The In-vitro Effect of Indomethacin on Uterine Muscle Contraction Stimulated by Acetone Leaf Extract of Anogeissus Leiocarpus

There have been a number of reports concerning the role of dietary fiber in hampering the diffusion of glucose and lowering the postprandial serum glucose. The present study investigates the effect of viscous aqueous leaf extract of Abroma augusta L (Family: Sterculiaceae, Bengali name: Ulatkambal, English name: Devil's cotton, DC) on the diffusion of glucose in vitro. Different mixtures were prepared using varying concentrations of sodium carboxymethylcellulose (Na-CMC) and aqueous extract of A. augusta with a fixed concentration of glucose. The diffusion of glucose from these systems into the outer medium through the ultra-fine membrane was measured. The results showed that both Na-CMC and aqueous extract of ulatkambal significantly (p<0.05) reduced the diffusion of glucose compared to control in a concentration-dependent manner. The result of this study suggested that dietary fiber present in the aqueous leaf extract of A. augusta may be potentially effective in the management of type 2 diabetes mellitus by reducing post-prandial glucose absorption from the gastrointestinal tract. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14486 Bangladesh Pharmaceutical Journal 16(1): 21-26, 2013

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Extracellular Calcium and Induction of Uterine Muscle Contraction by Aqueous Ethanolic Leaf Extract of Mucuna pruriens

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v24i2.6 ◽

2020 ◽

Vol 24 (2) ◽

pp. 231-235

Author(s):

B. Francis ◽

C.N. Uchendu

Keyword(s):

Muscle Contraction ◽

Leaf Extract ◽

Smooth Muscle Contraction ◽

Mucuna Pruriens ◽

Physiological Salt Solution ◽

Cellular Processes ◽

Uterine Muscle ◽

Wide Range ◽

Intracellular Storage ◽

Ethanolic Leaf Extract

Calcium (Ca2+) serves as an essential signaling molecule in biological systems, regulating a wide range of cellular processes of which uterine smooth muscle contraction is among. The present study was designed to evaluate the involvement of Ca2+ on isolated uterine muscle contraction induced by aqueous ethanolic leaf extract of Mucuna pruriens (M. pruriens). Uterine muscle contraction induced by the extract was concentration-dependent and was completely abolished (100%; P<0.05) in nominally Ca2+ -free physiological salt solution and in solutions containing (EGTA 1.5 mmol), lanthanium chloride (1.5 and 3 mmol), caffeine (3and 4.4 mmol) and verapamil (0.007-0.14 μmol). It is concluded that the inability of the extract to produce contractions in Ca2+ -free media, indicates that it lacks the ability to mobilize calcium from intracellular storage sites. Hence, its uterine stimulatory property is therefore solely dependent on extracellular Ca2+. Keywords: Calcium, Mucuna pruriens, Uterus, Contraction.

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