scholarly journals Uptake of fluorescent-labeled BSA into root cells: endocytosis?

2014 ◽  
Vol 66 (3-4) ◽  
pp. 319-324 ◽  
Author(s):  
A. Kaźmierczak ◽  
J. Maszewski
Keyword(s):  
Zea Mays ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cytochalasin B ◽  
Fluorescein Isothiocyanate ◽  
Cell Type ◽  
Root Cells ◽  
Root Zones ◽  
Species Specific

Incorporation of rhodamine- and fluorescein-isothiocyanate labeled bovine serum albumin (BSA-TRITC, BSA-FITC) was examined in different root zones of the 3-day-old seedlings in <em>Melandrium noctiflorum</em>, <em>Allium cepa</em> and <em>Zea mays</em>. The uptake of fluorescent-labeled BSA was found: (1) species-specific, (2) cell-type dependent, and (3) cytochalasin B-sensitive. The characteristic punctute distribution of vesicles within the cytoplasm suggests the internalization of labeled proteins by endocytosis.

scholarly journals Self-assembled bovine serum albumin nanoparticles as pesticide delivery vectors for controlling trunk-boring pests

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Chenyu Su ◽  
Shanshan Liu ◽  
Shenghan Cao ◽  
Shuyan Yin ◽  
Chenggang Zhou ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Self Assembly ◽  
Bovine Serum ◽  
Fluorescein Isothiocyanate ◽  
Effective Control ◽  
Transport Efficiency ◽  
Dead Trees ◽  
Self Assembled ◽  
And Control

Abstract Background Trunk-boring pests (TBPs) are an important type of forest pest, TBPs not only feed on the branches and trunks of trees, but also spread quarantine diseases in forests. However, because the larvae of TBPs live inside the trunk and are well concealed, prevention and control are difficult. The lack of effective control methods leads to the death of many trees in forests. In this study, a novel nanopesticide featuring high bioactivity and slow-release properties was developed to control TBPs. Thiacloprid (THI), which is commonly used to control Coleoptera species, was used as a model pesticide. Results The oleophobic properties of bovine serum albumin (BSA) were exploited to encapsulate the hydrophobic pesticide THI by self-assembly, and the size of the obtained nanoparticles, THI@BSA·NPs, was approximately 23 nm. The loading efficiency reached 70.4%, and THI@BSA·NPs could be released continuously for over 15 days, with the cumulative release reaching 93.5%. The fluorescein isothiocyanate (FITC)-labeled nanoparticles were evenly distributed in the digestive tract and body surface of a typical TBPs, M. alternatus, and the stomach and contact toxicities increased by 33.7% and 25.9%, respectively, compared with those of free THI. Furthermore, the results showed that the transport efficiency of THI@BSA·NPs was highest at a concentration of 50 μg/mL, and the THI@BSA·NPs content in the trunk, from to lower to higher layers, was 8.8, 8.2, 7.6, and 5.8 μg/g. At the same time, THI@BSA·NPs also exhibited high transport efficiency in dead trees. Conclusion The transport efficiency and toxicity of the active ingredients are the key factors for the control of TBPs. This work provided idea for the application of biological delivery system encapsulated hydrophobic pesticides. The novel self-assembled THI@BSA·NPs have promising potential for sustainable control of TBPs.

scholarly journals Histochemical demonstration of estrogen and progesterone binding in endometriotic tissue and in uterine endometrium: A comparative study.

1985 ◽  
Vol 33 (2) ◽  
pp. 155-161 ◽  
Author(s):  
A Bergqvist ◽  
S Jeppsson ◽  
O Ljungberg
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Specific Binding ◽  
Relative Proportion ◽  
Fluorescein Isothiocyanate ◽  
Histochemical Demonstration ◽  
Endometrial Tissue ◽  
Menstrual Phase ◽  
Uterine Endometrium

Estrogen and progesterone binding to endometriotic and endometrial tissue was studied histochemically using estradiol and progesterone fluorochrome derivatives (E2-bovine serum albumin-fluorescein isothiocyanate and progesterone-bovine serum albumin-tetramethylrhodamine isothiocyanate). Thirty endometriotic samples from 21 women were studied, together with endometrial specimens obtained simultaneously from 14 of the women. In 77% of the endometriotic samples binding of the estrogen conjugate was indicated by specific fluorescence in more than half of the epithelial cell population, and in 20% in less than half. The corresponding figures for the progesterone conjugate binding were 75 and 18%, respectively. Blocking studies indicated a reasonable degree of ligand specificity. In endometrial tissue the corresponding figures were 64 and 29%, respectively, for binding of the estrogen conjugate and 54 and 38%, respectively, for binding of the progesterone conjugate. In 7 of 13 cases where evaluable samples of both tissues had been obtained, the relative proportion of fluorescent cells, with either reagent, was similar in the two tissue types. Our results suggest that the cytoplasm of epithelial cells in endometriotic tissue and in uterine endometrium contains specific binding sites for both estrogen and progesterone. The binding pattern of the two conjugates in endometriotic tissue was unrelated to the menstrual phase.

scholarly journals Self-Assembled Bovine Serum Albumin Nanoparticles as Pesticide Delivery Vectors for Controlling Trunk-Boring Pests

2020 ◽  
Author(s):  
Chenyu Su ◽  
Shanshan Liu ◽  
Shenghan Cao ◽  
Shuyan Yin ◽  
Chenggang Zhou ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Self Assembly ◽  
Bovine Serum ◽  
Fluorescein Isothiocyanate ◽  
Effective Control ◽  
Transport Efficiency ◽  
Dead Trees ◽  
Self Assembled ◽  
And Control

Abstract Trunk-boring pests (TBPs) are an important type of forest pest, TBPs not only feed on the branches and trunks of trees, but also spread quarantine diseases in forests. However, because the larvae of TBPs live inside the trunk and are well concealed, prevention and control are difficult. The lack of effective control methods leads to the death of many trees in forests. In this study, a novel nanopesticide featuring high bioactivity and slow-release properties was developed to control TBPs. Thiacloprid (THI), which is commonly used to control Coleoptera species, was used as a model pesticide. The oleophobic properties of bovine serum albumin (BSA) were exploited to encapsulate the hydrophobic pesticide THI by self-assembly, and the size of the obtained nanoparticles, THI@BSA·NPs, was approximately 23 nm. The loading efficiency reached 70.4%, and THI@BSA·NPs could be released continuously for over 15 d, with the cumulative release reaching 93.5%. The fluorescein isothiocyanate (FITC)-labeled nanoparticles were evenly distributed in the digestive tract and body surface of a typical TBP, M. alternatus , and the stomach and contact toxicities increased by 33.7% and 25.9%,respectively, compared with those of free THI. Furthermore, the results showed that the transport efficiency of THI@BSA·NPs was highest at a concentration of 50 μg/mL, and the THI@BSA·NPs content in the trunk, from to lower to higher layers, was 8.8, 8.2, 7.6, and 5.8 μg/g. At the same time, THI@BSA·NPs also exhibited high transport efficiency in dead trees. The results suggested that these novel self-assembled THI@BSA·NPs have promising potential for sustainable control of TBPs.

scholarly journals A simple fluorescent method for simultaneous determination of aortic permeability to horseradish peroxidase and bovine serum albumin.

1981 ◽  
Vol 29 (12) ◽  
pp. 1405-1410 ◽  
Author(s):  
T M Hollis ◽  
M E Katora ◽  
J Montini
Keyword(s):  
Bovine Serum Albumin ◽  
Horseradish Peroxidase ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fluorescent Protein ◽  
Fluorescein Isothiocyanate ◽  
Mean Values ◽  
Protein Tracers ◽  
Net Uptake ◽  
Male Wistar Rats

Differences in regional aortic net uptake of bovine serum albumin (BSA) and horseradish peroxidase (HP) have been examined by means of conjugation of these molecules to the fluorescent protein tracers fluorescein isothiocyanate (FITC) and lissamine rhodamine B (RB200). Using male Wistar rats, uptake of FITC-BSA under steady state conditions in the ascending aorta and aortic arch (14 +/- 2 micrograms/mg aorta) is significantly higher (p less than 0.05) than that of either the upper, middle, or lower third of the thoracic aorta (10 +/- 1 mu, 9 +/- 1 mu, and 8 +/- 1 micrograms/mg, respectively). No regional variation in net uptake of RB200-HP was observed in these same aortic regions, with respective mean values (+/- SE) being 69 +/- 2, 69 +/- 2, 68 +/- 4, and 68 +/- 4 micrograms/mg. Examination of fluorescence photomicrographs indicate that FITC-BSA is localized along the collagen-elastin bands, while the RB200-HP is found between these bands. Differences between FITC-BSA and RB200-HP uptake and deposition reflect such things as differences in uptake rates as influenced by initial concentrations, permeability coefficients, as well as possible differences in molecular charge and affinity. The results indicate that the fluorometric procedures described in this investigation are simple, sensitive, and quantitative, and suitable for simultaneous measurement of aortic uptake of molecules having different molecular sizes as well as for the intraaortic localization of these substances.

Application of Hollow Microneedle for Transdermal Delivery of Bovine Serum Albumin-Fluorescein Isothiocyanate Conjugate

2011 ◽  
Vol 338 ◽  
pp. 365-368 ◽  
Author(s):  
Maleenart Petchsangsai ◽  
Nanthida Wonglertnirant ◽  
Theerasak Rojanarata ◽  
Praneet Opanasopit ◽  
Tanasait Ngawhirunpat
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Transdermal Delivery ◽  
Bovine Serum ◽  
Fluorescein Isothiocyanate ◽  
Molecular Compound ◽  
Lower Epidermis ◽  
Hollow Microneedle ◽  
Superficial Dermis ◽  
Neonatal Pig

The objective of the present study was to use hollow microneedles for enhancing the transdermal delivery of Bovine Serum Albumin (MW~66,000 Da)-Fluorescein Isothiocyanate (BSA-FITC) conjugate, a hydrophilic large molecular compound. BSA-FITC was successfully loaded into the lower epidermis as well as the superficial dermis of the skin of neonatal pig by a hollow microneedle. The higher the volume of BSA-FITC solution injected, the higher the amount and the faster the BSA-FITC release rate from skin. These release profiles of BSA-FITC solution were expressed by Fick’s law of diffusion. These results suggest the utilization of hollow microneedle to enhance transdermal delivery of protein and provide useful information for designing an effective hollow microneedle system.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

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