored the expression of the tagged vsp genes in cells treated with histone deacetylase inhibitors using RT-PCR. Results Our results show that vsp1267-3XHA can be induced by treatment with sodium 4-phenylbutyrate, M344 and splitomicin but not by apicidin and Trichostatin A, while vsp9B10A-3XHA expression can be induced by Trichostatin A and splitomicin but not by sodium 4-phenylbutyrate, M344 and apicidin. The induced expression of these variants was not due to growth inhibition. These results support the role of histone acetylations in vsp expression.

scholarly journals Histone Deacetylase Inhibitors Increase the Embryogenic Potential and Alter the Expression of Embryogenesis-Related and HDAC-Encoding Genes in Grapevine (Vitis vinifera L., cv. Mencía)

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1164
Author(s):  
Óscar Martínez ◽  
Verónica Arjones ◽  
María Victoria González ◽  
Manuel Rey
Keyword(s):  
Vitis Vinifera ◽  
Histone Deacetylase ◽  
Sodium Butyrate ◽  
Somatic Embryos ◽  
Histone Deacetylase Inhibitors ◽  
Trichostatin A ◽  
Vitis Vinifera L ◽  
Molecular Response ◽  
Embryogenic Competence ◽  
Encoding Genes

The low induction rates of somatic embryogenesis are one of the main limitations in its routine application in the grapevine (Vitis vinifera L.). The use of an induction medium containing histone deacetylase inhibitors (trichostatin A and, mainly, sodium butyrate) resulted in an improvement of the embryogenic responses in grapevine (cv. Mencía) cotyledonary and recently germinated somatic embryos. The relative expression of several grapevine genes related to embryogenic competence or encoding histone deacetylase enzymes was studied in cotyledonary somatic embryos that were cultured in the presence of 0.5 mM sodium butyrate. The results showed a significant overexpression of the BBM and VvSERK2 genes after 24 h of culture, whereas the VvWOX2 gene was underexpressed less in treated versus untreated explants. The results suggest that the inhibitor may trigger a molecular response related to an increase in embryogenic competence and changes in the expression of associated genes. The treatment with sodium butyrate also produced significant variations in the expression of several histone deacetylase enzyme-encoding genes. These results may enhance the possibility of obtaining somatic embryos, reducing the seasonal constraints associated with the use of floral explants in grapevines.

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