scholarly journals The degradation of potato virus M (PVM) particles in plant cells

2014 ◽  
Vol 59 (1-4) ◽  
pp. 87-97
Author(s):  
Anna Rudzińska-Langwald
Keyword(s):  
Potato Virus ◽  
Close Contact ◽  
Plant Cells ◽  
Central Vacuole ◽  
Virus Particles ◽  
Lycopersicon Chilense ◽  
Solanum Rostratum ◽  
Parenchyma Cells ◽  
Large Central Vacuole ◽  
Potato Virus M

Degradation of potato virus M particles was observed in the cells of <i>Solanum tuberosum</i>, <i>Solanum rostratum</i>, <i>Lycopersicon esculentum</i> and <i>Lycopersicon chilense</i> plants infected with this virus. PVM particles found in the cytoplasm of infected parenchyma cells grouped together in the form of inclusions, often found near the tonoplast. The ends of the virus particles and the tonoplast came into close contact. Cytoplasmic protrusions containing PVM particles, reaching into vacuoles were formed in those places. In addition to a large central vacuole, small vacuoles were observed in cells containing PVM particles. Various stages of degradation of cytoplasmic protrusions were observed both in the large and small vacuoles.

scholarly journals Cytological changes in phloem parenchyma cells of Solanum rostratum (Dunal.) related to the replication of potato virus M (PVM)

2014 ◽  
Vol 59 (1-4) ◽  
pp. 45-53 ◽  
Author(s):  
Anna Rudzińska-Langwald
Keyword(s):  
Endoplasmic Reticulum ◽  
Potato Virus ◽  
Coated Particles ◽  
Virus Particles ◽  
Phloem Parenchyma ◽  
Solanum Rostratum ◽  
Parenchyma Cells ◽  
Potato Virus M ◽  
Cytological Changes

The first cytological symptom of infection of phloem parenchyma cells by potato virus M is the formation of clusters of endoplasmic reticulum cisterns in a cytoplasm containing numerous ribosomes. Randomly distributed PVM particles are found in the vicinity of the cisterns. As the infection progresses, inclusions made up of regularly arranged particles of PVM are formed.The cytoplasm of the cells becomes electron transparent because the ER cisterns disappear. Masses of homogenous substances containing single PVM particles appear. There are two types of deposits in the inclusions containing PVM virus particles - additionally coated particles and tubules.

Detection of potato virus M and potato virus S on test plants

1976 ◽  
Vol 19 (2) ◽  
pp. 131-139 ◽  
Author(s):  
A. Kowalska ◽  
M. Waś
Keyword(s):  
Potato Virus ◽  
Potato Virus S ◽  
Potato Virus M ◽  
Test Plants

scholarly journals Interaction of Theiler’s Virus with Intermediate Filaments of Infected Cells

1998 ◽  
Vol 72 (12) ◽  
pp. 9553-9560 ◽  
Author(s):  
Patrick Nédellec ◽  
Patrick Vicart ◽  
Christine Laurent-Winter ◽  
Cécile Martinat ◽  
Marie-Christine Prévost ◽  
...  
Keyword(s):  
Intermediate Filaments ◽  
Intermediate Filament ◽  
Close Contact ◽  
Theiler's Virus ◽  
Virus Particles ◽  
Host Cell Proteins ◽  
Encephalomyelitis Virus ◽  
Infected Cells ◽  
Main Components ◽  
Filament Network

ABSTRACT Theiler’s murine encephalomyelitis virus is a neurotropic murine picornavirus which replicates permissively and causes a cytopathic effect in the BHK-21 cell line. We examined the interactions between the GDVII and DA strains of Theiler’s virus and BHK-21 host cell proteins in a virus overlay assay. We observed binding of the virions to two proteins of approximately 60 kDa. These proteins were microsequenced and identified as desmin and vimentin, two main components of the intermediate filament network. The association between desmin or vimentin and virions was demonstrated by immunoprecipitation. Anti-desmin and anti-vimentin monoclonal antibodies precipitated GDVII or DA virions from extracts of infected BHK-21 cells. The intracellular distributions of virions and of the desmin and vimentin intermediate filaments of BHK-21 cells were investigated by two-color immunofluorescence confocal microscopy. Following infection, the intermediate filament network was rearranged into a shell-like structure which surrounded a viral inclusion. Finally, close contact between GDVII virus particles and 10-nm intermediate filaments was observed by electron microscopy.

Ultrastructure of potato infected with potato virus M

Virology ◽  
1970 ◽  
Vol 42 (1) ◽  
pp. 238-242 ◽  
Author(s):  
J.C. Tu ◽  
C. Hiruki
Keyword(s):  
Potato Virus ◽  
Potato Virus M

Ultrastructure of acridone alkaloid idioblasts in roots and cell cultures of Ruta graveolens

1986 ◽  
Vol 64 (6) ◽  
pp. 1089-1096 ◽  
Author(s):  
U. Eilert ◽  
B. Wolters ◽  
F. Constabel
Keyword(s):  
Cell Cultures ◽  
Histological Analysis ◽  
Suspension Cultures ◽  
Central Vacuole ◽  
Ruta Graveolens ◽  
Fungal Elicitors ◽  
Parenchyma Cells ◽  
Acridone Alkaloids ◽  
Acridone Alkaloid

Histological analysis of Ruta graveolens L. roots and in vitro grown cell suspensions revealed idioblasts with vacuoles containing clusters of droplets thought to be the storage compartment of acridone alkaloids. These idioblasts contained numerous vacuoles of varying sizes rather than the large, single, central vacuole characteristic of most adjacent parenchyma cells. The structure of idioblasts in roots and suspension cultures was identical. Treatment of suspension cultures with fungal elicitors known to increase alkaloid accumulation greatly did not affect the structure of idioblasts.

scholarly journals Detection and Quantification of Potato virus Y Genomes in Single Aphid Stylets

Plant Disease ◽  
2019 ◽  
Vol 103 (9) ◽  
pp. 2315-2321 ◽  
Author(s):  
M. Khelifa
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Detection Method ◽  
Specific Method ◽  
Virus Particles ◽  
Reverse Transcription Pcr ◽  
Pcr Method ◽  
The One ◽  
Higher Sensitivity ◽  
Detection And Quantification

Typically, the detection of a plant virus within its vector is carried out on the entire insect body. This process can be a possible source of confusion in the quantification of transmissible virus particles for styletborne viruses such as Potato virus Y (PVY), since the transmissible virus fraction is the one only retained in the aphid vector’s mouthparts. The objective of this study was to develop and validate the quantitative PCR method for the detection and quantification of PVY in the vector’s stylet. Using a specific method based on TaqMan chemistry with higher sensitivity than conventional reverse transcription PCR, this study reveals that a significant amount of the virus is enclosed within the dissected stylets of Myzus persicae. Because this quantification only concerns the portion of the virus attached to the stylets, uniformity was observed in the recorded numbers of virus targets. This novel assay is applicable to several PVY strains as a rapid and sensitive detection method for use in PVY research and offers a convenient tool for deciphering the mechanism of Potyvirus acquisition.

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