Gluten in Rice Flour and Baked Rice Products by G12 Sandwich ELISA: First Action 2014.03

2015 ◽  
Vol 98 (1) ◽  
pp. 103-111 ◽  
Author(s):  
Elisabeth Halbmayr-Jech ◽  
Adrian Rogers ◽  
Clyde Don ◽  
Michael Prinster ◽  
G Augustin ◽  
...  
Keyword(s):  
Sandwich Elisa ◽  
Collaborative Study ◽  
Rice Flour ◽  
Elisa Test ◽  
Technical Committee ◽  
Free Products ◽  
Heat Treated ◽  
Test Kit ◽  
Low Levels ◽  
Ppm Level

Abstract The Protein and Enzymes Technical Committee of American Association of Cereal Chemists initiated a collaborative study to confirm whether the G12 antibody-based sandwich ELISA test kit is able to detect gluten in the lower mg/kg (ppm) level. Twenty laboratories investigated 24 heat-treated and non-heat-treated blind-coded samples with incurred gluten levels up to 100 mg/kg. The method has been validated for testing foods to conform to the defined Codex thresholds for gluten in gluten-free products at less than 20 mg gluten/kg. The collaborative study showed that low levels of gluten could be detected by G12 Sandwich ELISA with reproducibility RSDR of 32%and repeatability RSDr of 16%. Incurred samples showed a recovery between 62 and 135%. It is recommended that the method be accepted by AOAC as Official First Action.

Gliadin as a Measure of Gluten in Foods Containing Wheat, Rye, and Barley—Enzyme Immunoassay Method Based on a Specific Monoclonal Antibody to the Potentially Celiac Toxic Amino Acid Prolamin Sequences: Collaborative Study

2012 ◽  
Vol 95 (4) ◽  
pp. 1118-1124 ◽  
Author(s):  
Ulrike Immer ◽  
Sigrid Haas-Lauterbach ◽  
V Cerne ◽  
F Chirdo ◽  
J de Sadeleer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Amino Acid ◽  
Working Group ◽  
Collaborative Study ◽  
Poor Performance ◽  
Elisa Test ◽  
Specific Monoclonal Antibody ◽  
Negative Sample ◽  
Heat Treated ◽  
Ppm Level

Abstract The Working Group on Prolamin Analysis and Toxicity (WGPAT) organized a collaborative study to confirm whether the two R5 antibody-based ELISA test kits are able to detect gliadin in the lower mg/kg (ppm) level. Twenty laboratories investigated 12 blind-coded samples, spiked and naturally contaminated, to show the possibility of determining traces of gliadin in heat-treated or nonheat-treated foods by ELISA. It was shown that very small amounts of gliadin (below 100 ppm) could be detected by ELISA with a reproducibility RSDR (37%) and a repeatability RSDr (27%) common for ELISA under these conditions. The recovery of gliadin from the spiked samples was between 84 and 109%, based on the results of all laboratories, including those with poor performance. No false positives were found by the method (P ≤ 0.05), but one negative sample was contaminated during the bakery process. It is recommended that the method be accepted by AOAC as Official First Action.

scholarly journals Rapid Enzyme-Linked Immunoassay for Detection of Salmonella in Food and Feed Products: Performance Testing Program

2000 ◽  
Vol 83 (2) ◽  
pp. 299-304 ◽  
Author(s):  
Frederick J Bolton ◽  
Erica Fritz ◽  
Sharon Poynton ◽  
Torben Jensen
Keyword(s):  
Performance Testing ◽  
Elisa Test ◽  
Food Matrix ◽  
Salmonella Spp ◽  
Testing Program ◽  
Test Kit ◽  
Food And Feed ◽  
Broad Variety ◽  
Low Levels

Abstract The BIOLINE Salmonella ELISA Test for Salmonella spp., which is a rapid, easy, and convenient assay was evaluated for use in detecting Salmonella in foods and feeds. Each food matrix or feed was artificially contaminated with low levels of Salmonella. Twenty different matrixes were studied and 20 different Salmonella strains from a broad variety of serogroups (B, C, D, E, F, G, H, I, M, O, P, and U) were used. The ELISA Test kit detected levels as low as 1 cfu/25 g sample with at least 4 of the 20 matrixes tested. The test kit is applicable to all sample types tested. The BIOLINE Salmonella ELISA Test kit has been granted AOAC–RI performance tested status.

Partially Hydrolyzed Gluten in Fermented Cereal-Based Products by R5 Competitive ELISA: Collaborative Study, First Action 2015.05

2015 ◽  
Vol 98 (5) ◽  
pp. 1346-1354 ◽  
Author(s):  
Markus Lacorn ◽  
Thomas Weiss
Keyword(s):  
Collaborative Study ◽  
Technical Committee ◽  
Competitive Elisa ◽  
New Method ◽  
Fermented Foods ◽  
Gluten Free ◽  
Free Products ◽  
Elisa System ◽  
Fermented Products

Abstract In 2008, the AACC International Protein Technical Committee (now Protein and Enzymes Technical Committee) initiated a collaborative study of a method for determining gluten in fermented products, using an R5 competitive ELISA system. The method has been approved as AACCI Approved Method AACCI 38-55.02. The new method has been validated for testing fermented foods and beverages to determine that they conform to the Codex threshold of 20 mg of gluten/kg in total for gluten-free products. It is recommended that the method be accepted by AOAC as Official First Action.

Determination of Gluten in Processed and Nonprocessed Corn Products by Qualitative R5 Immunochromatographic Dipstick: Collaborative Study, First Action 2015.16

2016 ◽  
Vol 99 (3) ◽  
pp. 730-737 ◽  
Author(s):  
Markus Lacorn ◽  
Katharina Scherf ◽  
Steffen Uhlig ◽  
Thomas Weiss ◽  
G Augustin ◽  
...  
Keyword(s):  
Qualitative Analysis ◽  
Collaborative Study ◽  
Technical Committee ◽  
Gluten Free ◽  
Free Products ◽  
Collaborative Test ◽  
Corn Products

Abstract In September 2013, the AACC International (AACI) Protein Technical Committee (now Protein and Enzymes Technical Committee) initiated a collaborative study of a method for the qualitative analysis of intact gluten in processed and nonprocessed corn products, using an R5 immunochromatographic dipstick system. It was validated to demonstrate that potential gluten-free products contain gluten lower than the Codex threshold of 20 mg/kg gluten. The results of the collaborative test with 18 participants confirmed that the method is suitable to detect gluten contaminations that are clearly lower than the threshold. It is recommended that the method be accepted by AOAC as Official First Action.

AACCI Approved Methods Technical Committee Report: Collaborative Study on the Immunochemical Determination of Intact Gluten Using an R5 Sandwich ELISA

2013 ◽  
Vol 58 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Peter Koehler ◽  
Theresa Schwalb ◽  
Ulrike Immer ◽  
Markus Lacorn ◽  
Paul Wehling ◽  
...  
Keyword(s):  
Sandwich Elisa ◽  
Collaborative Study ◽  
Technical Committee ◽  
Committee Report ◽  
Immunochemical Determination

AACCI Approved Methods Technical Committee Report: Collaborative Study on a Method for Determining Firmness of Cooked Pulses (AACCI Method 56-36.01)

2012 ◽  
Vol 57 (5) ◽  
pp. 230-234 ◽  
Author(s):  
N. Wang ◽  
J. F. Panozzo ◽  
J. Wood ◽  
L. J. Malcolmson ◽  
G. C. Arganosa ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Technical Committee ◽  
Committee Report

scholarly journals HIV and Hepatitis B seroprevalence among the nepalese blood donors

2013 ◽  
Vol 3 (5) ◽  
pp. 390-393 ◽  
Author(s):  
SR Kandel ◽  
P Ghimire ◽  
BR Tiwari ◽  
M Rajkarnikar
Keyword(s):  
Hepatitis B ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Elisa Test ◽  
Age Group ◽  
Male And Female ◽  
Test Kit ◽  
The Difference ◽  
Donated Blood ◽  
Donor Recruitment

Background: HIV and Hepatitis B infections are public health problems in Nepal. This study was conducted based at NRCS/CBTS, with the objective of determining the HIV and HBsAg sero-prevalence in non-remunerated volunteer blood donors. Materials and Methods: A total of 66,904 units of blood collected, following donor recruitment criteriaduring March 2009-Sept. 2010 was included for analysis. All donated blood samples were subjected to screening for Transfusion transmitted infections including HIV and Hepatitis B surface antigen using standard ELISA test kits (Dade Behring, Germany). Initial reactive sera were re-tested for reconfi rmation with same test kits plus another test kit (Detect-HIV, Adaltis Inc, and Qualisa). Results: Out of 66,904 units of blood collected, 56,973 units were from male and 9,931 were from female donors. Among the total screened samples, 73 (0.10%) were found to be positive for HIV, {0.11% (64/56973) in male and 0.09% (9/9931) in female}; the difference between male and female donors (?2<3.841) was statistically signifi cant. The seroprevalence of HIV was highest in age group of 30- 39 both in male and female (p<0.001). Similarly, for HBsAg, overall seroprevalence was found to be 0.47% (316/66904 {0.42% (242/56973) in male and 0.74% (74/9931) in female}. The difference was statistically signifi cant (?2<3.841). The highest HBsAg sero-prevalence(0.65%) was also observed in same age group i.e. 30-39 (p<0.001) in male but highest seroprevalence (2.63%) was observed inage group of ?50 in female. Conclusion: Both HIV and HBV sero-prevalence is high in adult voluntary blood donors. Journal of Pathology of Nepal (2013) Vol. 3, No.1, Issue 5, 390-393 DOI: http://dx.doi.org/10.3126/jpn.v3i5.7864

scholarly journals Evaluation of Vaccination Strategy Against Rabies in Hong Kong Macaques

2021 ◽  
Author(s):  
Omid Nekouei ◽  
Paolo Martelli ◽  
Sophie St-Hilaire ◽  
Hui Suk Wai ◽  
Karthiyani Krishnasamy ◽  
...  
Keyword(s):  
Hong Kong ◽  
Vaccination Strategy ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Test Kit ◽  
In The Wild ◽  
The Government ◽  
Specific Association ◽  
Antibody Levels

Rabies is a fatal zoonotic disease that can affect all mammals. Following the directives of the rabies ordinance of the Government of Hong Kong, all wild macaques captured under an ongoing sterilization program (since 2000) were vaccinated against rabies. The main objective of this study was to assess the serological response to rabies vaccination in the population of Hong Kong macaques. An inactivated rabies vaccine was subcutaneously administered to captured macaques under anesthesia. In a 2015 field survey, blood samples from the animals were collected and stored in -80℃ freezer. In July 2021, all frozen sera from vaccinated animals were prepared and tested for antibodies against rabies virus using a commercial enzyme-linked immunosorbent assay (ELISA) test. The test results were dichotomized at the recommended cut-off point of the test kit. Sixty-five samples from the vaccinated macaques were available for this study. All of these animals had received at least one dose of vaccine (1 vaccination) between 2008 and 2015. The interval between the 1 vaccination and blood sampling dates ranged from 21 to 2,779 days. Only five of the 65 macaques had a second vaccination record at the time of sampling; all five had high antibody levels. Among the remaining macaques, 77% (46/60) were positive for rabies antibodies. No specific association was observed between the post-vaccination period and the antibody titer of these macaques and no adverse reactions to vaccination were reported. The current vaccination strategy in Hong Kong macaques appears to effectively elicit rabies antibodies in a high proportion of macaque populations in the wild (78-87%). However, reaching the precise level of protection against a potential challenge with the virus should further be investigated.

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