scholarly journals MALDI-TOF MS Detection of Endophytic Bacteria Associated with Great Nettle (Urtica dioica L.), Grown in Algeria

2018 ◽  
Vol 67 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Souheyla Toubal ◽  
Ouahiba Bouchenak ◽  
Djillali Elhaddad ◽  
Karima Yahiaoui ◽  
Sarah Boumaza ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Vascular System ◽  
Common Species ◽  
Urtica Dioica ◽  
Biochemical Tests ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Identification Of Bacteria ◽  
The Common ◽  
Tof Ms

Any plant with a vascular system has a specific endophytic microflora. The identification of bacteria is essential in plant pathology. Although identification methods are effective, they are costly and time consuming. The purpose of this work is to isolate and to identify the different bacteria from the internal tissues of Urtica dioica L. and to study their diversity. This last is based on the different parts of the plant (stems, leaves and roots) and the harvest regions (Dellys and Tlamcen). The identification of bacteria is done by biochemical tests and confirmed by MALDI-TOF MS. Seven genus and eleven species were isolated from the Great Nettle. They belong to the genera Bacillus, Escherichia, Pantoea, Enterobacter, Staphylococcus, Enterococcus and Paenibacillus. The majority of these bacteria were isolated from Tlemcen which makes this region the richest in endophytic bacteria compared to that harvested from Dellys. The results show also that the leaves are the most diversified in endophytic bacteria. Bacillus pumilus-ME is the common species of the three parts of the plant harvested in both regions. From this work, it emerges that the Great Nettle can be settled by various endophytic bacteria which are differently distributed within the same plant harvested in different regions.

Assessment of the potential use of MALDI-TOF MS for the identification of bacteria associated with chilled vacuum-packaged lamb meat

Meat Science ◽  
2021 ◽  
pp. 108508
Author(s):  
Mohammed Altakhis ◽  
Christopher J. Pillidge ◽  
A. Mark Osborn ◽  
Peter J. Torley ◽  
Mandeep Kaur
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Lamb Meat ◽  
Identification Of Bacteria ◽  
Potential Use ◽  
Tof Ms

MALDI-TOF MS-Based Identification of Bacteria and a Survey of Fresh Vegetables with Pathogenic Bacteria in Beijing, China

2020 ◽  
pp. 100746
Author(s):  
Xiaomin Xu ◽  
Guangyang Liu ◽  
Xiaodong Huang ◽  
Lingyun Li ◽  
Huan Lin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Fresh Vegetables ◽  
Identification Of Bacteria ◽  
Beijing China ◽  
Tof Ms

Identification of Staphylococcus pseudintermedius isolates from wound cultures by MALDI-TOF MS improves accuracy of susceptibility reporting at an increase in cost

2021 ◽  
Author(s):  
Helen L. Bibby ◽  
Kristen L. Brown
Keyword(s):  
Susceptibility Testing ◽  
Biochemical Tests ◽  
Additional Time ◽  
Identification Procedure ◽  
Staphylococcus Pseudintermedius ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Before And After ◽  
Coagulase Positive Staphylococci ◽  
Tof Ms

Staphylococcus pseudintermedius can easily be mistaken for Staphylococcus aureus using phenotypic and rapid biochemical methods. We began confirming the identification of all coagulase-positive staphylococci isolated from human wound cultures at our centralized laboratory, servicing both community and inpatients, with MALDI-TOF MS instead of using phenotypic and rapid biochemical tests, and determined the prevalence of S. pseudintermedius since the change in identification procedure and at what cost. A retrospective review was performed on all wound swab cultures from which coagulase-positive staphylococci were isolated 7 months before and after the change in identification procedure. A total of 49 S. intermedius /pseudintermedius (SIP) isolates were identified including 7 isolates from 14,401 wound cultures in the before period and 42 isolates from 14,147 wound cultures in the after period. The number of SIP isolates as a proportion of isolated coagulase-positive staphylococci increased significantly from the before 7/6,351 (0.1%) to the after period 42/5,435 (0.7%) (difference 0.6% (95% CI 0.037-0.83%, p <0.0001)). Antibiotic susceptibility testing was performed in 42 isolates; none had an oxacillin MIC 1.0-2.0 μg/mL, the range in which if the isolate was misidentified as S. aureus , a very major error in susceptibility interpretation would occur. The increase in cost of the change in identification procedure was $17,558 CDN per year in our laboratory performing microbiology testing for community and acute care patients in a zone servicing nearly 1.7 million people. While we will only continue to learn more about this emerging pathogen if we make attempts to properly identify it in clinical cultures, the additional time and cost involved may be unacceptably high in some laboratories.

scholarly journals Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology

2014 ◽  
Vol 8 (09) ◽  
pp. 1081-1088 ◽  
Author(s):  
Elena De Carolis ◽  
Antonietta Vella ◽  
Luisa Vaccaro ◽  
Riccardo Torelli ◽  
Teresa Spanu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Susceptibility ◽  
Biochemical Tests ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Bacteria And Fungi ◽  
Diagnostic Microbiology ◽  
Tof Ms

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled. In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures. Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection.

scholarly journals Evaluation of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Rapid Identification of Bacteria in Processed Soybean Products

2013 ◽  
Vol 2 (3) ◽  
pp. 104 ◽  
Author(s):  
Yuko Furukawa ◽  
Mitsuru Katase ◽  
Kazunobu Tsumura
Keyword(s):  
Laser Desorption ◽  
Rapid Identification ◽  
Bacterial Cells ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Identification Of Bacteria ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently been demonstrated as a rapid and reliable method for identifying bacteria in colonies grown on culture plates. Rapid identification of food spoilage bacteria is important for ensuring the quality and safety of food. To shorten the time of analysis, several researchers have proposed the direct MALDI-TOF MS tequnics for identification of bacteria in clinical samples such as urine and positive blood cultures. In this study, processed soybean products (total 26 test samples) were initially conducted a culture enrichiment step and bacterial cells were separated from interfering components. Harvested bacterial cells were determined by MALDI-TOF MS and 16S rRNA gene sequencing method. Six processed soybean products (23%) were increased bacterial cells after culture enrichiment step and they were sucessfully obtained the accurate identification results by MALDI-TOF MS-based method without colony formation.

Rapid identification of bacteria from bioMerieux BacT/ALERT blood culture bottles by MALDI-TOF MS

2013 ◽  
Vol 70 (4) ◽  
pp. 149-155 ◽  
Author(s):  
J.D. Haigh ◽  
I.M. Green ◽  
D. Ball ◽  
M. Eydmann ◽  
M. Millar ◽  
...  
Keyword(s):  
Blood Culture ◽  
Rapid Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Identification Of Bacteria ◽  
Tof Ms

scholarly journals Sample preparation and culture condition effects on MALDI‐TOF MS identification of bacteria: A review

2021 ◽  
Author(s):  
Natalija Topić Popović ◽  
Snježana P. Kazazić ◽  
Krunoslav Bojanić ◽  
Ivančica Strunjak‐Perović ◽  
Rozelindra Čož‐Rakovac
Keyword(s):  
Sample Preparation ◽  
Culture Condition ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Identification Of Bacteria ◽  
Tof Ms

scholarly journals Application of MALDI-TOF MS for the Identification of Food Borne Bacteria

2013 ◽  
Vol 7 (1) ◽  
pp. 135-141 ◽  
Author(s):  
Melanie Pavlovic ◽  
Ingrid Huber ◽  
Regina Konrad ◽  
Ulrich Busch
Keyword(s):  
Associated Bacteria ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Current State ◽  
Food Borne ◽  
Routine Identification ◽  
Identification Of Bacteria ◽  
Cost Efficient ◽  
Tof Ms

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful tool for the routine identification of clinical isolates. MALDI-TOF MS based identification of bacteria has been shown to be more rapid, accurate and cost-efficient than conventional phenotypic techniques or molecular methods. Rapid and reliable identification of food-associated bacteria is also of crucial importance for food processing and product quality. This review is concerned with the applicability of MALDI-TOF MS for routine identification of foodborne bacteria taking the specific requirements of food microbiological laboratories and the food industry into account. The current state of knowledge including recent findings and new approaches are discussed.

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