Relationship between Bone Expansion in Odontogenic Keratocyst and Mandibular Cortical Bone

AbstractThe study of galectin-3 is complicated by its ability to function both intracellularly and extracellularly. While the mechanism of galectin-3 secretion is unclear, studies have shown that the mutation of a highly conserved arginine to a serine in human galectin-3 (LGALS3-R186S) blocks glycan binding and secretion. To gain insight into the roles of extracellular and intracellular functions of galectin-3, we generated mice with the equivalent mutation (Lgals3-R200S) using CRISPR/Cas9-directed homologous recombination. Consistent with a reduction in galectin-3 secretion, we observed significantly reduced galectin-3 protein levels in the plasma of heterozygous and homozygous mutant mice. We observed a similar increased bone mass phenotype in Lgals3-R200S mutant mice at 36 weeks as we previously observed in Lgals3-KO mice with slight variation. Like Lgals3-KO mice, Lgals3-R200S females, but not males, had significantly increased trabecular bone mass. However, only male Lgals3-R200S mice showed increased cortical bone expansion, which we had previously observed in both male and female Lgals3-KO mice and only in female mice using a separate Lgals3 null allele (Lgals3). These results suggest that the trabecular bone phenotype of Lgals3-KO mice was driven primarily by loss of extracellular galectin-3. However, the cortical bone phenotype of Lgals3-KO mice may have also been influenced by loss of intracellular galectin-3. Future analyses of these mice will aid in identifying the cellular and molecular mechanisms that contribute to the Lgals3-deficient bone phenotype as well as aid in distinguishing the extracellular vs. intracellular roles of galectin-3 in various signaling pathways.

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Enhanced cortical bone expansion in Lgals3-deficient mice during aging

Bone Research ◽

10.1038/s41413-017-0003-6 ◽

2018 ◽

Vol 6 (1) ◽

Cited By ~ 2

Author(s):

Kevin A. Maupin ◽

Kevin Weaver ◽

Alexis Bergsma ◽

Cheryl Christie ◽

Zhendong A. Zhong ◽

...

Keyword(s):

Cortical Bone ◽

Bone Expansion ◽

Deficient Mice

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Desmoplastic Ameloblastoma of the Mandible

Journal of Nepal Medical Association ◽

10.31729/jnma.2282 ◽

2013 ◽

Vol 52 (191) ◽

Author(s):

Soniya Adyanthaya ◽

Pushparaja Shetty

Keyword(s):

Cortical Bone ◽

Radiographic Finding ◽

Odontogenic Tumor ◽

Anterior Portion ◽

Radiographic Findings ◽

Anatomical Distribution ◽

Bone Expansion ◽

Radiolucent Lesion

The Desmoplastic Ameloblastoma exhibits important differences in anatomical distribution, histologicappearance and radiographic findings compared with the classic type of ameloblastoma. We describea case of desmoplastic variant of ameloblastoma localized in the mandibular anterior portion of thejaw, extending posteriorly in the left side. The tumor was expansile, slowly growing and painless.The radiographic finding showed diffuse, radiolucent lesion with cortical bone expansion. Surgicalexcision was performed and follow-up examination done after two years disclosed no recurrence.The purpose of this article is to assist in better understanding of this variant of odontogenic tumor._______________________________________________________________________________________Keywords: Ameloblastoma; desmoplasia; mandible; odontogenic tumor.

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Central Odontogenic Myxoma: A Radiographic Analysis

International Journal of Dentistry ◽

10.1155/2021/1093412 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Ahmad Badruddin Ghazali ◽

Raweewan Arayasantiparb ◽

Rachai Juengsomjit ◽

Aroonwan Lam-ubol

Keyword(s):

Statistical Analysis ◽

Cortical Bone ◽

Root Resorption ◽

Treatment Decisions ◽

Periosteal Reaction ◽

Histopathological Diagnosis ◽

Radiographic Analysis ◽

Odontogenic Myxoma ◽

Exact Test ◽

Bone Expansion

Objective. This study aimed to determine the radiographic characteristics of odontogenic myxomas (OMs) and their associations. Materials and Methods. The study enrolled radiographs of patients taken between 2005 and 2019 with a confirmed histopathological diagnosis of central OM. OM radiographic features were evaluated, including location, border, locularity, involved area, the number of included teeth, root resorption, tooth displacement, bone expansion, bone perforation, and periosteal reaction. Fisher’s exact test was used for statistical analysis. Results. Significant associations were found between the OM border and the affected jaw ( p = 0.036 ), locularity ( p = 0.036 ), involved areas ( p = 0.009 ), and bone perforation ( p = 0.036 ). OMs with an ill-defined border were associated with maxillary lesions, multilocularity, dentate areas, and cortical bone perforation. The number of included teeth (2 or fewer or 3 or more) was significantly associated with locularity ( p = 0.010 ), involved area ( p = 0.045 ), and bone expansion ( p = 0.010 ). Larger OMs including 3 or more teeth, were associated with a multilocular appearance, dentate areas, and bone expansion. Conclusion. The border of OM and the number of included teeth are related to other radiographic appearances. Understanding these relationships could help in treatment decisions and help better understand the nature of OM.

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Decreased body weight in young Osterix-Cre transgenic mice results in delayed cortical bone expansion and accrual

Transgenic Research ◽

10.1007/s11248-011-9581-z ◽

2011 ◽

Vol 21 (4) ◽

pp. 885-893 ◽

Cited By ~ 61

Author(s):

Rachel A. Davey ◽

Michele V. Clarke ◽

Stephen Sastra ◽

Jarrod P. Skinner ◽

Cherie Chiang ◽

...

Keyword(s):

Body Weight ◽

Transgenic Mice ◽

Cortical Bone ◽

Bone Expansion

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Higher 24-h urinary free cortisone, but not cortisol is associated with impaired cortical bone status at the proximal radius in healthy children

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-0034-1372057 ◽

2014 ◽

Vol 122 (03) ◽

Author(s):

L Shi ◽

A Sánchez-Guijo ◽

MF Hartmann ◽

E Schönau ◽

SA Wudy ◽

...

Keyword(s):

Cortical Bone ◽

Healthy Children ◽

Bone Status ◽

Proximal Radius

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Pullout Strength of Screws in Foal Third Metacarpal Bone after Overdrilling a 4.5 mm Hole

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.1055/s-0038-1632548 ◽

1998 ◽

Vol 11 (04) ◽

pp. 200-204 ◽

Cited By ~ 1

Author(s):

K. Kelly ◽

G. S. Martin ◽

D. J. Burba ◽

S. A. Sedrish ◽

R. M. Moore

Keyword(s):

Cortical Bone ◽

Metacarpal Bone ◽

Pullout Strength ◽

Cancellous Screw ◽

Bone Screw ◽

Screw Insertion ◽

Holding Power ◽

Cortical Screw ◽

Metaphyseal Bone

SummaryThe purpose of the study was to determine and to compare the in vitro pullout strength of 5.5 mm cortical versus 6.5 mm cancellous bone screws inserted in the diaphysis and metaphysis of foal third metacarpal (MCIII) bones in threaded 4.5 mm cortical bone screw insertion holes that were then overdrilled with a 4.5 mm drill bit. This information is relevant to the selection of a replacement screw if a 4.5 mm cortical screw is stripped during orthopaedic surgery. In vitro pullout tests were performed in two independent cadaver studies, each consisting of 12 foal MCIII bones. Two 4.5 mm cortical screws were placed either in the mid-diaphysis (study 1) or distal metaphysis (study 2) of MCIII bones. The holes were then overdrilled with a 4.5 mm bit and had either a 5.5 mm cortical or a 6.5 mm cancellous screw inserted; screw pullout tests were performed at a rate of 0.04 mm/s until screw or bone failure occurred.The bone failed in all of the tests in the diaphyseal and metaphyseal bone. The holding power for 6.5 mm cancellous screws was significantly (p <0.05) greater than for 5.5 mm cortical screws in both the diaphysis and metaphysis. There was not any difference in the holding power of screws in either the diaphysis or the metaphysis between proximal and distal screw holes.If a 4.5 mm cortical bone screw strips in MCIII diaphyseal or metaphyseal bone of foals, a 6.5 mm cancellous screw would provide greater holding power than a 5.5 mm cortical screw.In order to provide information regarding selection of a replacement screw if a 4.5 mm cortical screw is stripped, the in vitro pullout strength was determined for 5.5 mm cortical and 6.5 mm cancellous screws inserted in third metacarpal diaphyseal and metaphyseal bone of foals in which threaded 4.5 mm cortical bone screw insertion holes had been overdrilled with a 4.5 mm bit. The holding power of the 6.5 mm cancellous screw was significantly greater than the 5.5 mm cortical screw in both the diaphysis and metaphysis of foal third metacarpal bone. Thus, it appears that if a 4.5 mm cortical screw is stripped during orthopaedic surgery in foals, a 6.5 mm cancellous screw would provide superior holding power.

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