scholarly journals Induced Mutation: Creating Genetic Diversity in Plants

Author(s):  
Kamile Ulukapi ◽  
Ayse Gul Nasircilar
2021 ◽  
Vol 5 (2) ◽  
pp. 89-99
Author(s):  
Kunto Wibisono ◽  
Syarifah Iis Aisyah ◽  
Waras Nurcholis ◽  
Sri Suhesti

Genetic enhancement in vegetatively propagated crops can be done through mutation induction. Colchicine-induced mutation is one of the methods that can be employed to increase plant genetic diversity. This study aimed to determine the effect of colchicine on the performance and genetic parameters of MV3 generation of Plectranthus amboinicus (L.). This study was conducted at the Laboratory of Agricultural Seed Management, Plantation Research and Development Center, from June 2018 to June 2019. Nodes and shoots were used as explants. Mutation induction was performed using colchicine at concentrations of 0%, 0.02%, 0.04%, and 0.06%. Explant regeneration and subculture were done on MS0 medium. The number of plantlets yielded were 59 (0% concentration of colchicine), 60 (0.02%), 81 (0.04%), and 80 plantlets (0.06%), respectively. Results indicated that colchicine-induced mutation in an in vitro culture was able to generate high genetic diversity in both quantitative and qualitative characters of the plantlets. At the concentration of 0.04%, colchicine produced the highest frequency of putative mutants (28.4%). Genetic parameters in MV3 generation of P. amboinicus plantlets showed that five quantitative characters, i.e. plantlet height, number of leaves, number of shoots, leaf length, and number of roots had high heritability values at a concentration around the LC50 value (0.0275%).


2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Siti Hartati Yusida Saragih

Sunflower (Helianthus annus L.) is one of the ornamental plant which is economically. It is potential because of oil contains and has the beautiful flower. Induced mutation is important to get genetic diversity of Sunflower. Mutations are genetic changes in either a single cell or a number of genes or chromosomal changes that can cause somaclonal variations. This research was aimed to find the LD20 and LD50 and to identify genetic diversity of Sunflower with the dosage 0, 150, 200, 250 and 300 Gy. The results showed that the higher LD20 and LD50 was at 158.338 gy and 260.339 gy. The gamma ray iradiation was induce variation and affected significantly toward quantitative character of plant height. Key words: diversity, induced mutation, iradiation, LD20, LD50


Nature India ◽  
2007 ◽  
Author(s):  
Subhra Priyadarshini

1997 ◽  
Vol 56 (1-3) ◽  
pp. 233
Author(s):  
N Mehra

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
YH Kim ◽  
JA Ryuk ◽  
BS Ko ◽  
JW Lee ◽  
SE Oh ◽  
...  

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Shinde ◽  
V Shinde ◽  
J Kurane ◽  
A Harsulkar ◽  
K Mahadik

2019 ◽  
Author(s):  
EV Avramidou ◽  
E Sarrou ◽  
P Papaporfiriou ◽  
E Abraham
Keyword(s):  

Author(s):  
AK Choudhury ◽  
MA Karim ◽  
QA Khaliq ◽  
MM Haque ◽  
JU Ahmed ◽  
...  

2018 ◽  
Vol 30 (2) ◽  
pp. 19-28
Author(s):  
A. J. Oludare ◽  
J. I. Kioko ◽  
A. A. Akeem ◽  
A. T. Olumide ◽  
K. R. Justina ◽  
...  

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.)  obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn


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