In Vitro Anthelmintic Effect of some Medicinal Plants against Haemonchus Contortus

2015 ◽  
Vol 5 (1) ◽  
pp. 1162 ◽  
Author(s):  
Sonia Akther ◽  
Anita Dey ◽  
Shahadat Hossain ◽  
Taposhi Dey ◽  
Nurjahan Begum
Keyword(s):  
Medicinal Plants ◽  
Haemonchus Contortus ◽  
Anthelmintic Effect

Anthelmintic effect of four extracts obtained from Caesalpinia coriaria foliage against the eggs and larvae of Haemonchus contortus

2021 ◽  
Vol 30 (3) ◽  
Author(s):  
Diana Rojas-Morales ◽  
Jaime Cubides-Cárdenas ◽  
Andrea Constanza Montenegro ◽  
Carlos Alberto Martínez ◽  
Ronnal Ortíz-Cuadros ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Haemonchus Contortus ◽  
Probit Analysis ◽  
South American ◽  
Egg Hatching ◽  
Acetone Water ◽  
Acetone Extracts ◽  
Eggs And Larvae ◽  
Anthelmintic Effect

Abstract To investigate the in vitro anthelmintic efficacy of dividivi (Caesalpinia coriaria), a traditional medicinal plant used in Central America and the northern part of South America, extracts from the foliage of this plant were subjected to the egg hatching test (EHT) and larval exsheathment inhibition test (LEIT), against Haemonchus contortus. Four different extracts were evaluated: acetone-water (AW), methanol-water (MW), acetone-water-dichloromethane (AWD) and methanol-water-dichloromethane (MWD). The concentrations used for the EHT and LEIT tests ranged from 500 to 4000 µg mL-1 and six repetitions per concentration. The effective concentrations (EC50) were calculated using Probit analysis. The EC50 for EHT were 2947.0, 3347.0, 3959.6 and 4538.7 µg mL-1 for MWD, MW, AW and AWD, respectively. The EC50 for LEIT were 2883.4, 5927.4, 9876.3 and 9955.4 µg mL-1 for AWD, AW, MWD and MW, respectively. The methanol extracts were the most effective in inhibiting the hatching of eggs, while the acetone extracts showed efficacy in inhibiting larval exsheathment. This study explains the importance that C. coriaria has as a medicinal plant in Central and South American countries.

scholarly journals In vitro nematicidal effect of Chenopodium ambrosioides and Castela tortuosa n-hexane extracts against Haemonchus contortus (Nematoda) and their anthelmintic effect in gerbils

2018 ◽  
Vol 93 (04) ◽  
pp. 434-439 ◽  
Author(s):  
A. Zamilpa ◽  
C. García-Alanís ◽  
M.E. López-Arellano ◽  
V.M. Hernández-Velázquez ◽  
M.G. Valladares-Cisneros ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Adult Stage ◽  
Chenopodium Ambrosioides ◽  
Anthelmintic Efficacy ◽  
Hexane Extracts ◽  
Parasitic Burden ◽  
The Individual ◽  
Anthelmintic Effect

AbstractThe in vitro nematicidal effect of Chenopodium ambrosioides and Castela tortuosa n-hexane extracts (E-Cham and E-Cato, respectively) on Haemonchus contortus infective larvae (L3) and the anthelmintic effect of these extracts against the pre-adult stage of the parasite in gerbils were evaluated using both individual and combined extracts. The in vitro confrontation between larvae and extracts was performed in 24-well micro-titration plates. The results were considered 24 and 72 h post confrontation. The in vivo nematicidal effect was examined using gerbils as a study model. The extracts from the two assessed plants were obtained through maceration using n-hexane as an organic agent. Gerbils artificially infected with H. contortus L3 were treated intraperitoneally with the corresponding extract either individually or in combination. The results showed that the highest individual lethal in vitro effect (96.3%) was obtained with the E-Cham extract at 72 h post confrontation at 40 mg/ml, followed by E-Cato (78.9%) at 20 mg/ml after 72 h. The highest combined effect (98.7%) was obtained after 72 h at 40 mg/ml. The in vivo assay showed that the individual administration of the E-Cato and E-Cham extracts reduced the parasitic burden in gerbils by 27.1% and 45.8%, respectively. Furthermore, the anthelmintic efficacy increased to 57.3% when both extracts were administered in combination. The results of the present study show an important combined nematicidal effect of the two plant extracts assessed against L3 in gerbils.

In vitro anthelmintic activity of crude extracts of selected medicinal plants against Haemonchus contortus from sheep

2012 ◽  
Vol 87 (2) ◽  
pp. 174-179 ◽  
Author(s):  
M. Ahmed ◽  
M.D. Laing ◽  
I.V. Nsahlai
Keyword(s):  
Medicinal Plants ◽  
Haemonchus Contortus ◽  
Allium Sativum ◽  
Anthelmintic Activity ◽  
Ananas Comosus ◽  
Lespedeza Cuneata ◽  
Aloe Ferox ◽  
Ethanol Extracts

AbstractEthanol extracts of 25 plant species were screened for anthelmintic effects against Haemonchus contortus. Ethanol extracts of each plant were used at various concentrations (10, 20 and 30%) to treat 10-day faecal cultures, incubated at 27°C with control cultures which were treated with ethanol for 48 h. Five plants with high efficacies (Ananas comosus, Aloe ferox, Allium sativum, Lespedeza cuneata and Warburgia salutaris) were selected from the first screening for further investigation using ethanol, dichloromethane and water extracts at four concentrations (2.5, 5, 10 and 20%). Ethanol was the most effective solvent. Larval counts decreased with increasing extract concentrations, of which 10 and 20% had similar effects. Lespedeza cuneata caused more than 70% mortality at all concentrations. However, there remains a need to assess in vivo efficacy of these plants.

In-vitro anthelmintic activity of three Bangladeshi plants against Paramphistomum cervi and Haemonchus contortus

2015 ◽  
Vol 12 (2) ◽  
Author(s):  
Md. Khirul Islam ◽  
Md. Afjalus Siraj ◽  
Asit Baron Sarker ◽  
Sanjib Saha ◽  
Imran Mahmud ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Haemonchus Contortus ◽  
Leaf Extract ◽  
Anthelmintic Activity ◽  
Therapeutic Tool ◽  
Lead Compounds ◽  
Continuous Evaluation ◽  
The World ◽  
Paramphistomum Cervi

Abstract: Conventional plant-based therapies act as an important therapeutic tool for the treatment of worm infections all over the world and continuous evaluation of medicinal plants to find new potential lead compounds should be carried out.:: Among all three concentrations (25, 50, and 100 mg/mL), the hydroalcoholic leaf extract of:

In-vitro Anthelmintic Efficacy of Some Kashmiri Medicinal Plants against Haemonchus contortus

2015 ◽  
Vol 5 (5) ◽  
pp. 73 ◽  
Author(s):  
Shahjahan Alam ◽  
Tarun Sarkar ◽  
Irfan Shah ◽  
Imtiyaz Reshi ◽  
Mazar Shah
Keyword(s):  
Medicinal Plants ◽  
Haemonchus Contortus ◽  
Anthelmintic Efficacy

Anthelmintic effect and tissue alterations induced in vitro by hydrolysable tannins on the adult stage of the gastrointestinal nematode Haemonchus contortus

2019 ◽  
Vol 266 ◽  
pp. 1-6 ◽  
Author(s):  
Perla María del Carmen Acevedo-Ramírez ◽  
Claudia Hallal-Calleros ◽  
Iván Flores-Pérez ◽  
Fernando Alba-Hurtado ◽  
María Berenit Mendoza-Garfías ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Adult Stage ◽  
Gastrointestinal Nematode ◽  
Hydrolysable Tannins ◽  
Tissue Alterations ◽  
Anthelmintic Effect
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