scholarly journals The Use of Amniotic Membrane in the Management of Complex Chronic Wounds

10.5772/64491 ◽  
2016 ◽  
Author(s):  
Gregorio Castellanos ◽  
Ángel Bernabé-García ◽  
Carmen García Insausti ◽  
Antonio Piñero ◽  
José M. Moraleda ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Chronic Wounds

Dehydrated amniotic membrane allografts for the treatment of chronic wounds: a case series

2012 ◽  
Vol 21 (6) ◽  
pp. 290-296 ◽  
Author(s):  
J. Forbes ◽  
D.E. Fetterolf
Keyword(s):  
Amniotic Membrane ◽  
Chronic Wounds ◽  
Case Series

Amniotic membrane grafting in patients with epidermolysis bullosa with chronic wounds

2010 ◽  
Vol 62 (6) ◽  
pp. 1038-1044 ◽  
Author(s):  
Venetia Lo ◽  
Irene Lara-Corrales ◽  
Alejandra Stuparich ◽  
Elena Pope
Keyword(s):  
Amniotic Membrane ◽  
Epidermolysis Bullosa ◽  
Chronic Wounds

scholarly journals Freeze-dried bovine amniotic membrane as a cell delivery scaffold in a porcine model of radiation-induced chronic wounds

2021 ◽  
Vol 48 (4) ◽  
pp. 448-456
Author(s):  
Daemyung Oh ◽  
Daegu Son ◽  
Jinhee Kim ◽  
Sun-Young Kwon
Keyword(s):  
Amniotic Membrane ◽  
Chronic Wounds ◽  
Porcine Model ◽  
Chronic Wound ◽  
Stromal Vascular Fraction ◽  
Cell Delivery ◽  
Analysis Group ◽  
Stem Cell Delivery ◽  
Freeze Dried ◽  
Radiation Induced

Background Locoregional stem cell delivery is very important for increasing the efficiency of cell therapy. Amnisite BA (Amnisite) is a freeze-dried amniotic membrane harvested from bovine placenta. The objective of this study was to investigate the retention of cells of the stromal vascular fraction (SVF) on Amnisite and to determine the effects of cell-loaded Amnisite in a porcine radiation-induced chronic wound model.Methods Initially, experiments were conducted to find the most suitable hydration and incubation conditions for the attachment of SVF cells extracted from pig fat to Amnisite. Before seeding, SVFs were labeled with PKH67. The SVF cell-loaded Amnisite (group S), Amnisite only (group A), and polyurethane foam (group C) were applied to treat radiation-induced chronic wounds in a porcine model. Biopsy was performed at 10, 14, and 21 days post-operation for histological analysis.Results Retaining the SVF on Amnisite required 30 minutes for hydration and 1 hour for incubation. A PKH67 fluorescence study showed that Amnisite successfully delivered the SVF to the wounds. In histological analysis, group S showed increased re-epithelialization and revascularization with decreased inflammation at 10 days post-operation.Conclusions SVFs had acceptable adherence on hydrated Amnisite, with successful cell delivery to a radiation-induced chronic wound model.

scholarly journals Amniotic Membrane Modifies the Genetic Program Induced by TGFß, Stimulating Keratinocyte Proliferation and Migration in Chronic Wounds

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0135324 ◽  
Author(s):  
Antonia Alcaraz ◽  
Anna Mrowiec ◽  
Carmen Luisa Insausti ◽  
Ángel Bernabé-García ◽  
Eva María García-Vizcaíno ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Chronic Wounds ◽  
Genetic Program ◽  
Keratinocyte Proliferation ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Human amniotic membrane: A biological cost effective dressing in chronic wounds.

2020 ◽  
Vol 27 (06) ◽  
pp. 1249-1254
Author(s):  
Ibrahim Yamin ◽  
Ayesha ◽  
Ramla ◽  
Muhammad Ajmal
Keyword(s):  
Wound Healing ◽  
Amniotic Membrane ◽  
Chronic Wounds ◽  
Biological Membrane ◽  
Case Series ◽  
Cost Effective ◽  
Complete Healing ◽  
Lower Limbs ◽  
Human Amniotic Membrane ◽  
Healing Wound

Objectives: The use of human amniotic membrane is essential new concept in wound healing which functions as a biodegradable scaffold on wound surface, as it is a rich hub of stem cells which play an important role in wound healing. Study Design: Randomized Control Trial. Setting: Department of Surgery THQ Hospital Gojra. Period: 1st January 2019 to 30 September 2019. Material & Methods: Experimental study using clinical trial. A case series of 50 patient cases were picked from surgical OPD. Who fall in criteria of chronic non-healing wound with at least three months duration comprising of diabetic, venous ulcers and traumatic non healing wound and neuropathic ulcers. All located on lower limbs. Results: All 50 patient were treated with standard protocol by applying freshly prepared amniotic membrane out of which 4 chronic wounds more than 4 year duration were not healed and 2 cases escaped from the study. HAM dressing was changed after every 7 days and its effect were studied by seeing measuring the reduction in wound size and improvement in pain, swelling and mental stress. Success rate was found about 90% with complete healing. Conclusion: There is a dire need in developing countries to promote the use of HAM, in chronic non healing wounds which is a biological membrane, readily available (free if fresh) with simple sterilization techniques, easy storage and easy application with ultimate goal in achieving speedy cost effective wound healing.

scholarly journals Chronic Wound Healing by Amniotic Membrane: TGF-β and EGF Signaling Modulation in Re-epithelialization

2021 ◽  
Vol 9 ◽  
Author(s):  
Catalina Ruiz-Cañada ◽  
Ángel Bernabé-García ◽  
Sergio Liarte ◽  
Mónica Rodríguez-Valiente ◽  
Francisco José Nicolás
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Amniotic Membrane ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Focal Adhesions ◽  
Fine Tuning ◽  
Minimal Amount ◽  
Complete Suppression ◽  
Egf Signaling

The application of amniotic membrane (AM) on chronic wounds has proven very effective at resetting wound healing, particularly in re-epithelialization. Historically, several aspects of AM effect on wound healing have been evaluated using cell models. In keratinocytes, the presence of AM induces the activation of mitogen-activated protein (MAP) kinase and c-Jun N-terminal kinase (JNK) pathways, together with the high expression of c-Jun, an important transcription factor for the progression of the re-epithelialization tongue. In general, the levels of transforming growth factor (TGF)-β present in a wound are critical for the process of wound healing; they are elevated during the inflammation phase and remain high in some chronic wounds. Interestingly, the presence of AM, through epidermal growth factor (EGF) signaling, produces a fine-tuning of the TGF-β signaling pathway that re-conducts the stalled process of wound healing. However, the complete suppression of TGF-β signaling has proven negative for the AM stimulation of migration, suggesting that a minimal amount of TGF-β signaling is required for proper wound healing. Regarding migration machinery, AM contributes to the dynamics of focal adhesions, producing a high turnover and thus speeding up remodeling. This is clear because proteins, such as Paxillin, are activated upon treatment with AM. On top of this, AM also produces changes in the expression of Paxillin. Although we have made great progress in understanding the effects of AM on chronic wound healing, a long way is still ahead of us to fully comprehend its effects.

scholarly journals Correction: Amniotic Membrane Modifies the Genetic Program Induced by TGFß, Stimulating Keratinocyte Proliferation and Migration in Chronic Wounds

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141026
Author(s):  
Antonia Alcaraz ◽  
Anna Mrowiec ◽  
Carmen Luisa Insausti ◽  
Ángel Bernabé-García ◽  
Eva María García-Vizcaíno ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Chronic Wounds ◽  
Genetic Program ◽  
Keratinocyte Proliferation ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Using of Amniotic Membrane Derivatives for the Treatment of Chronic Wounds

Membranes ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 941
Author(s):  
Iveta Schmiedova ◽  
Alena Dembickaja ◽  
Ludmila Kiselakova ◽  
Beata Nowakova ◽  
Petr Slama
Keyword(s):  
Wound Healing ◽  
Case Studies ◽  
20Th Century ◽  
Amniotic Membrane ◽  
Treatment Efficacy ◽  
Chronic Wounds ◽  
Therapeutic Potential ◽  
Early Application ◽  
Study Results ◽  
Very High

Amniotic membrane grafts have some therapeutic potential for wounds healing. Early application of amniotic membrane turned out as beneficial in healing ulcers, burns, and dermal injuries. Since the second half of the 20th century, the autotransplants of amniotic/chorion tissue have been also used for the treatment of chronic neuropathic wounds, cornea surface injuries, pterygium and conjunctivochalasis, and dental and neurosurgical applications. The aim of this publication is to prepare a coherent overview of amniotic membrane derivatives use in the field of wound healing and also its efficacy. In total 60 publications and 39 posters from 2000–2020 were examined. In these examined publications of case studies with known study results was an assemblage of 1141 patients, and from this assemblage 977 were successfully cured. In case of posters, the assemblage is 570 patients and 513 successfully cured. From the investigated data it is clear that the treatment efficacy is very high—86% and 90%, respectively. Based on this information the use of the amniotic membrane for chronic wounds can be considered highly effective.

Amniotic membrane application for the healing of chronic wounds and ulcers

Placenta ◽  
2017 ◽  
Vol 59 ◽  
pp. 146-153 ◽  
Author(s):  
Gregorio Castellanos ◽  
Ángel Bernabé-García ◽  
José M. Moraleda ◽  
Francisco J. Nicolás
Keyword(s):  
Amniotic Membrane ◽  
Chronic Wounds

Use of Dehydrated Human Amnion/Chorion Membrane Allografts in More Than 100 Patients with Six Major Types of Refractory Nonhealing Wounds

2018 ◽  
Vol 108 (2) ◽  
pp. 84-89 ◽  
Author(s):  
Matthew Garoufalis ◽  
Darshan Nagesh ◽  
Patrick J. Sanchez ◽  
Robin Lenz ◽  
Sarah J. Park ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Chronic Wounds ◽  
Wound Care ◽  
Biochemical Properties ◽  
Complete Healing ◽  
Human Amnion ◽  
Tissue Healing ◽  
Surgical Wounds ◽  
History Of ◽  
Care Practices

Background: Biochemical properties of the amniotic membrane help modulate inflammation and enhance soft-tissue healing. In controlled trials, the efficacy of dehydrated human amnion/chorion membrane (dHACM) allografts has been established. Our purpose is to describe our experience with using dHACM to treat nonhealing wounds of various etiologies. Methods: We conducted a retrospective review of deidentified data from 117 consecutive patients treated in an outpatient clinic with dHACM allografts with wounds of various etiologies over 2 years. The decision to use advanced wound-care treatments is based on rate of healing observed after initiation of standard wound care and patient risk factors. Eligibility for treatments such as amniotic membrane allografts includes wounds without 50% reduction after 4 weeks, or earlier in patients deemed to be at high risk for nonhealing or with a history of chronic wounds. In micronized or sheet formulation, dHACM is applied to the wound weekly after sharp/mechanical debridement as necessary, and wound-care practices appropriate for wound type and location are continued. Results: Thirty-four percent of allograft recipients had diabetic foot ulcers, 25% had venous leg ulcers, 20% had surgical wounds, 14% had pressure ulcers, 6% had ischemic wounds, and 2% had traumatic wounds. Complete healing occurred in 91.1% of treated patients, with a mean ± SD number of weekly applications per healed wound of 5.1 ± 4.2. Conclusions: In addition to wounds of diabetic origin, dHACM can significantly expedite healing in refractory wounds of varying etiologies.

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