The incidence of wildlife mortalities on the road is widespread phenomenon reported numbers of countries in the world, despites this not much attention gain in our country. To address this issue, investigation on wildlife mortalities species, crushed due to heavy vehicle traffic movements were started to road passing through near Dumna Nature Reserve (DNR), Jabalpur, Madhya Pradesh, Central India during 2011-2013. The 58 species of wild animals were recorded road kill mortality during the investigation. Among these 8 (13.79%) species of amphibians, 14 (24.13%) species of reptiles, 16 (27.58%) species of birds and 20 (34.48%) species of mammals were found dead on the road by speedy vehicle near (DNR). Several wild animals come under Wildlife Protection Act. (1972) with different scheduled and IUCN status also included. The 14 (24.13%) species LR-lc, 24 (41.37%) species LC and 18 (31.03%) species LR-nt, come under different IUCN status. It is needed to save our precious wildlife biodiversity from road kill mortality by taking all possible protection measures to heavy traffic movement.