The Effect of Calcium Level on Microbial Phytase Activity and Nutrient Balance in Swine

Abstract A simple and rapid method is described for determining the enzymatic activity of microbial phytase. The method is based on the determination of inorganic orthophosphate released on hydrolysis of sodium phytate at pH 5.5.

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Effects of dietary Ca/P ratio, P level and microbial phytase supplementation on nutrient digestibilities in growing pigs: breakdown of phytic acid, partition of P and phytase activity along the intestinal tract

Journal of Animal Physiology and Animal Nutrition ◽

10.1046/j.1439-0396.2000.00262.x ◽

2000 ◽

Vol 83 (4-5) ◽

pp. 193-204 ◽

Cited By ~ 9

Author(s):

M. Seynaeve ◽

G. Janssens ◽

M. Hesta ◽

C. Van Nevel ◽

R. O. De Wilde

Keyword(s):

Phytic Acid ◽

Intestinal Tract ◽

Growing Pigs ◽

Phytase Activity ◽

Microbial Phytase

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Soil lead alters phytase activity and mineral nutrient balance of Pisum sativum

Environmental and Experimental Botany ◽

10.1016/s0098-8472(02)00011-4 ◽

2002 ◽

Vol 48 (1) ◽

pp. 61-73 ◽

Cited By ~ 51

Author(s):

Aira E.A Päivöke

Keyword(s):

Pisum Sativum ◽

Nutrient Balance ◽

Phytase Activity ◽

Mineral Nutrient ◽

Soil Lead

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Phosphorus digestibility and phytate degradation in pigs fed wheat-based diets with different intrinsic phytase activity and added microbial phytase

Archives of Animal Nutrition ◽

10.1080/1745039x.2021.1988814 ◽

2021 ◽

pp. 1-15

Author(s):

Nicolas Klein ◽

Marius Papp ◽

Pia Rosenfelder-Kuon ◽

Annika Schroedter ◽

Ulrike Avenhaus ◽

...

Keyword(s):

Phytase Activity ◽

Phytate Degradation ◽

Microbial Phytase

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Effects of supplemental microbial phytase on performance of broiler chickens

Agricultural and Food Science ◽

10.23986/afsci.72717 ◽

1994 ◽

Vol 3 (5) ◽

pp. 457-466

Author(s):

Tuomo Koskinen ◽

Jari Piironen ◽

Tiina Hakonen

Keyword(s):

Broiler Chickens ◽

Bone Mineralization ◽

Control Diet ◽

Live Weight ◽

Positive Control ◽

Negative Control ◽

Phytase Activity ◽

Broiler Chicks ◽

Microbial Phytase ◽

Soya Bean Meal

Three trials were conducted on a total of 5100 broiler chicks (0-5.5 weeks) to study the effects of different microbial phytase (Aspergillus niger) supplementations (250-1000 phytase units = PU/g) on the performance and bone mineralization of birds and on the utilization of phosphorus compared with the effects of mineral P additions as dicalcium phosphate. The basal diets (negative controls) were principally composed of soya bean meal (SBM) and grain (wheat, barley, oats) supplemented with up to 0.10% mineral P; the positive control diets were supplemented with 0.24-0.30% mineral P. Compared with the negative control groups, which were fed diets with 0.05% mineral P either during the whole rearing period or during the starting period only, phytase addition increased live weight by 4-7% and feed intake by 3-9% (Trials 1 and 2). Tibia values indicated that with low mineral P supplementations (0.05 and 0.10%), phytase additions have a non-significant effect on bone mineralization. If mineral P is not added, the tibia values show a marked response to phytase (Trial 3). Utilization of P increased from 50.9% to 60.0% when phytase (1000 PU/g) was added to the diets without mineral P supplementation (Trial 3). Utilization of P was only 34.5% in the positive control diet (0.3% mineral P). The results were obviously affected by the intrinsic phytase activity in grain and the dietary calcium level, and suggest that, in SBM-grain-based diets with very low (0.05%) or no mineral P supplementation, weight gain can be increased by adding phytase up to 1000 PU/g. This supplemented phytase activity is apparently sufficient for adequate bone mineralization.

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Calcium level affects the efficacy of supplemental microbial phytase in corn-soybean meal diets of weanling pigs1

Journal of Animal Science ◽

10.2527/1994.721139x ◽

1994 ◽

Vol 72 (1) ◽

pp. 139-143 ◽

Cited By ~ 88

Author(s):

X. G. Lei ◽

P. K. Ku ◽

E. R. Miller ◽

M. T. Yokoyama ◽

D. E. Ullrey

Keyword(s):

Soybean Meal ◽

Calcium Level ◽

Microbial Phytase

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Sites of phytase and xylanase activities in the gastrointestinal tract of broiler chickens

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200019311 ◽

2007 ◽

Vol 2007 ◽

pp. 28-28

Author(s):

A.A. Sadeghi ◽

P. Shawrang ◽

K. Karimi

Keyword(s):

Small Intestine ◽

Gastrointestinal Tract ◽

Inorganic Phosphate ◽

Broiler Chickens ◽

Xylanase Activity ◽

Phytase Activity ◽

Major Site ◽

Activity Measurements ◽

Exogenous Enzymes ◽

Microbial Phytase

Because digesta vary in pH in different gastrointestinal segments of poultry, exogenous phytase or xylanase may exhibit differences in activity along the gastrointestinal tract. Previous reports indicated that the stomach is the major site of exogenous microbial phytase activity, with no further activity found in the small intestine of piglets. Information regarding exogenous phytase or xylanase activity in the gastrointestinal tract of poultry is largely unavailable. Because exogenous phytase or xylanase activity in the digesta is extremely low, normal phytase or xylanase activity measurements are prone to errors resulting from background interference contributed by the exogenous inorganic phosphate or xylose in the digesta (Walsh et al., 1995). The aim of this study was to utilize electrophoresis activity stain to detect the activity of phytase or xylanase in different gastrointestinal segments of broiler chickens fed diets containing exogenous enzymes.

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Hydrolysis of phytic acid by intrinsic plant and supplemented microbial phytase (Aspergillus niger) in the stomach and small intestine of minipigs fitted with re-entrant cannulas 2. Phytase activity

Journal of Animal Physiology and Animal Nutrition ◽

10.1046/j.1439-0396.2001.00350.x ◽

2001 ◽

Vol 85 (11-12) ◽

pp. 414-419 ◽

Cited By ~ 15

Author(s):

C. Rapp ◽

H.-J. Lantzsch ◽

W. Drochner

Keyword(s):

Small Intestine ◽

Aspergillus Niger ◽

Phytic Acid ◽

Phytase Activity ◽

Microbial Phytase ◽

Hydrolysis Of

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Ileal digestibility of calcium and phosphorus in broilers fed diets with different phytases and Ca:available P ratios

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2018001100004 ◽

2018 ◽

Vol 53 (11) ◽

pp. 1222-1229 ◽

Cited By ~ 1

Author(s):

David Henrique de Oliveira ◽

Luciana de Paula Naves ◽

Nicole Batelli de Souza Nardelli ◽

Márcio Gilberto Zangerônimo ◽

Paulo Borges Rodrigues

Keyword(s):

Phytase Activity ◽

The Other ◽

Ileal Digestibility ◽

Microbial Phytase ◽

Calcium And Phosphorus ◽

Different Sources ◽

Ileal Content

Abstract: The objective of this work was to determine the apparent ileal digestibility of calcium and phosphorus in broilers fed diets with different phytases and calcium:available phosphorus (Ca:aP) ratios. Two experiments were carried out: one with broilers with 22 to 33 days of age, and the other with broilers with 35 to 42 days. The Ca:aP ratios used were 4.5:1.0, 6.0:1.0, and 7.5:1.0 in the first period, and 3.5:1.0, 5.0:1.0, and 6.5:1.0 in the second. All diets were supplemented with 1,500 units of phytase activity per kilogram of six different sources of microbial phytase. At the end of each experiment, two broilers per replicate were slaughtered to collect the ileal content. In the samples of digested food, the Ca and P contents were determined for later calculation of the apparent ileal digestibility coefficients of these minerals. The Ca:aP ratio influenced the activity of phytases, and the highest digestibility was observed with lower ratios, in both experiments. The phytases that provided greater digestibility coefficients were A, D, and E. In both ages evaluated, the inclusion of phytases in the feed improved the digestibility of Ca and P, which was also affected by the content of calcium and by the phytase source used.

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