scholarly journals Somatic embryogenesis from leaf explants of Tagetes erecta L.

2017 ◽  
Vol 34 (4) ◽  
pp. 187-192 ◽  
Author(s):  
Pablo Emilio Vanegas Espinoza ◽  
Israel Benítez-García ◽  
Annel Lizeth Leyva Peralta ◽  
Octavio Paredes-López ◽  
Alma Angélica Del Villar-Martínez
Keyword(s):  
Somatic Embryogenesis ◽  
Leaf Explants ◽  
Tagetes Erecta

scholarly journals Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 70
Author(s):  
Ana D. Simonović ◽  
Milana M. Trifunović-Momčilov ◽  
Biljana K. Filipović ◽  
Marija P. Marković ◽  
Milica D. Bogdanović ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Developmental Plasticity ◽  
Leaf Explants ◽  
Arabinogalactan Proteins ◽  
Culture Conditions ◽  
Current Status ◽  
Special Focus ◽  
Root Explants ◽  
Centaurium Erythraea

Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum of secondary metabolites with confirmed healing properties. Centaury is an emerging model in plant developmental biology due to its vigorous regenerative potential and great developmental plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic embryos. When SE is initiated from centaury root explants, the process occurs spontaneously (on hormone-free medium), directly (without the callusing phase), and the somatic embryos are of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is indirect (preceded by callusing). Histological observations and culture conditions are compared in these two systems. The changes in antioxidative enzymes were followed during SE from the leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were analyzed using a variety of approaches. The newest and preliminary results, including centaury transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed as perspectives of centaury research.

scholarly journals WUSCHEL Overexpression Promotes Callogenesis and Somatic Embryogenesis in Medicago truncatula Gaertn

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 715
Author(s):  
Aline Kadri ◽  
Ghislaine Grenier De March ◽  
François Guerineau ◽  
Viviane Cosson ◽  
Pascal Ratet
Keyword(s):  
Somatic Embryogenesis ◽  
Medicago Truncatula ◽  
Transformation Efficiency ◽  
Genetic Manipulation ◽  
Leaf Explants ◽  
Promising Tool ◽  
Limiting Step ◽  
Wus Gene ◽  
Multiple Signaling ◽  
Regeneration Systems

The induction of plant somatic embryogenesis is often a limiting step for plant multiplication and genetic manipulation in numerous crops. It depends on multiple signaling developmental processes involving phytohormones and the induction of specific genes. The WUSCHEL gene (WUS) is required for the production of plant embryogenic stem cells. To explore a different approach to induce somatic embryogenesis, we have investigated the effect of the heterologous ArabidopsisWUS gene overexpression under the control of the jasmonate responsive vsp1 promoter on the morphogenic responses of Medicago truncatula explants. WUS expression in leaf explants increased callogenesis and embryogenesis in the absence of growth regulators. Similarly, WUS expression enhanced the embryogenic potential of hairy root fragments. The WUS gene represents thus a promising tool to develop plant growth regulator-free regeneration systems or to improve regeneration and transformation efficiency in recalcitrant crops.

scholarly journals In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
Dwi Hapsoro ◽  
Rahmadyah Hamiranti ◽  
Yusnita Yusnita
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Leaf Explants ◽  
Regeneration Medium ◽  
Embryogenic Calli ◽  
Robusta Coffee ◽  
Primary Callus ◽  
Ascorbic Acids ◽  
Superior Clones

Abstract. Hapsoro D, Hamiranti R, Yusnita Y. 2020. In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media. Biodiversitas 21: 3811-3817. This study aimed to investigate the effects of genotypes and primary callus induction media on somatic embryogenesis of superior robusta coffee clones of Lampung. Leaf explants of clones Tugusari, Komari, Tugino, and Wanto were cultured on two types of primary callus induction media (PCIM). PCIM1 consisted of half-strength MS salts, 30 gL-1 sucrose, added with (mgL-1) 0.1 thiamine-HCl, 0.5 nicotinic acids, 0.5 pyridoxine-HCl, 100 Myo-inositol, 200 ascorbic acids, 150 citric acids, and 1 benzyl adenine. PCIM2 consisted of NPCM salts, 30 gL-1 sucrose, added with (mgL-1) 15 thiamine-HCl, 1 nicotinic acid, 1 pyridoxine-HCl, 2 glycines, 130 Myo-inositol, 200 ascorbic acids, 150 citric acids, 1 2,4-dichlorophenoxyacetic acid, and 2 thidiazuron. The highest percentage (100%) of primary callus formation was found in Komari and Wanto clones. PCIM2 resulted in more primary calli than PCIM1. When subcultured to embryogenic callus induction medium, primary calli of clone Komari and Wanto developed into a high percentage of embryogenic calli, while those of the other two turned brown and died. PCIM2-derived primary calli developed into more embryogenic calli. When subcultured on somatic embryo (SE) regeneration medium, these calli underwent the formation of SE of various stages. When subcultured to plant regeneration medium, these SEs developed into plantlets.

scholarly journals Somatic-embryogenesis derived leaf-rust-tolerant clones of arabica coffee to deal with climate change

2020 ◽  
Vol 142 ◽  
pp. 04004
Author(s):  
Rina Arimarsetiowati ◽  
Erwin Prastowo
Keyword(s):  
Somatic Embryogenesis ◽  
Leaf Rust ◽  
Leaf Explants ◽  
Germination Percentage ◽  
Growth Patterns ◽  
Embryo Germination ◽  
Essential Point ◽  
Plant Materials ◽  
Direct Embryogenesis ◽  
Planting Materials

The high-altitude coffee growing, Arabica, is likely subject to the global warming effect as they are prone to leaf-rust attacks at a higher temperature. It supplied 70% of world coffee production for its popularity concerning its delicacy and aromatic flavor. Utilization of, genetically, superior planting materials, i.e. leaf-rust-tolerant Arabica, has become an essential point, as may provide the potential solution to prevent the lost production due to leaf-rust attack. Andungsari 2K (AS 2K), S795, AS1, and Sigararutang are some of the potential leaf-rust-tolerant Arabica clones in Indonesia. Vegetative propagation by somatic embryogenesis may support the availability of superior plant materials quickly. The major aim of this experiment was to study the effect of different clones on germination step after the preliminary stage of direct-embryogenesis from leaf explants with combinations of medium between auxin (2,4-D) and cytokinin (2-ip). Embryo germination stage where embryoid was transferred to the germination medium consisting of MS medium without hormones. The results revealed that the growth location and texture of callus, as well as growth patterns and colour of embryogenic callus, were significantly influenced through the different combinations of medium and clones. The clone of S795 exhibits the highest embryo germination percentage of up to 100% within 8 weeks experiment period.

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