scholarly journals Genetic differentiation of Allium sibiricum L. populations in Poland based on their morphological and molecular markers

2019 ◽  
Vol 88 (1) ◽  
Author(s):  
Jacek Urbaniak ◽  
Paweł Kwiatkowski ◽  
Bartosz Kozak

<em>Allium sibiricum</em> is one of the rarest plant species in the Sudetes and Carpathians. Inter simple sequence repeat DNA (ISSR) and morphological analyses were conducted to study the biogeographical relationships between geographically disjunctive populations of <em>A. sibiricum</em> in the Carpathians and Sudetes. The results clearly differentiated the Carpathian and Sudetes populations, but also showed a relatively high level of genetic similarity in specimens within certain groups of the Sudetes and Carpathian populations. The plants collected in the Karkonosze probably belong to a mountain form of <em>A. schoenoprasum</em> morphotype C which inhabits natural sites there. In contrast, the study found morphologically and genetically different plants inhabiting Pilsko Mt in the Carpathians. The plants from the Carpathians were present in scattered sites probably due to the activity of Vlach shepherds and the formation of large pastures. The species enlarged its local range due to this type of anthropopressure and likely hybridized with the cultivated <em>Allium</em> plants introduced by the shepherds. This may indicate that the populations on Pilsko Mt are of a partly anthropogenic character.

2016 ◽  
Vol 52 (9) ◽  
pp. 963-968 ◽  
Author(s):  
G. E. Sulimova ◽  
V. N. Voronkova ◽  
A. V. Perchun ◽  
I. F. Gorlov ◽  
A. V. Randelin ◽  
...  

2008 ◽  
Vol 88 (2) ◽  
pp. 313-322 ◽  
Author(s):  
S. C. Debnath ◽  
S. Khanizadeh ◽  
A. R. Jamieson ◽  
C. Kempler

The goal of this study was to determine the level of genetic diversity and relatedness among 16 strawberry (Fragaria H ananassa Duch.) cultivars and 11 breeding lines developed in Canada, using Inter Simple Sequence Repeat (ISSR) markers. Seventeen primers generated 225 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 63 to 77% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.4% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among strawberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in current strawberry breeding programs. Key words: Fragaria × ananassa, DNA fingerprinting, multivariate analysis, breeding, genetic similarity


2003 ◽  
Vol 3 (1) ◽  
pp. 88-90 ◽  
Author(s):  
T. Sugaya ◽  
H. Yoshimaru ◽  
T. Takeuchi ◽  
M. Katsuta ◽  
K. Fujimoto ◽  
...  

2017 ◽  
Vol 16 (3) ◽  
pp. 249-259 ◽  
Author(s):  
Omar Idrissi ◽  
Angela R. Piergiovanni ◽  
Faruk Toklu ◽  
Chafika Houasli ◽  
Sripada M. Udupa ◽  
...  

AbstractThe Mediterranean region has a rich history of domestication and cultivation of lentil (Lens culinarisMedik.). Landraces have been grown and repeatedly selected by local farmers under different agro-environments. Characterization of molecular variation and genetic differentiation helps to ensure enhanced valorization, conservation and use of these genetic resources. Nineteen Simple Sequence Repeat DNA markers were used for molecular variance analysis (AMOVA) and population structure assessment underlying 74 lentil landraces from four Mediterranean countries: Morocco, Italy, Greece and Turkey. Based on AMOVA, presence of population structure and genetic differentiation at different levels were evidenced. Genetic diversity among Turkish landraces was higher than that of other countries. These landraces were more homogeneous as shown by low genetic differentiation among individuals within each landrace. Whereas Moroccan landraces followed by Italian and Greek provenances showed higher diversity and differentiation among individuals within landraces. The wide genetic variability of these landraces could help to better adaptation to biotic and abiotic stresses. Moreover, they could provide useful alleles related to adaptive traits for breeding purposes. Based on structure analysis, we obtained indications of possible presence of two major gene pools: a northern gene pool composed of Turkish, Italian and Greek landraces, and a southern gene pool composed of Moroccan landraces. Our results could be of interest when designing future diversity studies, collection missions, conservation and core collection construction strategies on Mediterranean lentil landraces.


2020 ◽  
Vol 345 ◽  
pp. 15-25
Author(s):  
Meryem MAKKAOUI ◽  
Younes ABBAS ◽  
Salwa EL ANTRY-TAZY ◽  
Leila MEDRAOUI ◽  
Mohammed ALAMI ◽  
...  

Tetraclinis articulata (Vahl) Masters is one of Morocco's most important forest species. It is also found occasionally in Malta and Spain, showing significant adaptability to different bio-climatic conditions. However, the species is being affected by anthropogenic fragmentation, logging and neglect from authorities, which could lead to the irretrievable loss of this resource. In this study, the genetic diversity and genetic structure of ten Moroccan populations of T. articulata were assessed. Fifteen Inter-Simple Sequence Repeat (ISSR) markers were used. These generated 271 polymorphic fragments with an average of 18.06 per primer and showed 79.59% of polymorphism. The 129 individuals revealed a high level of genetic diversity (Hs = 0.221; Ht = 0.254) and 85% of genetic variation within populations. However, the genetic differentiation level was low (Gst = 0.13), which is consistent with the lack of correlation between genetic and geographic distances revealed by the Mantel test, resulting in a high level of gene flow (Nm = 3.294). Based on PCoA and neighbour-joining methods, the ten populations clustered under the effect of continental and marine climates. Compared with other conifers, the current genetic diversity and the pattern of T. articulata population structure indicate an important gene pool requiring efficient conservation strategies.


Heredity ◽  
1998 ◽  
Vol 81 (4) ◽  
pp. 422-428 ◽  
Author(s):  
Tokuko Ujino ◽  
Takayuki Kawahara ◽  
Yoshihiko Tsumura ◽  
Teruyoshi Nagamitsu ◽  
Hiroshi Yoshimaru ◽  
...  

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