Anatomical and Morphometric Study of Small and Large Intestine of Bali Cattle

Information on the anatomical and morphometric structure of the digestive system of Bali cattle will provide a clear and precise picture if abnormalities occur in the organs in the digestive system. This study aims to determine the anatomical and morphometric structures of the small intestine and large intestine of Bali cattle. Samples were taken from 14 Bali cattle and based on gross pathology examination declared healthy. The results showed that the average length of the small intestine including the duodenum, jejunum and ileum are 473.07 cm, 529.64 cm, and 363.64 cm; and successive widths are 5.50 cm, 6.00 cm, and 6.11 cm. While the average length of the large intestine which includes the cecum, colon and rectum are 36.78 cm, 371.21, 50.00 cm; while the width are 9.65 cm, 11.47 cm, and 8.85 cm. Jejunum has the longest size in the small intestine, while in the large intestine the longest size is the colon. The right information and data about the digestive system are very important and useful to support further clinical and preclinical research, and the results of this study can be used as a reference in determining the characterization of Bali cattle.

Pesticide Contamination in Feed and Histopathological Properties of Bali Cattle Liver Slaughtered in Pesanggaran Slaughterhouse Denpasar

The number of crop pests that attack agricultural crops caused farmers to take precautions and treatment of crop cultivation by spraying using pesticides. Spraying action by using this pesticide also affects the grasses that grow around it which is a source of food for cattle. This study aims to evaluate basic data from the image of the liver of a Bali cattle that eats grass contaminated with pesticides and is slaughtered in the Pesanggaran slaughterhouse, Denpasar. Histopathological image of the liver of Bali cattle may vary, depending on the level of contamination of various pesticide ingredients that are eaten by the cattle. To see the histopathological changes in the liver of Bali cattle carried out by staining Hematoxylin Eosin (HE) and then it will be examined under a microscope. Observed changes that occur in the form of fatty degeneration, hemorrhage and necrosis. The study concludes is that cattle slaughtered in the Pesanggaran slaughterhouse, Denpasar come from various districts in Bali and the liver of Bali cattle slaughtered in the Pesanggaran Slaughterhouse, Denpasar are mostly exposed to pesticides characterized by histopathological features in the form of fatty degeneration, hemorrhagic and necrosis.

Age-Sex Related in Hematological Values of Sandalwood Pony Horses (Equus caballus) in East Sumba, NTT

Normal hematological values need to be defined for each category of horse, like age and sex to obtain precise diagnostic results. No published data on hematology exist for Sandalwood horses that are extensively reared in East Sumba, NTT. The main objective of this research was to compare the hematological characteristic of horse group based on age and sex. Blood samples from sixteen horses were collected based on sex into male (n=6), female (n=10) and age groups of 2-3 years (n=4) and 6-10 years old (n=12). About 3 ml of blood sample was collected aseptically from jugular vein of each of these animals in sterile vacutainer tube. The following variables were measured: erythrocyte, leucocyte, platelet, hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and mean platelet volume (MPV). These hematological parameters were calculated with the automatic hematology analyzer at Health Laboratory in Kupang, NTT. This result was analyzed by using student T-test and analysis of variance. These results would contribute to better understanding of the hematological indicators for estimating the physiological status of Sandalwood horse in East Sumba as it can be used as valuable information of its health physiological status.

Prevalence and Identification of Tick Infestation in Bali’s Cattle in Badung Regency

Tick is an ectoparasite in cattle that economically very important because it can reduce livestock production and productivity. This study aims to determine the prevalence and to identify types of tick in Bali’s cattle in Badung Regency. The prevalence of tick in relation to gender and age was studied. A total of 285 cattle was examined, of which, 65 (22.8%) cattle were infected by tick. The female cattle showed higher (25%) tick infestation than male cattle (19.05 %) without significant differences (p>0.05). Prevalence of tick was significantly higher (p<0.05) in old cattle, age above 5 years (33.7%), followed by adult cattle, age of 2 -5 years (20.6%), and the least prevalence in young cattle, age under 2 year (13.3%). Two genera of tick were identified from the study. Among the tick, Boophilus sp. 15.17% was the most prevalent tick genus identified, while the rest was Rhipicephalus sp. 7.01 %. Favorable predilection sites for tick were ears, mammary gland, back leg, and neck of the cattle. This study could help in a better understanding of the prevalence of tick patterns and risk factors in cattle populations for the implementation of effective control plans.

Changes in blood calcium concentrations in Bali cattle during the periparturient period

Calcium is a macromineral that plays an important role in the health, production, and reproduction of animals. Calcium requirements in cattle depend on breed, age and parity, path physiological status, body condition score, gestation status, and lactation. During the periparturient period, the cows struggle to maintain calcium homeostasis, and if it is failed, the animal will experience hypocalcemia. As is known hypocalcemia as a predisposing factor for a number of reproductive disorders and infectious diseases. This study aims to investigate changes in blood calcium levels in Bali cattle during the periparturient period. The object of research is nine months pregnant Bali cattle and in good health, kept in cage in a horticultural farming environment. Three ml blood samples were taken through the jugular vein three times, namely in the last three weeks of gestation, on day parturition, and three weeks after calving. Blood samples were processed using the wet digestion method, and calcium concentrations were measured using the flame atomic absorption spectrometric method. The results showed an average blood calcium concentrations of 9.57±1.26 mg/dl, 4.11±0.63 mg/dl, and 8.32±1.23 mg/dl, respectively. Statistical analysis showed blood calcium levels during delivery experienced a very significant decrease. We find new evidence regarding the physiological data of Bali cattle, that at the time of parturition they experienced subclinical hypocalcemia. We concluded that there was a fluctuating change in blood calcium concentrations, where the normal value in the last three weeks of gestation experienced a significant decrease at the parturition and increased after three weeks of calving but has not yet reached the initial condition.

Application of Indonesian's National Standard for Grouping of Bali Cattle with Cluster and Biplot Analysis

Research on the application of the Indonesian National Standard (SNI) of Bali cattle by cluster analysis and biplot aims to provide a visual picture in the form of tables and graphs, so that it is easier and faster and more communicative in making decisions, whether the cows studied are included in class I, class II, or class III based on SNI Bali cattle. This study was conducted on 70-year-old adult female cows of 70 animals raised at the Integrated Farming System (Simantri) in Badung regency. The data obtained were analyzed by cluster analysis and biplot, as variables were shoulder height, body length and chest circumference, while as objects were 70 adult cows and 3 classes of Balinese cattle based on SNI of Bali cattle as object identifiers. The results obtained that the application of SNI for Bali cattle can be done by cluster analysis and biplot and both analyzes give the same results to the grouping of Bali cattle objects based on SNI for Bali cattle. Grouping by cluster analysis is easier to see based on the cluster membership obtained, whereas with biplot analysis provides additional information about correlations and diversity between variables.

Antibody Survey of Bovine Viral Diarrhea in Bali Cattle

Bovine Viral Diarrhea (BVD) or malignant diarrhea in cattle is one of the animal diseases that causes economic losses in the cattle industry worldwide. The biggest economic loss due to infection by Bovine Viral Diarrhea is related to reproductive and calf disorders that continue to transmit the virus to other cattle. This study aims to determine the presence of Bovine Viral Diarrhea antibodies in Bali cattle. The sample uses Bali cattle’s blood from 30 cows that are accommodated in 2 tubes that contain anti-coagulant and which do not contain anti-coagulant. After being processed to get serum, plasma and buffy coat cells, then the samples were examined using the ELISA method and presented descriptively. The results showed that there were positive suspects in Sobangan village, Badung with 8 out of 15 samples (53%) and positive suspects in the village of Payangan, Gianyar with 3 out of 15 samples (20%). Positive results are influenced by biosecurity of each type of sample farm, in Sobangan village is a large farm so biosecurity is difficult to implement and the spread of disease is faster than in Payangan village that have small farm type.

The Number and Types of Bacteria in the Bali Cattle’s Vagina during Estrus and Pregnant

Bali cattle have very good potential for meat and calf supply, because they have good adaptability with good quality reproductive properties. However, good reproductive nature is threatened by diseases caused by bacteria. This study aims to determine the number and types of bacteria in the vagina of Bali cattle that are estrus and pregnant. Sample using vaginal swabs from 30 cows. The sample consisted of 10 swabs from non-estrus and not pregnant (control / normal) cattle, 10 swabs from estrus cows, and 10 swabs from pregnant cows. After the swab is carried out using a cotton bud, the results of the swab are stored in Stuart transport media. Samples planted in blood media to be identified and the number of colonies counted, then followed by Gram staining and biochemical tests then the results were presented descriptively. The result is the highest number of estrous cow colonies (1034 colonies), compared to non-estrous and non-pregnant cows (407 colonies) and pregnant (376 colonies). This type of bacteria is dominated by Klebsiella sp. (42.85%), Eschericia coli (28.7%), Streptococcus sp. (14.28%) and Bacillus sp. (14.28%).

Lactic Acid Profile of Bali cattle during Rest Period Before Slaughtered

The animal products quality is influenced the physiological compounds in the blood, for example lactic acid. If blood lactic acid levels increase before slaughtering will be accelerate the rigor mortis of meat, thus faster handling is needed. Blood lactic acid also affects the appearance of flesh color of meat. The condition of animals before slaughter (rest period in quarantine) is very influential on rigor mortis. It is important to research blood lactic acid levels before animals are slaughtered. This research was conducted to determine the blood lactic acid levels of bali cattle during the rest period before the animal was slaughtered (ante mortem), so it can be predicted the quality of meat products. Purposive was chosen as a sampling method using elisa test as a determination of lactic acid levels. Fourtysamples of blood taken from cows to be slaughtered at Sanggaran Animal Abattoir, Denpasar, Bali. The results showed that the blood levels of lactic acid in cattle would be cut normally. This shows the quality of resting cattle before slaughter is good.

The Change in External Genital and Estrogen Level of Bali Cattle During Estrus

The successful application of reproductive technology requires knowledge of estrus cycle and the ability to detect estrus of Bali cattle. Estrus in cattle lasts for 8-30 hours showed by the acceptance of males for copulation. In this phase, estrogen levels reach maximum levels. High levels of estrogen cause signs of estrus, and changes in the reproductive organs. This study aims to determine estrogen levels during estrus and external genital changes during estrus. This research was an observational analytic study with cross-sectional study design. The samples used in this study were Bali cattle during estrus. The samples had healthy status without any signs of disease. Blood samples were taken through the jugular vein were then centrifuged to obtain serum. The obtained serum was examined for estrogen hormone levels. Mechanical measurements of hormone levels checked by using the Direct Elisa, Double Antibody Sandwich. Blood samples were collected from several SIMANTRI in Mengwi District, Badung Regency. The results showed in Bali cattle external genitals change when estrus, namely redness of the vaginal mucosa, discharge from the vagina and swelling of the vulva. While estrogen levels when estrus is 69.80 pg/ ml. Further research needs to be continued to measure the quality of ovulation after estrus.