Selenium increases cell proliferation after citrate treatment on L929 fibroblast cell line

2021 ◽  
Author(s):  
Ahu Soyocak ◽  
Dilek Duzgun Ergun
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
L929 Fibroblast

Toxicity evaluation of ZnO nanostructures on L929 fibroblast cell line using MTS assay

2015 ◽  
Author(s):  
Siti Khadijah Mohd Bakhori ◽  
Shahrom Mahmud ◽  
Ling Chuo Ann ◽  
Azman Seeni Mohamed ◽  
Siti Nazmin Saifuddin ◽  
...  
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Zno Nanostructures ◽  
Toxicity Evaluation ◽  
L929 Fibroblast ◽  
Mts Assay

scholarly journals Momordica and Pycnogenol Can Tolerate Imazamox Induced Toxicity in L929 Cells Line: In Vitro Study

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1584
Author(s):  
Ali Taghizadehghalehjoughi ◽  
Betul Cicek
Keyword(s):  
Protective Effect ◽  
Cell Line ◽  
Low Dose ◽  
Antioxidant Activities ◽  
Fibroblast Cell ◽  
High Dose ◽  
Protective Effects ◽  
Fibroblast Cell Line ◽  
Cell Viability Assay ◽  
L929 Fibroblast

Undesirable side effects that result from the random use of herbicides in developing countries are widespread. Imazamox is a widely used herbicide and has toxic effect on humans. Momordica charantia has been reported to possess many benefits and medicinal properties. Pycnogenol (PYC) is a natural plant extract from the bark of Pinus pinaster Aiton and has potent antioxidant activities. The aim of this study was to evaluate the protective effects of pycnogenol and momardica on induced imazamox toxicity effects on L929 fibroblast cell line. L929 fibroblast cells were cultured in the appropriate culture medium. Toxic concentration of imazamox 250 μM, were administered 30 min prior to momordica and pycnogenol (10−1–10−5 concentration) on L929 fibroblast cell line for 24 h. The cell viability assay was determined by using MTT test. TAC-TOS analysis were used to evaluate antioxidant and oxidant status. According to our study pycnogenol high dose showned protective effect whereas momordica low dose showned protective effect p < 0.05. In current study pycnogenol increased TAC capacity in high dose but in lower dose pycnogenol did not show any promise (p < 0.05). Momordica results showed correlation with MTT result. According to this analyse momordica only in low dose increased anti oxidant capacity and in addition, only in low dose TOS level were decreased (p > 0.05). In conclusion momordica and pycnogenol showed promise to reduced imazamox toxicity.

Replicative senescence, telomere shortening and cell proliferation rate in Gaddi goat's skin fibroblast cell line

2007 ◽  
Vol 31 (10) ◽  
pp. 1257-1264 ◽  
Author(s):  
Neelam Gupta ◽  
Ritu Taneja ◽  
Alok Pandey ◽  
Manishi Mukesh ◽  
Hari Singh ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Skin Fibroblast ◽  
Replicative Senescence ◽  
Fibroblast Cell ◽  
Proliferation Rate ◽  
Fibroblast Cell Line ◽  
Telomere Shortening ◽  
Cell Proliferation Rate

scholarly journals Cytotoxicity evaluation of a new fast set highly viscous conventional glass ionomer cement with L929 fibroblast cell line

2011 ◽  
Vol 14 (4) ◽  
pp. 406 ◽  
Author(s):  
Hany Mohamed Aly Ahmed ◽  
Norhayati Luddin ◽  
Deepti Saini ◽  
NorShamsuria Omar ◽  
Rajan Saini
Keyword(s):  
Cell Line ◽  
Glass Ionomer Cement ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Glass Ionomer ◽  
L929 Fibroblast ◽  
Ionomer Cement

Liposomal formulation of chondroitin sulfate enhances its antioxidant and anti-inflammatory potential in L929 fibroblast cell line

2013 ◽  
Vol 23 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Oana Craciunescu ◽  
Lucia Moldovan ◽  
Magdalena Moisei ◽  
Mihaela Trif
Keyword(s):  
Chondroitin Sulfate ◽  
Cell Line ◽  
Fibroblast Cell ◽  
Liposomal Formulation ◽  
Fibroblast Cell Line ◽  
L929 Fibroblast ◽  
Anti Inflammatory

scholarly journals Transcriptional regulation of two genes specifically induced by glucose starvation in a hamster mutant fibroblast cell line.

1983 ◽  
Vol 258 (1) ◽  
pp. 597-603 ◽  
Author(s):  
A S Lee ◽  
A M Delegeane ◽  
V Baker ◽  
P C Chow
Keyword(s):  
Transcriptional Regulation ◽  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Glucose Starvation

Evidence of an oscillating peripheral clock in an equine fibroblast cell line and adipose tissue but not in peripheral blood

2006 ◽  
Vol 192 (7) ◽  
pp. 743-751 ◽  
Author(s):  
Barbara A. Murphy ◽  
Mandi M. Vick ◽  
Dawn R. Sessions ◽  
R. Frank Cook ◽  
Barry P. Fitzgerald
Keyword(s):  
Adipose Tissue ◽  
Cell Line ◽  
Peripheral Blood ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Peripheral Clock

Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

Biologia ◽  
2008 ◽  
Vol 63 (2) ◽  
Author(s):  
Marica Theiszová ◽  
Soňa Jantová ◽  
Silvia Letašiová ◽  
Ľuboš Valík ◽  
Martin Palou
Keyword(s):  
Comparative Study ◽  
Cell Line ◽  
Dna Content ◽  
Fibroblast Cell ◽  
Cell Number ◽  
Fibroblast Cell Line ◽  
3T3 Cells ◽  
End Points ◽  
Basal Cytotoxicity ◽  
Nih 3T3

AbstractThe number of biomaterials used in biomedical applications has rapidly increased in the past two decades. Fluorapatite (FA) is one of the inorganic constituents of bone or teeth used for hard tissue repairs and replacements. Fluor-hydroxyapatite (FHA) is a new synthetically prepared composite that in its structure contains the same molecular concentration of OH− groups and F− ions. The aim of this experimental investigation was to use the embryonal mouse fibroblast cell line NIH-3T3 for comparative study of basal cytotoxicity of fluoridated biomaterials FHA and FA discs. Hydroxyapatite (HA) disc, high-density polyethylene as negative control and polyvinyl chloride (PVC) containing organotin stabilizer as positive control were used as standard biomaterials. The appropriateness of the use of NIH-3T3 cells and their sensitivity for tested biomaterials were evaluated on the basis of five cytotoxic end points: cell proliferation, cell morphology, lactate dehydrogenase (LDH) released, protein and DNA cell content. The basal cytotoxicity of FHA, FA and HA discs was measured by direct contact method. FHA composite, FA and HA demonstrated in cell line NIH-3T3 nearly similar basal cytotoxicity increasing with the time of treatment. After 72 h of biomaterials treatment, about 25% inhibition of cell number, unchanged morphology of dividing cells, 6.31–0.16% increase of released LDH, about 10% inhibition of cell protein content and about 20% inhibition of DNA content was found. On the other hand, from the growth rates it resulted that NIH-3T3 cells, affected by tested biomaterials, divided about 20% slowlier than the control (untreated cells). Using the linear regression analysis we found out that deviations in measurements of cytotoxicity by four methods were as follows: less than 10% for cell number, protein and DNA content methods and 12.4% for released LDH method. Based on a good correlation of the cytotoxicity of biomaterials obtained from all end points we could conclude that fibroblast NIH-3T3 cell line was appropriate for measuring the basal cytoxicity of tested biomaterials.

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