scholarly journals Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes

2004 ◽  
Vol 17 (10) ◽  
pp. 1369-1373 ◽  
Author(s):  
C. S. Park ◽  
M. Y. Kim ◽  
Y. J. Yi ◽  
Y. J. Chang ◽  
S. H. Lee ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Quality ◽  
Vitro Fertilization ◽  
Boar Sperm

Effects of Isatis root polysaccharide on boar sperm quality during liquid storage and in vitro fertilization

2019 ◽  
Vol 210 ◽  
pp. 106178 ◽  
Author(s):  
Zhiqiang Ren ◽  
Weike Shaoyong ◽  
Qian Li ◽  
Lu Ma ◽  
Junying Xiao ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Quality ◽  
Vitro Fertilization ◽  
Liquid Storage ◽  
Boar Sperm

275 PENTOXIFYLLINE ADDED TO FREEZING EXTENDER HAS A DELETERIOUS EFFECT ON POST-THAW BOAR SPERM QUALITY AND IN VITRO FERTILIZATION RATE

2006 ◽  
Vol 18 (2) ◽  
pp. 245
Author(s):  
M. A. Gil ◽  
J. Roca ◽  
M. Hernandez ◽  
C. Cuello ◽  
C. Almiñana ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Deleterious Effect ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Control Group ◽  
Vitro Fertilization ◽  
Split Plot ◽  
Boar Sperm ◽  
Plot Design

Pentoxifylline, a methylxanthine derivative, is considered to be a hyperactivation and acrosome reaction-improving agent. The purpose of this study was to test how the addition of pentoxifylline to freezing extender influences post-thaw sperm motility and membrane integrity. The ability of thawed spermatozoa to fertilize in vitro-matured oocytes was also assessed. Pooled ejaculate sperm-rich fractions collected from three fertile boars were frozen in 0.5-mL straws after being diluted in lactose/egg yolk/glycerol/Orvus-ES-Paste extender (0 pentoxifylline = control) or the same extender supplemented with 2, 4, 8, 16, and 32 mM pentoxifylline. To evaluate post-thaw sperm survival, the percentage of total motile spermatozoa and rapid progressive spermatozoa (CASA system) and plasma membrane and acrosome integrity (flow cytometry) were assessed. Data from six replicates were analyzed in a split plot design using a PROMIXED model. The addition of 4, 8, 16, and 32 mM pentoxifylline to freezing extender significantly decreased progressive and total motility (P < 0.001) compared with control (4.5/26.6%, 4.5/24.5%, 2.8/20.5%, 0.6/11.4%, and 13.2/49.7% for the 4, 8, 16, and 32 mM pentoxifylline groups and the control group, respectively). The same was observed for sperm viability; the percentage of viable spermatozoa with intact acrosomes was significantly lower (P < 0.001) in pentoxifylline-treated groups compared with the control group, chiefly in the 16 mm, and 32 mM pentoxifylline groups (54, 11.6, and 6.2% for control, 16 and 32 mM, respectively). To evaluate in vitro fertilization parameters, cumulus-oocyte complexes were matured in BSA-free NCSU23 medium with 10% porcine follicular fluid, 0.1% cysteine, 10 ng EGF, 10 IU/mL eCG, and 10 IU/mL hCG, in a incubator at 39�C and 5% CO2. After 40-44 h of maturation, oocytes (n = 1067, in three replicates) were denuded of cumulus cells, washed, transferred to droplets (30 oocytes in 50 �L) of TBM medium supplemented with 2 mM caffeine and 0.2% BSA, and inseminated (2000 thawed sperm/oocyte). After a co-incubation period of 6 h, oocytes were washed and transferred to droplets (500 �L) of NCSU23 with 0.4% BSA for another 10-14 h, then fixed and stained for 72 h, and examined under a phase-contrast microscope. Data were analyzed as split plot design using a PROMIXED model. The addition of pentoxifylline to the freezing extender reduced significantly (P < 0.001) the penetration rate (51.4, 17.5, 15.8, 17.8, 9.5, and 4.8% for the 0, 2, 4, 8, 16, and 32 mM pentoxifylline groups, respectively) and the efficiency (monospermic oocytes/total inseminated oocytes) of fertilization (34.8, 14.6, 14.7, 15.5, 7.9, and 4.8% for the 0, 2, 4, 8, 16, and 32 mM pentoxifylline groups, respectively) as compared with the control group (the first value in each of these two cases). It is therefore concluded that pentoxifylline added to the freezing extender has a deleterious effect on post-thaw boar sperm quality and on their ability to fertilize the oocytes in vitro. This work was supported by CICYT (AGL05-0471).

Sperm quality and in vitro fertilization

1984 ◽  
pp. 125-128
Author(s):  
V. Baukloh ◽  
H.-H. Riedel ◽  
S. Paul ◽  
L. Mettler
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Quality ◽  
Vitro Fertilization

Effect of Astragalus polysaccharide addition to thawed boar sperm on in vitro fertilization and embryo development

2018 ◽  
Vol 121 ◽  
pp. 21-26 ◽  
Author(s):  
Xiao-gang Weng ◽  
Ming-ming Cai ◽  
Yu-ting Zhang ◽  
Yan Liu ◽  
Zheng-ling Gao ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Vitro Fertilization ◽  
Astragalus Polysaccharide ◽  
Boar Sperm

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

2013 ◽  
Vol 16 (4) ◽  
pp. 773-785 ◽  
Author(s):  
K. Lasiene ◽  
V. Gedrimas ◽  
A. Vitkus ◽  
S. Glinskyte ◽  
V. Lasys ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
Sperm Quality ◽  
Human Sperm ◽  
Developmental Competence ◽  
Vitro Fertilization ◽  
Abnormal Sperm ◽  
Morphological Criteria ◽  
Negative Effect

Abstract The quality of sperm has a direct influence on the fertilization and developmental competence of embryos. In the literature we did not find defined criteria for evaluation of normal sperm parameters in various species of domestic mammals. Therefore we attempted to review evaluation of criteria of morphologically normal human sperm and their abnormalities. All sperm cells observed in the stained sample are classified as normal or abnormal. Any abnormalities in morphology of sperm have a negative effect on the outcome in in vitro fertilization and intracytoplasmic sperm injection. Abnormal sperm are categorized into subgroups according to the observed defects (concerning the head and/or midpiece and/or tail). Most morphologically abnormal sperm have multiple defects. This article can be considered as guideline for the manual of sperm quality evaluation in different species of domestic mammals.

Factors affecting homologous in vitro fertilization assay of boar sperm fertility

1994 ◽  
Vol 41 (1) ◽  
pp. 249
Author(s):  
C. Matás ◽  
E. Martínez ◽  
J.M. Vázquez ◽  
J. Roca ◽  
J. Gadea ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Factors Affecting ◽  
Boar Sperm

scholarly journals 289EFFECT OF FERTILIZATION TIME OF PIG OOCYTES MATURED IN-VITRO BY BOAR SPERM STORED AT 4°C

2004 ◽  
Vol 16 (2) ◽  
pp. 264
Author(s):  
Y.J. Yi ◽  
M.Y. Kim ◽  
Y.J. Chang ◽  
D.I. Jin ◽  
C.S. Park
Keyword(s):  
In Vitro Fertilization ◽  
Room Temperature ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Penicillin G ◽  
In Vitro Production ◽  
Vitro Fertilization ◽  
Boar Sperm ◽  
Sperm Penetration

The use of boar sperm stored at 4°C may be a useful tool for in vitro production of pig embryos. Therefore, this study was undertaken to investigate the effect of fertilization time of pig oocytes matured in-vitro by boar sperm. The sperm-rich fraction (30–60mL) was slowly cooled to room temperature (20–23°C) by 2h after collection. Semen was transferred into 15-mL tubes, centrifuged at room temperature for 10min at 800g, and the supernatant solution was poured off. The concentrated sperm was resuspended with 5mL of the LEN (11.0g lactose hydrate, 20mL egg yolk, 0.05g N-acetyl-D-glucosamine and 100mL distilled water) diluent to provide 1.0×109 spermmL−1 at room temperature. The resuspended semen was cooled in a refrigerator to 4°C. The medium used for oocyte maturation was TCM-199 supplemented with 26.19mM sodium bicarbonate, 0.9mM sodium pyruvate, 10μgmL−1 insulin, 2μgmL−1 vitamin B12, 25mM HEPES, 10μgmL−1 bovine apotransferrin, 150μM cysteamine, 10IUmL−1 PMSG, 10IUmL−1 hCG, 10ngmL−1 EGF, 0.4% BSA, 75μgmL−1 sodium penicillin G, 50μgmL−1 streptomycin sulfate and 10% pFF. After about 22h of maturation, oocytes were cultured without cysteamine and hormones for 22h at 38.5°C, 5% CO2 in air. Oocytes were inseminated with boar sperm stored at 4°C for 2 days after collection. Oocytes were coincubated for 1, 3, 6 and 9h in 500μL TBM fertilization media with 1×106mL−1 sperm concentration. Thereafter, oocytes were transferred into 500μL NCSU-23 culture medium containing 0.4% BSA for further culture of 6, 48 and 144h, fixed and stained for the evaluation of fertilization parameters and developmental ability. Data were analysed by ANOVA and Duncan’s multiple range test using the SAS program. The rates of sperm penetration and male pronuclear formation were higher in the fertilization times of 6 and 9h than in those of 1 and 3h. The percentage of polyspermic oocytes was highest in fertilization time of 9h compared with other incubation times. The rates of cleaved oocytes were higher in the fertilization times of 6 and 9h (85.0 and 84.6%) compared with those of 1 and 3h (61.1 and 76.8%). The percentage of blastocyst formation from the cleaved oocytes was highest in the fertilization time of 6h (33.6%) than in that of 1, 3 and 9h (11.4, 23.0 and 29.6%). Mean cell numbers per blastocyst were 32.9±3.3, 27.6±2.7, 26.3±2.2 and 24.4±1.8 in the fertilization times of 6, 9, 3 and 1h, respectively. In conclusion, we found out that boar sperm stored at 4°C could be used for in vitro fertilization of pig oocytes matured in vitro. Also, we recommend the coincubation time of 6h in 500μL TBM fertilization medium with 1×106mL−1 sperm concentration for in vitro fertilization of pig oocytes matured in vitro.

Sign in / Sign up

Export Citation Format

Share Document