scholarly journals Usefulness of CHROMagar Candida Medium, Biochemical Methods – API ID32C and VITEK 2 Compact and Two MALDI-TOF MS Systems for Candida spp. Identification

2016 ◽  
Vol 65 (1) ◽  
pp. 111-114 ◽  
Author(s):  
Elżbieta Stefaniuk ◽  
Anna Baraniak ◽  
Monika Fortuna ◽  
Waleria Hryniewicz
Keyword(s):  
Candida Spp ◽  
Maldi Tof Ms ◽  
Biochemical Methods ◽  
Maldi Tof ◽  
Vitek 2 ◽  
Tof Ms

scholarly journals Identificación de aislamientos de Candida auris recuperados a través de la vigilancia por laboratorio en Colombia: un reto para el diagnóstico

Infectio ◽  
2020 ◽  
Vol 24 (4) ◽  
pp. 224
Author(s):  
Silvia K. Carvajal-Valencia ◽  
Diana Lizarazo ◽  
Carolina Duarte ◽  
Patricia Escandon
Keyword(s):  
Candida Auris ◽  
Candida Spp ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Vitek 2 ◽  
Bruker Daltonics ◽  
Tof Ms

Objetivo: Comparar los resultados obtenidos de diferentes sistemas de identificación de C. auris.Métodos: Análisis descriptivo con datos recopilados durante 2016-19 mediante la vigilancia nacional. Se evaluaron los resultados generados por los sistemas MicroScan, Phoenix BD, VITEK 2 y MALDI-TOF MS de instituciones hospitalarias de 843 aislamientos clínicos sospechosos de C. auris remitidos al INS y se compararon con los resultados generados de confirmación a través de MALDI- TOF MS (Bruker Daltonics) o PCR. Resultados: De los 843 aislamientos clínicos remitidos al INS, el 81,7% fueron confirmados como C. auris mediante MALDI- TOF MS o PCR en el INS y el resto, 18,3%, fueron identificados como otras especies de Candida spp. Las identificaciones correctas enviadas por los laboratorios representaron el 42,4%. MicroScan identificó C. auris principalmente como C. haemulonii, C. guilliermondii, C. albicans y C. famata; Phoenix BD, VITEK 2 y MALDI-TOF MS identificó C. auris como C. haemulonii. Discusión: Estudios señalan que C. auris exhibe una estrecha relación filogenética con C. haemulonii. Las identificaciones discrepantes pueden darse debido a que las bases de datos de los sistemas de diagnóstico son limitadas para este patógeno. Las deficiencias de los sistemas comerciales para la identificación de C. auris deben ser complementados con otros sistemas como MALDI-TOF MS o pruebas moleculares.

scholarly journals Candida auris Clinical Isolates from South Korea: Identification, Antifungal Susceptibility, and Genotyping

2019 ◽  
Vol 57 (4) ◽  
Author(s):  
Yong Jun Kwon ◽  
Jong Hee Shin ◽  
Seung A Byun ◽  
Min Ji Choi ◽  
Eun Jeong Won ◽  
...  
Keyword(s):  
Antifungal Susceptibility ◽  
Diagnostic Tools ◽  
Candida Auris ◽  
Content Type ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Fluconazole Resistant ◽  
Vitek 2 ◽  
Vitek Ms ◽  
Tof Ms

ABSTRACT Candida auris is an emerging worldwide fungal pathogen. Over the past 20 years, 61 patient isolates of C. auris (4 blood and 57 ear) have been obtained from 13 hospitals in Korea. Here, we reanalyzed those molecularly identified isolates using two matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) systems, including Biotyper and Vitek MS, followed by antifungal susceptibility testing, sequencing of the ERG11 gene, and genotyping. With a research-use-only (RUO) library, 83.6% and 93.4% of the isolates were correctly identified by Biotyper and Vitek MS, respectively. Using an in vitro diagnostic (IVD) library of Vitek MS, 96.7% of the isolates were correctly identified. Fluconazole-resistant isolates made up 62.3% of the isolates, while echinocandin- or multidrug-resistant isolates were not found. Excellent essential (within two dilutions, 96.7%) and categorical agreements (93.4%) between the Clinical and Laboratory Standards Institute (CLSI) and Vitek 2 (AST-YS07 card) methods were observed for fluconazole. Sequencing ERG11 for all 61 isolates revealed that only 3 fluconazole-resistant isolates showed the Erg11p amino acid substitution K143R. All 61 isolates showed identical multilocus sequence typing (MLST). Pulsed-field gel electrophoresis (PFGE) analyses revealed that both blood and ear isolates had the same or similar patterns. These results show that MALDI-TOF MS and Vitek 2 antifungal susceptibility systems can be reliable diagnostic tools for testing C. auris isolates from Korean hospitals. The Erg11p mutation was seldom found among Korean isolates of C. auris, and multidrug resistance was not found. Both MLST and PFGE analyses suggest that these isolates are genetically similar.

scholarly journals Misidentification ofCandida guilliermondiiasC. famataamong Strains Isolated from Blood Cultures by the VITEK 2 System

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Si Hyun Kim ◽  
Jeong Hwan Shin ◽  
Jeong Ha Mok ◽  
Shine Young Kim ◽  
Sae Am Song ◽  
...  
Keyword(s):  
Gene Sequencing ◽  
Candida Guilliermondii ◽  
Blood Cultures ◽  
Rrna Gene ◽  
28S Rrna ◽  
Sequencing Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Vitek 2 ◽  
Tof Ms

Introduction. The aim of this study was to differentiate betweenCandida famataandCandida guilliermondiicorrectly by using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and gene sequencing.Methods. Twenty-eightCandidastrains from blood cultures that had been identified asC. famata(N=25),C. famata/C. guilliermondii(N=2), andC. guilliermondii(N=1) by the VITEK 2 system using the YST ID card were included. We identified these strains by MALDI-TOF MS and gene sequencing using the 28S rRNA andITSgenes and compared the results with those obtained by the VITEK 2 system.Results. All 28 isolates were finally identified asC. guilliermondii.Sequencing analysis of the 28S rRNA gene showed 99.80%–100% similarity withC. guilliermondiifor all 28 strains. TheITSgene sequencing of the strains showed 98.34%–100% homology withC. guilliermondii.By MALDI-TOF, we could correctly identify 21 (75%) of 28C. guilliermondiiisolates.Conclusion. We should suspect misidentification whenC. famatais reported by the VITEK 2 system, and we always should keep in mind the possibility of misidentification of any organism when an uncommon species is reported.

scholarly journals Identification of Candida parapsilosis Species Complex and Lodderomyces elongisporus Isolates with MALDI-TOF MS

2021 ◽  
Author(s):  
Engin Kaplan ◽  
Ayşe Sultan Karakoyun ◽  
Deniz Alkaya ◽  
Nevzat Ünal ◽  
Aylin Döğen ◽  
...  
Keyword(s):  
Species Complex ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Maldi Tof Ms ◽  
Biochemical Methods ◽  
Maldi Tof ◽  
Candida Orthopsilosis ◽  
Susceptibility Profiles ◽  
Reference Strains ◽  
Tof Ms

Objective: Candida parapsilosis species complex and Lodderomyces elongisporus may have differences in terms of their virulence, prevalence, and antifungal susceptibility profiles. These species are difficult to identify with biochemical methods. Therefore, there is a need for more efficient identification methods in terms of time, cost, and applicability. This study aims to evaluate the diagnostic performance of the MALDI-TOF MS method in discriminating between isolates belonging to the C. parapsilosis species complex and L. elongisporus. Method: In the current study, a total of 32 reference strains, including the C. parapsilosis (n=8), Candida orthopsilosis (n=7), Candida metapsilosis (n=6), and L. elongisporus (n=11) species were identified using the MALDI-TOF MS method. Results: The species names of 31 (93.7%) isolates belonging to the C. parapsilosis species complex and L.elongisporus were correctly identified. Twenty four isolates including eight (100%) C. parapsilosis, five (83%) C. metapsilosis, five (71%) C. orthopsilosis, and six (54%) L. elongisporus isolates were identified with score values ranging from 1.7 to 2.14. According to the secure identification reference score of ≥ 1.7, the sensitivity and specificity of the MALDI-TOF MS method were determined as 54.5–100% and 96.3–100%, respectively. Conclusion: Although the MALDI-TOF MS method has been shown to be effective in the rapid molecular phenotypic diagnosis of species that were difficult to discriminate using biochemical methods such as C. parapsilosis species complex and L. elongisporus, there is a clear need to optimize the method and develop a larger MS library for species-level identification within secure score ranges.

Comparison of MALDI-TOF MS and VITEK 2 system for laboratory diagnosis of Granulicatella and Abiotrophia species causing invasive infections

2013 ◽  
Vol 77 (3) ◽  
pp. 216-219 ◽  
Author(s):  
Paul Ratcliffe ◽  
Hong Fang ◽  
Ellinor Thidholm ◽  
Stina Boräng ◽  
Katarina Westling ◽  
...  
Keyword(s):  
Laboratory Diagnosis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Invasive Infections ◽  
Vitek 2 ◽  
Tof Ms

scholarly journals Performance of MALDI–TOF Mass Spectrometry in the Philippines

2021 ◽  
Vol 6 (3) ◽  
pp. 112
Author(s):  
Morichika Osa ◽  
Maria Cecilia Belo ◽  
Zita Dela Merced ◽  
Annavi Marie G. Villanueva ◽  
Jaira Mauhay ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
The Philippines ◽  
Species Level ◽  
Middle Income ◽  
Maldi Tof Ms ◽  
Middle Income Countries ◽  
Biochemical Methods ◽  
Maldi Tof ◽  
Fungal Isolates ◽  
Tof Ms

Identification of the causative pathogen in infectious diseases is important for surveillance and to guide treatment. In low- and middle-income countries (LMIC), conventional culture and identification methods, including biochemical methods, are reference-standard. Biochemical methods can lack sensitivity and specificity and have slow turnaround times, causing delays in definitive therapy. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI–TOF MS) is a rapid and accurate diagnostic method. Most studies comparing MALDI–TOF MS and biochemical methods are from high-income countries, with few reports from LMIC with tropical climates. The aim of this study was to assess the performance of MALDI–TOF MS compared to conventional methods in the Philippines. Clinical bacterial or fungal isolates were identified by both MALDI–TOF MS and automated (VITEK2) or manual biochemical methods in the San Lazaro Hospital, Metro Manila, the Philippines. The concordance between MALDI­–TOF MS and automated (VITEK2) or manual biochemical methods was analyzed at the species and genus levels. In total, 3530 bacterial or fungal isolates were analyzed. The concordance rate between MALDI–TOF MS and biochemical methods was 96.2% at the species level and 99.9% at the genus level. Twenty-three isolates could not be identified by MALDI–TOF MS. In this setting, MALDI–TOF MS was accurate compared with biochemical methods, at both the genus and the species level. Additionally, MALDI–TOF MS improved the turnaround time for results. These advantages could lead to improved infection management and infection control in low- and middle-income countries, even though the initial cost is high.

Application of Routine Diagnostic Procedure, VITEK 2 Compact, MALDI-TOF MS, and PCR Assays in Identification Procedure of Bacterial Strain with Ambiguous Phenotype

2016 ◽  
Vol 72 (5) ◽  
pp. 570-582 ◽  
Author(s):  
Marta Książczyk ◽  
Maciej Kuczkowski ◽  
Bartłomiej Dudek ◽  
Kamila Korzekwa ◽  
Anna Tobiasz ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Diagnostic Procedure ◽  
Identification Procedure ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Vitek 2 ◽  
Pcr Assays ◽  
Tof Ms

scholarly journals First Food-Borne Cases of Vibrio Parahaemolyticus in Turkey

ANKEM Dergisi ◽  
2021 ◽  
Author(s):  
Nilgün Kansak ◽  
Rıza Adaleti ◽  
Belkıs Levent ◽  
Sebahat Aksaray
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Reference Laboratory ◽  
Seafood Consumption ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Food Borne ◽  
Stool Samples ◽  
Vitek 2 ◽  
Conventional Methods ◽  
Tof Ms

ABSTRACT Vibrio parahaemolyticus (V.parahaemolyticus) is detected in many parts of the world. It is one of the most common causes of food-borne infections in Asian countries and Japan, and is usually seen as minor outbreaks involving less than ten cases. In this study, it is aimed to investigate V.parahaemolyticus in diarrhea cases in our laboratory in order to draw attention to the possible cases of this agent due to the increase in the consumption of shellfish. In the period of July-August 2018; patients who applied to the emergency service of our hospital with gastrointestinal tract infection symptoms following seafood consumption were investigated by stool microscopy and culture as part of routine procedures. Stool samples were cultured on Hektoen enteric agar, MacConkey agar, and sheep blood agar and were incubated at 37°C for 24 hours. After the incubation period, lactose negative and oxidase-positive colonies were identified by classical biochemical tests, VITEK 2 and MALDI-TOF MS (bioMérieux, France). In seven patients aged between 12-59, clinical symptoms associated with gastroenteritidis started after consuming stuffed mussels in four, eating fish in one, and in a patient after consuming fast food. One patient could not be contacted. In the microscopic examination of the macroscopically watery and mucous stool samples, abundant leukocytes in all samples, and abundant erythrocytes in addition to leukocytes in one sample were seen. The bacteria grown in culture were identified as V.parahaemolyticus by conventional methods and automated systems, Vitek 2 with 96 % and MALDITOF MS with 99 % accuracy. The results were also confirmed by the General Directorate of Public Health, National Enteric Pathogens Reference Laboratory by conventional methods, API 20 E and MALDI-TOF MS (BrukerDaltonics, USA). It should be kept in mind that V.parahaemolyticus can be isolated as a cause of gastroenteritis in diarrhea cases during the summer months, especially in the presence of a history of seafood consumption, and further investigations should be performed in this direction.

Sign in / Sign up

Export Citation Format

Share Document