scholarly journals Extracellular Calcium and Induction of Uterine Muscle Contraction by Aqueous Ethanolic Leaf Extract of Mucuna pruriens

2020 ◽  
Vol 24 (2) ◽  
pp. 231-235
Author(s):  
B. Francis ◽  
C.N. Uchendu
Keyword(s):  
Muscle Contraction ◽  
Leaf Extract ◽  
Smooth Muscle Contraction ◽  
Mucuna Pruriens ◽  
Physiological Salt Solution ◽  
Cellular Processes ◽  
Uterine Muscle ◽  
Wide Range ◽  
Intracellular Storage ◽  
Ethanolic Leaf Extract

Calcium (Ca2+) serves as an essential signaling molecule in biological systems, regulating a wide range of cellular processes of which uterine smooth muscle contraction is among. The present study was designed to evaluate the involvement of Ca2+ on isolated uterine muscle contraction induced by aqueous ethanolic leaf extract of Mucuna pruriens (M. pruriens). Uterine muscle contraction induced by the extract was concentration-dependent and was completely abolished (100%; P<0.05) in nominally Ca2+ -free physiological salt solution and in solutions containing (EGTA 1.5 mmol), lanthanium chloride (1.5 and 3 mmol), caffeine (3and 4.4 mmol) and verapamil (0.007-0.14 μmol). It is concluded that the inability of the extract to produce contractions in Ca2+ -free media, indicates that it lacks the ability to mobilize calcium from intracellular storage sites. Hence, its uterine stimulatory property is therefore solely dependent on extracellular Ca2+. Keywords: Calcium, Mucuna pruriens, Uterus, Contraction.

scholarly journals Mechanism of Spasmogenic Activity Stimulated by Aqueous Ethanolic Leaf Extract of Mucuna pruriens on Isolated Uterine Muscle of Albino Rats

2019 ◽  
pp. 1-10
Author(s):  
B. Francis ◽  
C. N. Uchendu ◽  
R. I. Obidike
Keyword(s):  
Smooth Muscle ◽  
Leaf Extract ◽  
Mucuna Pruriens ◽  
Albino Rats ◽  
Organ Bath ◽  
Atropine Sulphate ◽  
Uterine Smooth Muscle ◽  
Uterine Muscle ◽  
Dose Dependent ◽  
Ethanolic Leaf Extract

Aims: To investigate the effect of aqueous ethanolic leaf extract of this medicinal plant on isolated uterine smooth muscle strips of the rat and to determine its mechanism of action. Study Design:  Laboratory-experimental design was used in this study. Place and Duration of Study: The study was carried out in the Department of Veterinary Physiology and Biochemistry of Michael Okpara University of Agriculture, Umudike, Nigeria, Department of Pharmacology and Toxicology of the Faculty of Pharmacy, University of Nigeria, Nsukka, and the Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka, Nigeria between June and October 2014. Methodology: Fresh leaves of Mucuna pruriens were identified and collected by a taxonomist from Nsukka, Nigeria. The leaves were then air dried and pulverized into powder. This was then subjected to cold extraction using petroleum ether (70-90) and 70% aqueous ethanol, after which the extract was left to dry at room temperature. Estrogenised uterine strips (12mm) were harvested from non-pregnant, sexually matured albino rats (180 g -250 g) and suspended in a 35ml organ bath containing Krebs’ physiological salt solution. The organ bath was connected to an isometric electronic force displacement transducer and a physiograph. Drugs such as Salbutamol, Isoprenaline, Adrenaline, Propranolol, Atipamezole and Prazosin were used as either agonists or antagonists to determine the mechanism of action of the extract. Atropine sulphate and Cyproheptadine were also used as test drugs. Concentrations of these drugs presented in the body of this work represent the final nutrient bath concentrations. Results: M. pruriens caused a dose -dependent increase in uterine muscle contraction with an EC50 of 0.88 mg/ml, n=4. The contraction was unaffected by atropine sulphate (0.042 µmol), but abolished by salbutamol (0.012-0.4 µmol), isoprenaline (0.06-0.23 µmol), and adrenaline (16 nmol). The uterine muscle contractions were enhanced by propranolol (1 µmol) in a dose- dependent manner. Prazosin (0.069-0.14 µmol) and atipamezole (3.3-13.7 nmol) were unable to abolish contractions stimulated by the extract. However, 0.2 µmol of cyproheptadine caused 80% suppression of the extract –induced uterine contraction Conclusion: It is concluded that aqueous ethanolic leaf extract of M. pruriens, has ability to cause uterine smooth muscle contraction hence, justifies its reported use traditionally as a uterine stimulant. This contraction is most likely exerted via the 5-HT receptor activation (activated by low concentrations of serotonin).

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

Antihypertensive and Cardio-Protective Potentials of Ethanolic Leaf Extract of Mucuna Pruriens in Male Albino Rats

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Joseph Minari ◽  
G.E Nwosu ◽  
E.E Agho ◽  
B.O Sholaja
Keyword(s):  
Leaf Extract ◽  
Treatment Options ◽  
Mucuna Pruriens ◽  
White Blood Count ◽  
Control Group ◽  
Albino Rats ◽  
Protective Effects ◽  
Standard Drug ◽  
Hematological Indices ◽  
Ethanolic Leaf Extract

Hypertension is a risk factor for a variety of morbidities, especially stroke, myocardial infarction, and the development of congestive heart failure, as well as death. Several treatment options are used for treatment of hypertension; however, a lot of short comings have been associated with them. In order to proffer a possibly better and cheaper means of preventing hypertension, this study aims at investigating the antihypertensive and cardio-protective effects of the ethanolic leaf extract of Mucuna pruriens. Thirty-six male albino rats were used for the study. Oral administration of 8% salt diet was used to induce hypertension which significantly increased blood pressure and oxidative stress in the hypertensive animals compared to the control group. The phytochemical screening of the extract was carried out, the weight of the experimental animals was monitored, hematological indices was assessed, and systolic and diastolic rates were evaluated at different concentrations of the extract. The screening indicated the presence of tannin, saponins, flavonoids, terpenoids, and phenols while reducing sugars, cardiac glycosides, glycoside, alkaloids and steroids were absent. The weight of the liver of rats given standard drug, 150 mg/ml of the extract, 250 mg/ml of the extract and untreated groups were significant lower (p<0.05) when compared with the control group while the group administered with 100 mg/ml was higher. The white blood count

Regulation of Smooth Muscle Contraction by the Epithelium: Role of Prostaglandins

Physiology ◽  
2011 ◽  
Vol 26 (3) ◽  
pp. 156-170 ◽  
Author(s):  
Ye Chun Ruan ◽  
Wenliang Zhou ◽  
Hsiao Chang Chan
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Muscle Contraction ◽  
Cell Types ◽  
Smooth Muscle Contraction ◽  
Organ Systems ◽  
Wide Range ◽  
Important Regulator

As an analog to the endothelium situated next to the vascular smooth muscle, the epithelium is emerging as an important regulator of smooth muscle contraction in many vital organs/tissues by interacting with other cell types and releasing epithelium-derived factors, among which prostaglandins have been demonstrated to play a versatile role in governing smooth muscle contraction essential to the physiological and pathophysiological processes in a wide range of organ systems.

Contribution of intracellular calcium to gallbladder smooth muscle contraction

1990 ◽  
Vol 259 (1) ◽  
pp. G1-G5 ◽  
Author(s):  
L. M. Renzetti ◽  
M. B. Wang ◽  
J. P. Ryan
Keyword(s):  
Smooth Muscle ◽  
Sarcoplasmic Reticulum ◽  
Ethylene Glycol ◽  
Muscle Contraction ◽  
Contractile Response ◽  
Smooth Muscle Contraction ◽  
Physiological Salt Solution ◽  
Tetraacetic Acid ◽  
Optimal Length ◽  
Ethanesulfonic Acid

Studies were performed to evaluate the contribution of intracellular Ca2+ to gallbladder smooth muscle contraction under acetylcholine (ACh) or potassium stimulation. Gallbladder smooth muscle strips from adult guinea pigs were placed in tissue baths containing N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES)-buffered physiological salt solution (PSS) and set to optimal length for contraction (Lo). The results were as follows, 1) A 20-min equilibration in zero Ca2(+)-0.1 mM ethylene glycol-bis( beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) PSS virtually abolished the response to potassium but not to ACh. 2) Substitution of strontium, an inhibitor of intracellular Ca2+ release, for Ca2+ significantly decreased the contractile response to ACh (3 X 10(-5), 10(-4), and 3 X 10(-4) M). Strontium had no effect on the response to 40 and 80 mM potassium. 3) Intracellular Ca2+ depletion significantly decreased gallbladder smooth muscle contraction to ACh (10(-4) M) but had no effect on the response to potassium (80 mM). 4) Ryanodine, a compound that inhibits Ca2+ storage by the sarcoplasmic reticulum, significantly decreased the contractile response to ACh (10(-4) M) but not to potassium (80 mM). These data support the observation that the use of intracellular Ca2+ by gallbladder smooth muscle for contraction is agonist dependent.

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY AND ESTIMATION OF TOTAL PHENOL AND FLAVONOID CONTENT OF ETHANOLIC LEAF EXTRACT OF IRIS KASHMIRIANA

2017 ◽  
Vol 10 (8) ◽  
pp. 309
Author(s):  
Shah Khalid ◽  
Aparna Alia ◽  
Shrivastava Pn ◽  
Muzafar Akbar Rather ◽  
Muzafar Ah Sheikh
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Reducing Power ◽  
Total Phenol ◽  
Flavonoid Content ◽  
Dpph Assay ◽  
Wide Range ◽  
Ethanolic Leaf Extract ◽  
In Vitro Antioxidant Activity

Objective: The main aim of this study was to determine the in vitro antioxidant activity of Iris kashmiriana ethanolic leaf extract and also total phenol and flavonoid content was evaluated.Methods: Total phenol content (TPC) was determined by Folin–Ciocalteu method, total flavonoid content (TFC) was estimated by aluminum trichloride spectrophotometer method. Furthermore, antioxidant activity was revealed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydrogen peroxide (H2O2) scavenging activity, and reducing power assay.Results: The ethanolic leaf extract of I. kashmiriana showed TPC of 13.25±0.57 μg/100 μg gallic acid equivalents and TFC of 33.61±3.37 μg/100 μg rutin equivalents. The DPPH assay revealed IC50 of 0.418 mg/ml and for H2O2 radical scavenging IC50 was 0.476 mg/ml for the plant extract while as reducing power assay revealed concentration-dependent absorption values which clearly determine the antioxidant property of plant.Conclusion: From the results, it is apparent that I. kashmiriana ethanolic leaf extract possessed potential antioxidant activity which can be used to cure wide range of diseases.

Sign in / Sign up

Export Citation Format

Share Document