Portulaca oleracea extract increases lecithin:cholesterol acyltransferase and paraoxonase 1 activities and enhances reverse cholesterol transport in streptozotocin-induced diabetic rat

2014 ◽  
Vol 6 (3) ◽  
pp. 1-9 ◽  
Author(s):  
Guenzet Akila ◽  
Krouf Djamil ◽  
Berzou Saadia
Keyword(s):  
Reverse Cholesterol Transport ◽  
Portulaca Oleracea ◽  
Paraoxonase 1 ◽  
Diabetic Rat ◽  
Cholesterol Transport ◽  
Lecithin:Cholesterol Acyltransferase

Abstract 316: Paraoxonase 1 (PON1) Promotes the Cholesterol Efflux and Regulates the Reverse Cholesterol Transport (RCT)

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Souade Ikhlef ◽  
Hicham Berrougui ◽  
Abdelouahed Khalil
Keyword(s):  
Reverse Cholesterol Transport ◽  
Cholesterol Efflux ◽  
Oxidized Ldl ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Cholesterol Transport ◽  
Phospholipase Activity ◽  
Gene And Protein Expression

Paraoxonase 1 (PON1) is a High Density Lipoprotein (HDL) associated enzyme which contributes to the antioxidant and anti-inflammatory properties of HDL. It also stimulates cholesterol efflux mediated by HDL. The purpose of this study is to determine the mechanism by which PON1 regulates this antiatherogenic activity of HDL. The oxidized phospholipids hydrolysis by PON1 results in the formation of Lysophosphatidylcholine (LPC). The phospholipase PON1 activity effect on the cholesterol efflux and the gene and protein expression were determined in vitro on J774 macrophages cells and was also determined in vivo on C57/BL6 male mice. Our results show that due to its phospholipase activity, PON1 hydrolyzes oxidized LDL (oxLDL) and contributes to the formation of LPC, which stimulates cholesterol efflux from macrophages. Our results show that PON1 induced the expression of ABCA1 cholesterol transporters. Western blot as well as qPCR analysis show that PON1 stimulate PPARγ and LXRα nuclear receptors expression. Moreover, the use of ABCA1, PPARγ and LXRα inhibitors (respectively DIDS, GGPP and GW9662) affects the capacity of PON1 to stimulate cholesterol efflux, in particular by decreasing the proteins expression of interest (ABCA1, PPARγ and LXRα). Incubation of macrophages with purified LPC with macrophages induces a high expression of PPARγ-LXRα-ABCA1 pathway. Incubation of macrophages with PON1-oxLDL mixture and their injection to mice increase significantly the reverse cholesterol transport. Our results show that tPON1 stimulate both cholesterol efflux and RCT. via the regulation of the PPARγ-LXRα-ABCA1 pathway. This effect is attributed to the phospholipase activity of PON1 which mediates the hydrolysis of ox-LDL to form LPC.

Paraoxonase-1 activities in individuals with different HDL circulating levels: Implication in reverse cholesterol transport and early vascular damage

2019 ◽  
Vol 285 ◽  
pp. 64-70 ◽  
Author(s):  
Carlo Cervellati ◽  
Giovanni Battista Vigna ◽  
Alessandro Trentini ◽  
Juana M. Sanz ◽  
Francesca Zimetti ◽  
...  
Keyword(s):  
Reverse Cholesterol Transport ◽  
Vascular Damage ◽  
Paraoxonase 1 ◽  
Cholesterol Transport ◽  
Circulating Levels

Effect of sardine proteins on hyperglycaemia, hyperlipidaemia and lecithin:cholesterol acyltransferase activity, in high-fat diet-induced type 2 diabetic rats

2015 ◽  
Vol 115 (1) ◽  
pp. 6-13 ◽  
Author(s):  
Nora Benaicheta ◽  
Fatima Z. Labbaci ◽  
Malika Bouchenak ◽  
Farida O. Boukortt
Keyword(s):  
Insulin Sensitivity ◽  
High Fat Diet ◽  
Reverse Cholesterol Transport ◽  
Serum Insulin ◽  
Diabetic Rats ◽  
Cholesterol Transport ◽  
Lcat Activity ◽  
Lecithin:Cholesterol Acyltransferase ◽  
High Fat

AbstractType 2 diabetes (T2D) is a major risk factor of CVD. The effects of purified sardine proteins (SP) were examined on glycaemia, insulin sensitivity and reverse cholesterol transport in T2D rats. Rats fed a high-fat diet (HFD) for 5 weeks, and injected with a low dose of streptozotocin, were used. The diabetic rats were divided into four groups, and they were fed casein (CAS) or SP combined with 30 or 5 % lipids, for 4 weeks. HFD-induced hyperglycaemia, insulin resistance and hyperlipidaemia in rats fed HFD, regardless of the consumed protein. In contrast, these parameters lowered in rats fed SP combined with 5 or 30 % lipids, and serum insulin values reduced in SP v. CAS. HFD significantly increased total cholesterol and TAG concentrations in the liver and serum, whereas these parameters decreased with SP, regardless of lipid intake. Faecal cholesterol excretion was higher with SP v. CAS, combined with 30 or 5 % lipids. Lecithin:cholesterol acyltransferase (LCAT) activity and HDL3-phospholipids (PL) were higher in CAS-HF than in CAS, whereas HDL2-cholesteryl esters (CE) were lower. Otherwise, LCAT activity and HDL2-CE were higher in the SP group than in the CAS group, whereas HDL3-PL and HDL3-unesterified cholesterol were lower. Moreover, LCAT activity lowered in the SP-HF group than in the CAS-HF group, when HDL2-CE was higher. In conclusion, these results indicate the potential effects of SP to improve glycaemia, insulin sensitivity and reverse cholesterol transport, in T2D rats.

scholarly journals Human paraoxonase 1 overexpression in mice stimulates HDL cholesterol efflux and reverse cholesterol transport

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173385 ◽  
Author(s):  
Souade Ikhlef ◽  
Hicham Berrougui ◽  
Olivier Kamtchueng Simo ◽  
Echarki Zerif ◽  
Abdelouahed Khalil
Keyword(s):  
Reverse Cholesterol Transport ◽  
Cholesterol Efflux ◽  
Hdl Cholesterol ◽  
Paraoxonase 1 ◽  
Cholesterol Transport

Ajuga ivaaqueous extract improves reverse cholesterol transport in streptozotocin-induced diabetic rat

2012 ◽  
Vol 64 (8) ◽  
pp. 1188-1194 ◽  
Author(s):  
Douja Taleb-Senouci ◽  
Marie A. Lacaille-Dubois ◽  
Malika Bouchenak
Keyword(s):  
Reverse Cholesterol Transport ◽  
Diabetic Rat ◽  
Cholesterol Transport
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