scholarly journals The available SRL3 deletion strain of Saccharomyces cerevisiae contains a truncation of DNA damage tolerance protein Mms2: Implications for Srl3 and Mms2 functions

10.5580/42c ◽  
2010 ◽  
Vol 8 (1) ◽  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Damage ◽  
Damage Tolerance ◽  
Deletion Strain ◽  
Dna Damage Tolerance

DNA Damage Tolerance in the Yeast Saccharomyces cerevisiae

2021 ◽  
Vol 57 (4) ◽  
pp. 379-389
Author(s):  
E. A. Alekseeva ◽  
V. G. Korolev
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Damage ◽  
Damage Tolerance ◽  
Dna Damage Tolerance ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals Participation of the HIM1 gene of yeast Saccharomyces cerevisiae in the error-free branch of post-replicative repair and role Polη in him1-dependent mutagenesis

2020 ◽  
Author(s):  
E. A. Alekseeva ◽  
T. A. Evstyukhina ◽  
V. T. Peshekhonov ◽  
V. G. Korolev
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Damage ◽  
Damage Tolerance ◽  
Dna Damage Tolerance ◽  
Uv Mutagenesis ◽  
Gene Encoding ◽  
Yeast Saccharomyces Cerevisiae ◽  
Recombination Repair ◽  
Repair Pathways

Abstract In eukaryotes, DNA damage tolerance (DDT) is determined by two repair pathways, homologous repair recombination (HRR) and a pathway controlled by the RAD6-epistatic group of genes. Monoubiquitylation of PCNA mediates an error-prone pathway, whereas polyubiquitylation stimulates an error-free pathway. The error-free pathway involves components of recombination repair; however, the factors that act in this pathway remain largely unknown. Here, we report that the HIM1 gene participates in error-free DDT. Notably, inactivation RAD30 gene encoding Polη completely suppresses him1-dependent UV mutagenesis. Furthermore, data obtained show a significant role of Polη in him1-dependent mutagenesis, especially at non-bipyrimidine sites (NBP sites). We demonstrate that him1 mutation significantly reduces the efficiency of the induction expression of RNR genes after UV irradiation. Besides, this paper presents evidence that significant increase in the dNTP levels suppress him1-dependent mutagenesis. Our findings show that Polη responsible for him1-dependent mutagenesis.

scholarly journals DNA Damage Tolerance Pathway Choice Through Uls1 Modulation of Srs2 SUMOylation in Saccharomyces cerevisiae

Genetics ◽  
2017 ◽  
Vol 206 (1) ◽  
pp. 513-525 ◽  
Author(s):  
Karol Kramarz ◽  
Seweryn Mucha ◽  
Ireneusz Litwin ◽  
Anna Barg-Wojas ◽  
Robert Wysocki ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Damage ◽  
Damage Tolerance ◽  
Dna Damage Tolerance ◽  
Pathway Choice

scholarly journals The Chromatin-binding Protein Spn1 contributes to Genome Instability in Saccharomyces cerevisiae

2018 ◽  
Author(s):  
Alison K. Thurston ◽  
Catherine A. Radebaugh ◽  
Adam R. Almeida ◽  
Juan Lucas Argueso ◽  
Laurie A. Stargell
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Dna Damage ◽  
Genome Stability ◽  
Damage Tolerance ◽  
Genome Instability ◽  
Chromatin Binding ◽  
Dna Damage Tolerance ◽  
Template Switch

AbstractCells expend a large amount of energy to maintain their DNA sequence. DNA repair pathways, cell cycle checkpoint activation, proofreading polymerases, and chromatin structure are ways in which the cell minimizes changes to the genome. During replication, the DNA damage tolerance pathway allows the replication forks to bypass damage on the template strand. This avoids prolonged replication fork stalling, which can contribute to genome instability. The DNA damage tolerance pathway includes two sub-pathways: translesion synthesis and template switch. Post-translational modification of PCNA and the histone tails, cell cycle phase, and local DNA structure have all been shown to influence sub-pathway choice. Chromatin architecture contributes to maintaining genome stability by providing physical protection of the DNA and by regulating DNA processing pathways. As such, chromatin-binding factors have been implicated in maintaining genome stability. Using Saccharomyces cerevisiae, we examined the role of Spn1, a chromatin binding and transcription elongation factor, in DNA damage tolerance. Expression of a mutant allele of SPN1 results in increased resistance to the DNA damaging agent methyl methanesulfonate, lower spontaneous and damage-induced mutation rates, along with increased chronological lifespan. We attribute these effects to an increased usage of the template switch branch of the DNA damage tolerance pathway in the spn1 strain. This provides evidence for a role of wild type Spn1 in promoting genome instability, as well as having ties to overcoming replication stress and contributing to chronological aging.

Error-free DNA-damage tolerance in Saccharomyces cerevisiae

2015 ◽  
Vol 764 ◽  
pp. 43-50 ◽  
Author(s):  
Xin Xu ◽  
Susan Blackwell ◽  
Aiyang Lin ◽  
Fangfang Li ◽  
Zhoushuai Qin ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Damage ◽  
Damage Tolerance ◽  
Dna Damage Tolerance ◽  
Free Dna

scholarly journals Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance

DNA Repair ◽  
2008 ◽  
Vol 7 (9) ◽  
pp. 1455-1470 ◽  
Author(s):  
Sanjay D'Souza ◽  
Lauren S. Waters ◽  
Graham C. Walker
Keyword(s):  
Dna Damage ◽  
Damage Tolerance ◽  
Dna Damage Tolerance ◽  
Conserved Motifs ◽  
C Terminus

DNA Topoisomerases in DNA Repair and DNA Damage Tolerance

1998 ◽  
pp. 517-537 ◽  
Author(s):  
John L. Nitiss
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Damage Tolerance ◽  
Dna Damage Tolerance ◽  
Dna Topoisomerases
Sign in / Sign up

Export Citation Format

Share Document