scholarly journals Cytotoxicity evaluation of a new fast set highly viscous conventional glass ionomer cement with L929 fibroblast cell line

2011 ◽  
Vol 14 (4) ◽  
pp. 406 ◽  
Author(s):  
Hany Mohamed Aly Ahmed ◽  
Norhayati Luddin ◽  
Deepti Saini ◽  
NorShamsuria Omar ◽  
Rajan Saini
Keyword(s):  
Cell Line ◽  
Glass Ionomer Cement ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Glass Ionomer ◽  
L929 Fibroblast ◽  
Ionomer Cement

Toxicity evaluation of ZnO nanostructures on L929 fibroblast cell line using MTS assay

2015 ◽  
Author(s):  
Siti Khadijah Mohd Bakhori ◽  
Shahrom Mahmud ◽  
Ling Chuo Ann ◽  
Azman Seeni Mohamed ◽  
Siti Nazmin Saifuddin ◽  
...  
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Zno Nanostructures ◽  
Toxicity Evaluation ◽  
L929 Fibroblast ◽  
Mts Assay

scholarly journals Momordica and Pycnogenol Can Tolerate Imazamox Induced Toxicity in L929 Cells Line: In Vitro Study

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1584
Author(s):  
Ali Taghizadehghalehjoughi ◽  
Betul Cicek
Keyword(s):  
Protective Effect ◽  
Cell Line ◽  
Low Dose ◽  
Antioxidant Activities ◽  
Fibroblast Cell ◽  
High Dose ◽  
Protective Effects ◽  
Fibroblast Cell Line ◽  
Cell Viability Assay ◽  
L929 Fibroblast

Undesirable side effects that result from the random use of herbicides in developing countries are widespread. Imazamox is a widely used herbicide and has toxic effect on humans. Momordica charantia has been reported to possess many benefits and medicinal properties. Pycnogenol (PYC) is a natural plant extract from the bark of Pinus pinaster Aiton and has potent antioxidant activities. The aim of this study was to evaluate the protective effects of pycnogenol and momardica on induced imazamox toxicity effects on L929 fibroblast cell line. L929 fibroblast cells were cultured in the appropriate culture medium. Toxic concentration of imazamox 250 μM, were administered 30 min prior to momordica and pycnogenol (10−1–10−5 concentration) on L929 fibroblast cell line for 24 h. The cell viability assay was determined by using MTT test. TAC-TOS analysis were used to evaluate antioxidant and oxidant status. According to our study pycnogenol high dose showned protective effect whereas momordica low dose showned protective effect p < 0.05. In current study pycnogenol increased TAC capacity in high dose but in lower dose pycnogenol did not show any promise (p < 0.05). Momordica results showed correlation with MTT result. According to this analyse momordica only in low dose increased anti oxidant capacity and in addition, only in low dose TOS level were decreased (p > 0.05). In conclusion momordica and pycnogenol showed promise to reduced imazamox toxicity.

In vitro Biocompatibility Tests of Glass Ionomer Cements Impregnated with Collagen or Bioactive glass to Fibroblasts

2012 ◽  
Vol 36 (3) ◽  
pp. 269-274 ◽  
Author(s):  
C Subbarao ◽  
P Neelakantan ◽  
CV Subbarao
Keyword(s):  
Bioactive Glass ◽  
Cell Attachment ◽  
Glass Ionomer Cement ◽  
Mineral Trioxide Aggregate ◽  
Fibroblast Cell ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Electron Microscopes ◽  
Ionomer Cement

Aim and Design: To evaluate the biocompatibility of glass ionomer cement (GIC) impregnated with collagen or bioactive glass to BHK-21 fibroblasts in vitro. Mineral Trioxide Aggregate was used as the standard for comparison. Human maxillary central incisors (n=70) were instrumented with a rotary NiTi system and filled. Following resection of the apical 3mm, root end cavities were prepared and restored with conventional GIC (group 1) or GIC with 0.01%, 0.1% or 1% collagen (groups 2, 3, 4 respectively) or, 10%, 30 % or 50% bioactive glass (groups 5,6,7 respectively), or Mineral Trioxide Aggregate (group 8). The root slices were incubated in tissue culture plates with BHK-21 fibroblast cell line. Phase contrast and scanning electron microscopes were used to score cell quantity, morphology and cell attachment. The data were statistically analyzed by one way ANOVA with Post Hoc Tukey HSD test (p = 0.05). Results and conclusions: Group 5 showed the highest scores which was significantly higher than all other groups (p&lt;0.05) except group 8, with which there was no significant difference (p&gt;0.05). Glass ionomer cement with 10% bioactive glass showed better adhesion and spreading of cells than glass ionomer cement with 0.01% collagen. The biocompatibility of collagen and bioactive glass was concentration dependent. The addition of bio active glass improved the biocompatibility of glass ionomer cement to fibroblasts better than addition of collagen.

Microleakage of class II restoration with zirconia inlay: An in vitro study

2019 ◽  
pp. 61-67
Author(s):  
Xuan Anh Ngoc Ho ◽  
Anh Chi Phan ◽  
Toai Nguyen
Keyword(s):  
Glass Ionomer Cement ◽  
Class Ii ◽  
Resin Cement ◽  
Glass Ionomer ◽  
Resin Modified Glass Ionomer ◽  
Group 2 ◽  
Self Etch ◽  
Ionomer Cement ◽  
Group 1 ◽  
Composite Restoration

Background: Class II restoration with zirconia inlay is concerned by numerous studies about the luting coupling between zirconia inlay and teeth. The present study was performed to evaluate the microleakage of Class II zirconia inlayusing two different luting agents and compare to direct restoration using bulk fill composite. Aims: To evaluate the microleakage of Class II restorations using three different techniques. Materials and methods: The study was performed in laboratory with three groups. Each of thirty extracted human teeth was prepared a class II cavity with the same dimensions, then these teeth were randomly divided into 3 groups restored by 3 different approaches. Group 1: zirconia inlay cemented with self-etch resin cement (Multilink N); Group 2: zirconia inlay cemented with resin-modified glass ionomer cement (Fuji Plus); Group 3: direct composite restoration using bulk fill composite(Tetric N-Ceram Bulk Fill). All restorations were subjected to thermal cycling (100 cycles 50C – 55 0C), then immersed to 2% methylene blue solution for 24 hours. The microleakage determined by the extent of dye penetration along the gingival wall was assessed using two methods: quantitative and semi-quantitative method. Results: Among three types of restorations, group 1 demonstrated the significantly lower rate of leakage compared to the others, while group 2 and 3 showed no significant difference. Conclusion: Zirconia inlay restoration cemented with self-etch resin cement has least microleakage degree when compare to class II zirconia inlay restoration cemented with resin-modified glass ionomer cement and direct composite restoration using bulk fill composite. Key words: inlay, zirconia ceramic, class II restoration, microleakage.

Comparative Evaluation of Mechanical Properties of Ceramic Reinforced Glass Ionomer Cement and Type IX GIC:An Invitro Study

2019 ◽  
Vol 10 (12) ◽  
pp. 35
Author(s):  
Nagalakshmi Chowdhary ◽  
N. K. Kiran ◽  
A. Lakshmi Priya ◽  
Rajashekar Reddy ◽  
Arvind Sridhara ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Comparative Evaluation ◽  
Glass Ionomer Cement ◽  
Glass Ionomer ◽  
Ionomer Cement
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