Identification and characterization of two ammonium transporter genes in flowering Chinese cabbage (<i>Brassica campestris</i>)

Effect of ammonium and nitrate ratios (0:100, 25:75, 50:50 and 75:25) on nutritional quality of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) with 3 cultivars were studied in hydroponics. The results indicated that, with the increasing of ammonium proportion in nutrient solution, the soluble sugar content in product organ of flowering Chinese cabbage increased gradually, but Vc content decreased gradually. Enhancement of ammonium by 25% or 50% in nutrient solution increased the soluble protein content in leaf significantly, while it increased gradually in stalk with the increasing of ammonium proportion. Free amino acids in product organ of flowering Chinese cabbage were improved significantly by enhancement of ammonium in nutrient solution. Integrated nutritional quality of flowering Chinese cabbage in 25% ammonium enhancement treatment was better than others under hydroponics condition.

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Study on Autotoxicity of Aqueous Extracts from Different Parts of Flowering Chinese Cabbage

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.641-642.962 ◽

2013 ◽

Vol 641-642 ◽

pp. 962-966 ◽

Cited By ~ 1

Author(s):

Hou Cheng Liu ◽

Shi Wei Song ◽

Ri Yuan Chen ◽

Guang Wen Sun

Keyword(s):

Cell Membrane ◽

Chlorophyll Content ◽

Chinese Cabbage ◽

Brassica Campestris ◽

Cell Membrane Permeability ◽

Aqueous Extracts ◽

Leaf Chlorophyll ◽

Flowering Chinese Cabbage ◽

Stems And Leaves ◽

Different Parts

Autotoxicity of aqueous extracts from roots, stems and leaves of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) were studied by hydroponic in different concentrations (0.5g/L, 2g/L, 5g/L). The results showed that in solution with aqueous extracts from roots, stems and leaves, the growth of flowering Chinese cabbage were inhibited, leaf chlorophyll content declined, photosynthesis rate were affected, activities of SOD, POD and CAT were changed, MDA concentration increased, thus cell membrane permeability of flowering Chinese cabbage was damaged. The autotoxicity of aqueous extracts in flowering Chinese cabbage increased with the extracts concentration increasing.

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Transcriptomic analysis of the regulation of stalk development in flowering Chinese cabbage (Brassica campestris) by RNA sequencing

Scientific Reports ◽

10.1038/s41598-017-15699-6 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 4

Author(s):

Xinmin Huang ◽

Yuling Lei ◽

Hongling Guan ◽

Yanwei Hao ◽

Houcheng Liu ◽

...

Keyword(s):

Rna Sequencing ◽

Chinese Cabbage ◽

Brassica Campestris ◽

Transcriptomic Analysis ◽

Flowering Chinese Cabbage

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Genomic Organization of the S Locus: Identification and Characterization of Genes in SLG/SRK Region of S9 Haplotype of Brassica campestris (syn. rapa)

Genetics ◽

10.1093/genetics/153.1.391 ◽

1999 ◽

Vol 153 (1) ◽

pp. 391-400 ◽

Cited By ~ 7

Author(s):

Go Suzuki ◽

Naoko Kai ◽

Tamaki Hirose ◽

Kiichi Fukui ◽

Takeshi Nishio ◽

...

Keyword(s):

Brassica Campestris ◽

Protein A ◽

S Locus ◽

Cysteine Rich Protein ◽

Pollen Coat ◽

Genes Encoding ◽

Gene Rich Region ◽

Identification And Characterization ◽

S Locus Glycoprotein

Abstract In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has revealed that the S locus is highly polymorphic and contains several genes, i.e., SLG, SRK, the as-yet-unidentified pollen S gene(s), and other linked genes. In the present study, we searched for expressed sequences in a 76-kb SLG/SRK region of the S9 haplotype of Brassica campestris (syn. rapa) and identified 10 genes in addition to the four previously identified (SLG9, SRK9, SAE1, and SLL2) in this haplotype. This gene density (1 gene/5.4 kb) suggests that the S locus is embedded in a gene-rich region of the genome. The average G + C content in this region is 32.6%. An En/Spm-type transposon-like element was found downstream of SLG9. Among the genes we identified that had not previously been found to be linked to the S locus were genes encoding a small cysteine-rich protein, a J-domain protein, and an antisilencing protein (ASF1) homologue. The small cysteine-rich protein was similar to a pollen coat protein, named PCP-A1, which had previously been shown to bind SLG.

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