scholarly journals The uptake and accumulation of phosphorous and nitrates and the activity of nitrate reductase in cucumber seedlings treated with PbCl2 or CdCl2

2014 ◽  
Vol 57 (3) ◽  
pp. 349-359 ◽  
Author(s):  
Marek Burzyński
Keyword(s):  
Heavy Metals ◽  
Nitrate Reductase ◽  
Cucumis Sativus ◽  
Nutrient Medium ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Reductase Activity ◽  
Control Level ◽  
Lead And Cadmium ◽  
Phosphorous Uptake

Treatment of 4-day-old cucumber (<em>Cucumis sativus</em> L.) seedlings with PbCl<sub>2</sub> or CdCl<sub>2</sub> caused a significant increase in the accumulation of heavy metals by the plants, especially in the roots. The accumulated Pb initially enhanced the uptake of phosphorous after the plants had been transferred to a nutrient medium (6, 24 hrs), but after only 48 Ins the uptake had dropped to below control level. The plants treated with Cd exhibited a constant decreased phosphorous uptake level. The accumulated lead and cadmium also inhibited nitrate uptake and the activity of nitrate reductase. It is suggested that the reason for the decreased nitrate reductase activity lay rather in the lower nitrate uptake than in a direct effect of the heavy metals on the enzyme.

Induction of nitrate reductase activity in red spruce needles by NO2 and HNO3 vapor

1989 ◽  
Vol 19 (7) ◽  
pp. 889-896 ◽  
Author(s):  
Richard J. Norby ◽  
Yohan Weerasuriya ◽  
Paul J. Hanson
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Forest Decline ◽  
Reductase Activity ◽  
Control Level ◽  
Red Spruce ◽  
Acid Vapor ◽  
Unexposed Control ◽  
Acid Mist ◽  
Spruce Needles

The induction of the enzyme nitrate reductase in needles may be a prerequisite for the assimilation of foliar-absorbed nitrogen oxide pollutants by red spruce (Picearubens Sarg.) trees. To test for induction of nitrate reductase, 1-year-old red spruce seedlings were exposed to NO2, HNO3 vapor, or acid mist containing nitrate, and the activity of nitrate reductase in needles was measured. One day after exposure to NO2 (75 nL•L−1) began, nitrate reductase activity was three times greater than that of unexposed control plants. One day after exposure ended, the nitrate reductase activity returned to the control level. Older red spruce seedlings that had been excavated from a spruce–fir stand exhibited a similar pattern of response, but the level of nitrate reductase activity was much lower than that of the 1-year-old seedlings. Nitric acid vapor (75 nL•L−1) also induced nitrate reductase in red spruce needles, and the pattern of response was similar to that with NO2, except that the nitrate reductase activity did not return to control levels until 2 days after exposure ended. Exposure of seedlings to acid mist containing nitrate (pH 3.5 and 5.0) did not result in a change in nitrate reductase activity. These results indicate that red spruce is capable of assimilating NO2 and HNO3 vapor and that hypotheses of forest decline based on foliar assimilation of pollutant nitrogen oxides are tenable.

scholarly journals Effects of Nitrite, Chlorate, and Chlorite on Nitrate Uptake and Nitrate Reductase Activity

1992 ◽  
Vol 100 (2) ◽  
pp. 644-650 ◽  
Author(s):  
M. Yaeesh Siddiqi ◽  
Bryan J. King ◽  
Anthony D. M. Glass
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Reductase Activity

scholarly journals Induction of nitrate uptake and nitrate reductase activity in trembling aspen and lodgepole pine

1998 ◽  
Vol 21 (10) ◽  
pp. 1039-1046 ◽  
Author(s):  
X. Min ◽  
M. Y. Siddiqi ◽  
R. D. Guy ◽  
A. D. M. Glass ◽  
H. J. Kronzucker
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Lodgepole Pine ◽  
Reductase Activity ◽  
Trembling Aspen

scholarly journals Attenuation of Mercury Phytotoxicity with a High Nutritional Level of Nitrate in Alfalfa Plants Grown Hydroponically

2021 ◽  
Author(s):  
Sandra Carrasco-Gil ◽  
Cristina Ortega-Villasante ◽  
Juan Sobrino-Plata ◽  
Ángel Barón-Sola ◽  
Rocío Millán ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Lipid Peroxidation ◽  
Nitrate Reductase ◽  
Culture System ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Hydroponic Culture ◽  
Physiological Alterations ◽  
Different Levels

Abstract Background and aims Mercury (Hg) is one of the most dangerous pollutant heavy metals to the environment, which causes several toxic effects in plants upon accumulation, such as induction of oxidative stress. Nitrogen (N) is one of the most limiting macronutrient for plants, being mostly assimilated as NO3─ which is reduced to NO2─ by the enzyme nitrate reductase (NR), prior its reduction to NH4+.Methods We studied the physiological alterations caused by Hg (0, 6 and 30 µM) in alfalfa plants grown at two different levels of NO3─: low, (2 mM; LN), and high (12 mM; HN) for one week using a semi-hydroponic culture system.Results Several parameters of oxidative stress such as lipid peroxidation, chlorophyll content, biothiol concentration and, ascorbate peroxidase (APX) and glutathione reductase (GR) activity showed that HN plants were less affected by Hg. Nitrate reductase activity and NO3─ concentration was altered under Hg stress, with lower impact in plants nourished with high NO3─. Conclusions Our results highlight the importance of the nitrogen nutritional status to improve tolerance to toxic metals like Hg.

Sign in / Sign up

Export Citation Format

Share Document