scholarly journals Potential Gene Therapy: Intravenous Administration of Phagocytes Transfected Ex Vivo with FGF4 DNA/Biodegradable Gelatin Complex Promotes Angiogenesis in Animal Model of Myocardial Ischemia/Reperfusion Injury

10.5772/24078 ◽  
2011 ◽  
Author(s):  
Toru Shizuma ◽  
Chiharu Tanaka ◽  
Hidezo Mori ◽  
Naoto Fukuyam
Keyword(s):  
Gene Therapy ◽  
Animal Model ◽  
Myocardial Ischemia ◽  
Reperfusion Injury ◽  
Intravenous Administration ◽  
Ischemia Reperfusion Injury ◽  
Ex Vivo ◽  
Ischemia Reperfusion ◽  
Myocardial Ischemia Reperfusion Injury ◽  
Myocardial Ischemia Reperfusion

scholarly journals Antioxidant network expression abrogates oxidative posttranslational modifications in mice

2011 ◽  
Vol 300 (5) ◽  
pp. H1960-H1970 ◽  
Author(s):  
R. Mital ◽  
W. Zhang ◽  
M. Cai ◽  
Z. M. Huttinger ◽  
L. A. Goodman ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Myocardial Ischemia ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ex Vivo ◽  
Ischemia Reperfusion ◽  
Fatty Acid Binding ◽  
Myocardial Ischemia Reperfusion Injury ◽  
Myocardial Ischemia Reperfusion

Antioxidant enzymatic pathways form a critical network that detoxifies ROS in response to myocardial stress or injury. Genetic alteration of the expression levels of individual enzymes has yielded mixed results with regard to attenuating in vivo myocardial ischemia-reperfusion injury, an extreme oxidative stress. We hypothesized that overexpression of an antioxidant network (AON) composed of SOD1, SOD3, and glutathione peroxidase (GSHPx)-1 would reduce myocardial ischemia-reperfusion injury by limiting ROS-mediated lipid peroxidation and oxidative posttranslational modification (OPTM) of proteins. Both ex vivo and in vivo myocardial ischemia models were used to evaluate the effect of AON expression. After ischemia-reperfusion injury, infarct size was significantly reduced both ex vivo and in vivo, ROS formation, measured by dihydroethidium staining, was markedly decreased, ROS-mediated lipid peroxidation, measured by malondialdehyde production, was significantly limited, and OPTM of total myocardial proteins, including fatty acid-binding protein and sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA)2a, was markedly reduced in AON mice, which overexpress SOD1, SOD3, and GSHPx-1, compared with wild-type mice. These data demonstrate that concomitant SOD1, SOD3, and GSHPX-1 expression confers marked protection against myocardial ischemia-reperfusion injury, reducing ROS, ROS-mediated lipid peroxidation, and OPTM of critical cardiac proteins, including cardiac fatty acid-binding protein and SERCA2a.

Cardioprotective Efficacy of a Novel Antioxidant Mix VitaePro Against Ex Vivo Myocardial Ischemia–Reperfusion Injury

2011 ◽  
Vol 67 (2) ◽  
pp. 281-286 ◽  
Author(s):  
Ram Sudheer Adluri ◽  
Mahesh Thirunavukkarasu ◽  
Lijun Zhan ◽  
Nilanjana Maulik ◽  
Katja Svennevig ◽  
...  
Keyword(s):  
Myocardial Ischemia ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ex Vivo ◽  
Ischemia Reperfusion ◽  
Myocardial Ischemia Reperfusion Injury ◽  
Myocardial Ischemia Reperfusion

scholarly journals Intravenous Administration of Lycopene, a Tomato Extract, Protects against Myocardial Ischemia-Reperfusion Injury

Nutrients ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 138 ◽  
Author(s):  
Chao Tong ◽  
Chuan Peng ◽  
Lianlian Wang ◽  
Li Zhang ◽  
Xiaotao Yang ◽  
...  
Keyword(s):  
Myocardial Ischemia ◽  
Reperfusion Injury ◽  
Intravenous Administration ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Tomato Extract ◽  
Myocardial Ischemia Reperfusion Injury ◽  
Myocardial Ischemia Reperfusion
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