scholarly journals Identification of Somatic Antigens of Adult Fasciola gigantica Isolated from Bali Cattle

2021 ◽  
Vol 11 (1) ◽  
pp. 138-145
Author(s):  
Made Sriasih ◽  
Ahmad Munjizun
Keyword(s):  
Gel Electrophoresis ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Fasciola Gigantica ◽  
Molecular Weights ◽  
Bos Javanicus ◽  
Antigenic Proteins ◽  
Bali Cattle ◽  
Liver Flukes ◽  
Somatic Extract

In most tropical countries, such as Indonesia, fasciolosis is generally caused by Fasciola gigantica known as tropical liver fluke. However, most fasciolosis serodiagnostic tests have been developed solely for diagnosing fasciolosis caused by Fasciola hepatica (non-tropical liver fluke), and very few have been specifically designed for F. gigantica. The aim of this study was to determine the profile of antigenic proteins from the somatic extract of F. gigantica isolated from Bali cattle (Bos javanicus). The liver flukes were collected from a slaughtering house in Mataram, Indonesia. The somatic extracts were prepared by homogenizing in buffers containing 0.05 M NaCl, 0.02 M PMSF, and 0.05% Triton X-100. The characterization of the somatic extract proteins was performed using one-dimension gel electrophoresis and followed by Western blotting to determine the profile of its antigenic proteins. There were 14 bands of the somatic extracts with an estimated molecular weight ranging from 8 to105 8 kDa shown on the gel electrophoresis. The results of the Western blot show that there were five prominent protein bands. Three out of five prominent antigenic proteins with molecular weights of 8, 27, and 33 kDa are promising to enrich the existence of antigens that have immunodiagnostic value for fasciolosis. Therefore, further studies are required to examine more deeply the potency of those three antigenic somatic proteins of F. gigantica.

scholarly journals Adenomatous Cholecystitis in Cattle with Chronic Fascioliasis

1974 ◽  
Vol 11 (5) ◽  
pp. 407-416 ◽  
Author(s):  
A. H. Cheema
Keyword(s):  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Inflammatory Cells ◽  
Fasciola Gigantica ◽  
Clinical Effects ◽  
Fibrous Stroma ◽  
Submucosal Glands ◽  
Glandular Structures ◽  
Mitotic Figures ◽  
Liver Flukes

Gall bladders from 80 mature cattle infested with liver flukes were shrunken; their walls were thickened; and mucosa was raised into rugose folds, nodules and papillary projections. All contained thick bile and mature liver flukes, predominantly Fasciola gigantica and to a lesser extent Fasciola hepatica. There was an adenomatous proliferation of the lining epithelium as well as of submucosal glands forming numerous tubuloalveolar structures and cysts lined with a uniform layer of columnar epithelium. Fibrous stroma was more than normal, and there was a mild to heavy infiltration by inflammatory cells. Some bladders had many enterochromaffin cells in the epithelium lining the glandular structures. Mitotic figures were rare, and there was no evidence of malignancy. The condition is inflammatory and hyperplastic and is believed to be common in cattle infested with liver fluke. The clinical effects of this condition have not been studied.

Molecular evidence shows that the liver fluke Fasciola gigantica is the predominant Fasciola species in ruminants from Pakistan

2015 ◽  
Vol 90 (2) ◽  
pp. 206-213 ◽  
Author(s):  
U. Chaudhry ◽  
B. van Paridon ◽  
M.Z. Shabbir ◽  
M. Shafee ◽  
K. Ashraf ◽  
...  
Keyword(s):  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Fasciola Gigantica ◽  
Molecular Evidence ◽  
Human Populations ◽  
Rdna Its ◽  
Egg Morphology ◽  
Specialist Knowledge ◽  
Multiple Species ◽  
Liver Flukes

AbstractFascioliasis is an important disease affecting livestock, with great costs to producers worldwide. It has also become a serious issue for human populations in some endemic areas as an emerging zoonotic infection. There are two Fasciola species of liver fluke responsible for this disease, which occur worldwide, Fasciola hepatica and Fasciola gigantica. Identifying these two species on the basis of adult or egg morphology requires specialist knowledge due to the similarity of characters, and may misidentify putative intermediate or hybrid forms. In this study we sequenced the internal transcribed spacer 2 (ITS-2) rDNA of liver flukes collected from multiple species of hosts from seven localities in the Punjab and Baluchistan provinces of Pakistan, to determine the distribution of these two species. All 46 flukes processed in this study, collected from seven sites, showed the rDNA ITS-2 genotype corresponding to F. gigantica, contradicting previous reports, based on adult and egg morphology, that both species are present in Pakistan, with F. hepatica being the more common.

scholarly journals Morphometry of Liver Fluke (Fasciola Gigantica) Infecting Balinese Cattle

2019 ◽  
Vol 2 (1) ◽  
pp. 10
Author(s):  
Putu Anna Oktaviana ◽  
Nyoman Adi Suratma ◽  
I Nengah Wandia
Keyword(s):  
Body Length ◽  
Morphometric Analysis ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Descriptive Study ◽  
Fasciola Gigantica ◽  
Body Width ◽  
Bali Cattle ◽  
Tropical Climates ◽  
Temperate Climates

Fasciola hepatica and Fasciola gigantica belong to Trematoda family which can cause fasciolosis. Fasciola hepatica is commonly found in temperate climates, while Fasciola gigantica is found in areas with wet tropical climates. Research on Fasciola gigantica especially in Bali is still very little, was just limited to the prevalence, epidemiology and histopathology of liver. This study aims to determine the morphometry of Fasciola gigantica infecting Bali cattle in Bali. This descriptive study used 43 samples of worms. Measurement of morphometry is done by microscope equipped with camera Axio Cam ERc 5S and software used is IMAGEJ and ZEN LITE. The morphometric analysis showed that there were two differentiating factors that is PB (Body Length) and LB (Body Width). The morphometric of Fasciola gigantica in Bali is smaller compared to Fasciola gigantica in general but larger than Fasciola hepatica.

Characterisation of two calmodulin-like proteins from the liver fluke, Fasciola hepatica

2007 ◽  
Vol 388 (6) ◽  
pp. 593-599 ◽  
Author(s):  
Sean L. Russell ◽  
Neil V. McFerran ◽  
Elizabeth M. Hoey ◽  
Alan Trudgett ◽  
David J. Timson
Keyword(s):  
Metal Binding ◽  
Metal Ion ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Expression Vectors ◽  
Metal Ion Binding ◽  
Ion Sensing ◽  
Calcium Magnesium ◽  
Ion Binding Sites ◽  
Liver Flukes

Abstract Calmodulin is a calcium ion-sensing signalling protein found in eukaryotics. Two calmodulin-like gene sequences were identified in an EST library from adult liver flukes. One codes for a protein (FhCaM1) homologous to mammalian calmodulins (98% identity), whereas the other protein (FhCaM2) has only 41% identity. These genes were cloned into expression vectors and the recombinant proteins were expressed in Escherichia coli. Gel shift assays showed that both proteins bind to calcium, magnesium and zinc ions. Homology models have been built for both proteins. As expected, FhCaM1 has a highly similar structure to other calmodulins. Although FhCaM2 has a similar fold, its surface charge is higher than FhCaM1. One of the potential metal ion-binding sites has lower metal-ion co-ordination capability, while another has an adjacent lysine residue, both of which may decrease the metal-binding affinity. These differences may reflect a specialised role for FhCaM2 in the liver fluke.

Innate and adaptive resistance of Indonesian Thin Tail sheep to liver fluke: A comparative analysis of Fasciola gigantica and Fasciola hepatica infection

2011 ◽  
Vol 178 (3-4) ◽  
pp. 264-272 ◽  
Author(s):  
Jill Pleasance ◽  
Herman W. Raadsma ◽  
S.E. Estuningsih ◽  
S. Widjajanti ◽  
Els Meeusen ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Fasciola Gigantica ◽  
Hepatica Infection ◽  
Adaptive Resistance

scholarly journals Helminth infection-induced carcinogenesis: spectrometric insights from the liver flukes, Opisthorchis and Fasciola

2019 ◽  
Author(s):  
Maria João Gouveia ◽  
Maria Y. Pakharukova ◽  
Banchob Sripa ◽  
Gabriel Rinaldi ◽  
Paul J. Brindley ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Liver Fluke ◽  
Helminth Infection ◽  
Fasciola Hepatica ◽  
Clonorchis Sinensis ◽  
Opisthorchis Viverrini ◽  
International Agency ◽  
Fresh Water Fish ◽  
Liver Flukes ◽  
Group 1

AbstractChronic infections with the flatworm parasites Opisthorchis viverrini, Clonorchis sinensis and Schistosoma haematobium are classified as group 1 biological carcinogens, i.e. definitive causes of cancer. In addition, we reported findings that support the inclusion of Opisthorchis felineus in this list of biological carcinogens. By contrast, infections with close phylogenetic relatives including Fasciola hepatica have not been associated with carcinogenesis. Earlier reports revealed of oxysterol metabolites of Opisthorchis liver fluke origin conjugated with DNA bases, suggesting that the generation of these DNA-adducts may underlie the mutagenicity and carcinogenicity of the infection with these food-borne pathogens. Here we employed liquid chromatography-mass spectrometry (LC-MS/MS) to investigate, compare and contrast spectrograms of soluble extracts from F. hepatica adult worms from bile ducts of cattle with those from O. viverrini and O. felineus from experimentally-infected hamsters. F. hepatica displayed a complex spectrophotometric profile. F. hepatica and Opisthorchis spp. shared several common compounds including oxysterol-like metabolites, bile acids and DNA-adducts, but the spectrometric profiles of these Opisthorchis species included far fewer compounds than F. hepatica. These findings support the postulate that oxysterol-like metabolites of parasite origin can initiate carcinogenesis and they point to a molecular basis for the inconsistencies among major groups of liver flukes concerning infection-induced malignancy.Author SummarySeveral species of trematodes are parasites of the human hepatobiliary tract. Infection with two of these flukes, Clonorchis sinsensis and Opisthorchis viverrini, fresh water fish-borne parasites that occur in East Asia is classified as group 1 carcinogens by the International Agency for Research on Cancer (IARC), i.e. definitive causes of cancer in humans. By contrast, infection with a different liver fluke, Fasciola hepatica, does not lead to malignant transformation of the biliary tract. Given the close phylogeny of all three parasites, this difference in carcinogenicity is intriguing and, if explained, likely of value in novel therapeutic approaches. The importance of the current findings is informative because they present a mass spectrometric analysis and catalog of the similarities and differences between fluke of the genus Opisthorchis and F. hepatica, potentially identifying carcinogenic metabolites of liver fluke origin. These metabolites can be expected to provide deeper understanding of helminth infection induced malignancy.

scholarly journals PATHOLOGICAL ALTERATION OF BALI CATTLE HEPATOBILIARY SYSTEM INFECTED WITH FASCIOLA GIGANTICA

2020 ◽  
Vol 21 (4) ◽  
pp. 654-661
Author(s):  
Ida Bagus Oka Winaya ◽  
Anak Agung Ayu Mirah Adi ◽  
I Ketut Berata ◽  
I Made Kardena ◽  
Ida Bagus Windia Adnyana ◽  
...  
Keyword(s):  
Bile Ducts ◽  
Liver Fluke ◽  
Mononuclear Cells ◽  
Neutrophil Infiltration ◽  
Economic Loss ◽  
Fasciola Gigantica ◽  
Fixed Tissue ◽  
Bile Duct Wall ◽  
Hepatobiliary System ◽  
Bali Cattle

Liver fluke infections is an important parasitic disease that common attack cattle, buffalo and others ruminant. The disease in the tropics is caused by Fasciola gigantica trematodes. Fasciolosis found in Bali cattle besides causing high economic loss also has the opportunity infect humans. This study aims to determine the pathology changes of Bali cattle hepatobiliary system infected by F. gigantica. In January 2019 were examined a hundred of liver tissue Bali cattle. Based on the sex as many as 75 bali cattle are cows and 25 are bulls. On macroscopic examination was found fifteen bali cattles in gallbladder containing of F. gigantica. In positive Fasciolosis the surface of liver look uneven with enlargement of lymph nodes. The tissue of Bali cattle liver infected with F. gigantica is cut into 1x1x1 cm and put in a pot that has been filled with 10% neutral buffered formaline. The fixed tissue then processed in a tissue processor and stained with hematoxylin-eosin (HE). Microscopic examination was found bile ducts proliferation and blocked of the lumen accompanied by cholangio cirrhosis. Found longitudinal pieces of Fasciola gigantica liver fluke surrounded by fibrous connective tissue and multifocal coagulative necrosis with fibroblast proliferation. In some location the infiltration of neutrophil cells around the bile ducts can also be seen. Congestion, bleeding, and neutrophil infiltration are also seen in areas of necrosis. In the bile duct wall bleeding, adenomatus hyperplasia, infiltration of mononuclear cells with mild intensity and foci of calcification are found. It can be concluded that there was a change with severe intensity accompanied by chronic inflammation in the hepatobiliary system of Bali cattle with Fasciolosis.

scholarly journals Helminth infection-induced carcinogenesis: spectrometric insights from the liver flukes, Opisthorchis and Fasciola

2020 ◽  
Vol 1 ◽  
Author(s):  
Maria João Gouveia ◽  
Maria Y. Pakharukova ◽  
Gabriel Rinaldi ◽  
Viatcheslav A. Mordvinov ◽  
Paul J. Brindley ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Molecular Basis ◽  
Liver Fluke ◽  
Helminth Infection ◽  
Fasciola Hepatica ◽  
Dna Bases ◽  
Liquid Chromatography Mass Spectrometry ◽  
Food Borne ◽  
Liver Flukes ◽  
Compare And Contrast

AbstractEarlier reports revealed oxysterol metabolites of Opisthorchis spp. liver fluke origin conjugated with DNA bases, suggesting that the generation of these DNA-adducts may underlie the mutagenicity and carcinogenicity of the infection with these food-borne pathogens. Here, we employed liquid chromatography-mass spectrometry to investigate, compare and contrast spectrograms of soluble extracts from Fasciola hepatica adult worms from bile ducts of cattle with those from O. viverrini and O.felineus from experimentally infected hamsters. F. hepatica and Opisthorchis spp. shared common compounds including oxysterol-like metabolites, bile acids and DNA-adducts, but the spectrometric profiles of F. hepatica included far fewer compounds than Opisthorchis species. These findings support the postulate that parasitic oxysterol-like metabolites could be related to carcinogenesis associated to infection and they point to a molecular basis for the differences among major groups of liver flukes concerning infection-induced malignancy.

scholarly journals Fascioliasis

2019 ◽  
Vol 2 (1) ◽  
pp. 1-5
Author(s):  
Kittiyod Poovorawan
Keyword(s):  
Aquatic Plants ◽  
Fasciola Hepatica ◽  
Clinical Symptoms ◽  
Intestinal Wall ◽  
Fasciola Gigantica ◽  
Mild Disease ◽  
Drug Of Choice ◽  
Liver Flukes ◽  
Fasciola Spp ◽  
The Right

Fascioliasis is infection by liver flukes, Fasciola spp. The two species that infect humans are Fasciola hepatica and Fasciola gigantica. Other herbivorous animals such as cattle, goats, water buffalo, horses, camels, hogs, rabbits, and deer can be infected with Fasciola spp. Humans acquire Fasciola after ingestion of food and water that are contaminated with Fasciola metacercaria, especially aquatic plants. Fascioliasis is usually mild disease in human. Clinical manifestrations are related with the the phases of infection. In migratory phase, symptoms may occur when metacercariae excyst in the duodenum and young liver flukes migrate through the intestinal wall to the liver capsule and hepatic parenchyma before reaching the bile ducts. Clinical symptoms include abdominal pain in the right hypochondrium, dyspepsia and anorexia, nausea, vomiting, and hepatomegaly. Systemic manifestration such as urticaria and eosinophilia are also common presentation. After Fasciola reside in the biliary tract, most of cases are asymptomatic. Biliary track obstruction can accidentally occur and might lead to cholangitis. Triclabendazole 10 mg/kg as a single dose is the current drug of choice for fascioliasis treatment and triclabendazole two doses of 10 mg/kg for 1 day is effective in severe or persistent infections, with cure rates of 79% and 92%, respectively. Currently, there is no vaccine for prevent Fasciola infection. Proper cooking or cleaning food especially aquatic plants before consumption will prevent accidental Fasciola infection. Adequate mass treatment among infected animals in endemic areas and proper sanitary in the livestock farm are important for disease control.   Figure 1  ภาพถ่ายตัวเต็มวัยพยาธิ Fasciola hepatica (Linnaeus, 1758) (A) และ Fasciola gigantica (Cobbold, 1855) (B)(ได้รับการอนุเคราะห์ภาพจาก รศ.ดร.อุรุษา แทนขำ ภาควิชาปรสิตหนอนพยาธิ คณะเวชศาสตร์เขตร้อน มหาวิทยาลัยมหิดล)                 

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