Effect of short-term fasting on the expression of ACTH (cMC2) receptor in the adrenal glands of chicken (Gallus domesticus)

<b>Domestic hen is a full model in terms of stress and adrenal function. The main hormone produced by the hens’ adrenals is corticosterone, synthesized and secreted by stimulating the HPA axis during stress. Direct activation of adrenal activity is conditioned by ACTH, which binds to the melanocortin receptor cMC2 in adrenals. It stimulates the synthesis and release of corticosterone. One of the factors that stimulate the HPA axis activity is the starvation, to which the hen is very sensitive. The purpose of this study was to determine the ACTH receptor cMC2 expression in the hens’ adrenals during the short-term fasting and after restoring the proper level of nutrition (refeeding). The results of the experiment show that 24-hour of food deprivation is stressful for the hen, as indicated by increased concentrations of corticosterone in the adrenals and in blood plasma. Changes in cMC2R expression and level of corticosterone in the adrenals during fasting and refeeding indicate a rapid increase of HPA axis activity in response to differentiated levels of nutrition. The results of this experiment confirm the direct effect of ACTH on the avian adrenals in corticosterone release.

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Antioxidant Treatment Induces Hyperactivation of the HPA Axis by Upregulating ACTH Receptor in the Adrenal and Downregulating Glucocorticoid Receptors in the Pituitary

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/4156361 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 10

Author(s):

Jessika P. Prevatto ◽

Rafael C. Torres ◽

Bruno L. Diaz ◽

Patrícia M. R. e Silva ◽

Marco A. Martins ◽

...

Keyword(s):

Vitamin E ◽

Hpa Axis ◽

Negative Feedback ◽

Feedback Loop ◽

Glucocorticoid Receptors ◽

Plasma Corticosterone ◽

Acth Receptor ◽

Male Wistar Rats ◽

Hpa Axis Activity ◽

Nrf2 Expression

Glucocorticoid (GC) production is physiologically regulated through a negative feedback loop mediated by the GC, which appears disrupted in several pathological conditions. The inability to perform negative feedback of the hypothalamus-pituitary-adrenal (HPA) axis in several diseases is associated with an overproduction of reactive oxygen species (ROS); however, nothing is known about the effects of ROS on the functionality of the HPA axis during homeostasis. This study analyzed the putative impact of antioxidants on the HPA axis activity and GC-mediated negative feedback upon the HPA cascade. Male Wistar rats were orally treated with N-acetylcysteine (NAC) or vitamin E for 18 consecutive days. NAC-treated rats were then subjected to a daily treatment with dexamethasone, which covered the last 5 days of the antioxidant therapy. We found that NAC and vitamin E induced an increase in plasma corticosterone levels. NAC intensified MC2R and StAR expressions in the adrenal and reduced GR and MR expressions in the pituitary. NAC also prevented the dexamethasone-induced reduction in plasma corticosterone levels. Furthermore, NAC decreased HO-1 and Nrf2 expression in the pituitary. These findings show that antioxidants induce hyperactivity of the HPA axis via upregulation of MC2R expression in the adrenal and downregulation of GR and MR in the pituitary.

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