scholarly journals Cartilage Tissue Engineering Using Self-Assembling Peptides Composite Scaffolds

2019 ◽  
Author(s):  
Nausika Betriu ◽  
Carlos E. Semino
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
Self Assembling

Cartilage tissue engineering using a new self-assembling peptide gel scaffold

2002 ◽  
pp. 423-428
Author(s):  
John D. Kisiday ◽  
Moonsoo Jin ◽  
Bodo Kurz ◽  
Han-Hwa Hung ◽  
Carlos Semino ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Self Assembling

Composite scaffolds for cartilage tissue engineering

Biorheology ◽  
2008 ◽  
Vol 45 (3-4) ◽  
pp. 501-512 ◽  
Author(s):  
Franklin T. Moutos ◽  
Farshid Guilak
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Composite Scaffolds

scholarly journals Tissue Engineering of Canine Cartilage from Surgically Debrided Osteochondritis Dissecans Fragments

2021 ◽  
Author(s):  
Natalia Vapniarsky ◽  
Lilia Moncada ◽  
Carissa Garrity ◽  
Alice Wong ◽  
Barbro Filliquist ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Thyroid Hormone ◽  
Cartilage Tissue Engineering ◽  
Collagen Content ◽  
Cartilage Tissue ◽  
Mechanical Stimuli ◽  
Primary Chondrocytes ◽  
Native Tissue ◽  
Tissue Constructs ◽  
Self Assembling

AbstractThis study in dogs explored the feasibility of using cartilage fragments removed and discarded during routine palliative surgery for osteochondritis dissecans (OCD) as a source of primary chondrocytes for scaffold-free cartilage tissue-engineering. Primary chondrocytes were obtained from three OCD donors and one age-matched healthy articular cartilage (HAC) donor. After monolayer expansion of primary cells, a three-dimensional spherical suspension culture was implemented. Following this stage, cells were seeded at a high density into custom-made agarose molds that allowed for size and shape-specific constructs to be generated via a method of cellular self-assembling in a scaffold-free environment. Fifty-eight neocartilage constructs were tissue-engineered using this methodology. Neocartilage constructs and native cartilage from shoulder joint were subjected to histological, mechanical, and biochemical testing. OCD and HAC chondrocytes-sourced constructs had uniformly flat morphology and histology consistent with cartilage tissue. Constructs sourced from OCD chondrocytes were 1.5-times (32%) stiffer in compression and 1.3 times (23%) stronger in tension than constructs sourced from HAC chondrocytes and only 8.7-times (81%) less stiff in tension than native tissue. Constructs from both cell sources consistently had lower collagen content than native tissue (22.9%/dry weight [DW] for OCD and 4.1%/DW for HAC vs. 51.1%/DW native tissue). To improve the collagen content and mechanical properties of neocartilage, biological and mechanical stimuli, and thyroid hormone (tri-iodothyronine) were applied to the chondrocytes during the self-assembling stage in two separate studies. A 2.6-fold (62%) increase in compressive stiffness was detected with supplementation of biological stimuli alone and 5-fold (81%) increase with combined biological and mechanical stimuli at 20% strain. Application of thyroid hormone improved collagen content (1.7-times, 33%), tensile strength (1.8-times, 43%), and stiffness (1.3-times, 21%) of constructs, relative to untreated controls. Collectively, these data suggest that OCD chondrocytes can serve as a reliable cell source for cartilage tissue-engineering and that canine chondrocytes respond favorably to biological and mechanical stimuli that have been shown effective in chondrocytes from other animal species, including humans.

scholarly journals PLGA/COL I Composite Scaffold Printed by a Low-temperature Deposition Manufacturing Technique for Application to Cartilage Tissue Engineering

2020 ◽  
Author(s):  
Liangquan Peng ◽  
Yong He ◽  
Weimin Zhu ◽  
Wei Lu ◽  
Yong Huang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Low Temperature ◽  
Composite Scaffold ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
Low Temperature Deposition ◽  
Temperature Deposition

Abstract Background Composite scaffolds of poly(lactic-co-glycolic acid) (PLGA) and PLGA/COL I were developed by a low-temperature deposition manufacturing (LDM) technique using three-dimensional printing technology. Their physical properties were tested, and the scaffolds were then used as cell culture platforms to prepare an ideal scaffold for cartilage tissue engineering. Methods The LDM technique was used to fabricate PLGA and PLGA/COL I composite scaffolds. The macrostructure, micromorphology, porosity, hydrophobicity, mechanical properties, and chemical structure of these scaffolds were examined. Primary chondrocytes were isolated and identified, second-passage cells were seeded onto the two scaffolds, and the adhesion and proliferation of the cells were determined. Results Both the PLGA and PLGA/COL I scaffolds prepared by LDM displayed a regular three-dimensional structure with high porosity. The PLGA scaffold had better mechanical properties than the PLGA/COL I scaffold, while the latter had significantly higher hydrophilicity than the former. The PLGA/COL I scaffold cultured with chondrocytes exhibited a higher adhesion rate and proliferation rate than the PLGA/COL I scaffold. Conclusion The novel PLGA/COL I composite scaffold printed by the LDM technique exhibited favourable biocompatibility and biomechanical characteristics and could be a good candidate for cartilage tissue engineering.

scholarly journals 3D-Printed PCL Composite Scaffolds Incorporating IGF-1 Loaded PLGA Nanoparticles for Cartilage Tissue Engineering

2020 ◽  
Author(s):  
Peiran Wei ◽  
Yan Xu ◽  
Yue Gu ◽  
Qingqiang Yao ◽  
Jiayi Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Release Kinetics ◽  
Cartilage Tissue Engineering ◽  
Plga Nanoparticles ◽  
Contact Angles ◽  
Expression Level ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
3D Printed

Abstract Objective: To fabricate and test a 3D-printed PCL scaffold incorporating IGF-1 loaded PLGA nanoparticles for cartilage tissue engineering.Methods: IGF-1 loaded PLGA nanoparticles were produced by the double-emulsion method, and were incorporated onto 3D printed PCL scaffolds via PDA. Particle size, loading effciency (LE) and encapsulation effciency (EE) of the nanoparticles were examined. SEM, pore size, porosity, compression testing, contact angle, IGF-1 release kinetics of the composite scaffolds were also determined. For cell culture studies, CCK-8, Live/dead, MTT, GAG content and expression level of chondrocytes specific genes and HIF-1α were also tested.Results: There was no difference of the nanoparticle size. And the LE and EE of IGF-1 in PLGA nanoparticles was about 5.53%±0.12% and 61.26%±2.71%, respectively. There was a slower, sustained release for all drug-loaded nanoparticles PLGA/PDA/PCL scaffolds. There was no difference of pore size, porosity, compressive strength of each scaffold. The contact angles PCL scaffolds were significant decreased when coated with PDA and PLGA nanoparticales. (P < 0.05) Live/dead staining showed more cells attached to the IGF-1 PLGA/PDA/PCL scaffolds. The CCK-8 and MTT assay showed higher cell proliferation and better biocompatibility of the IGF-1 PLGA/PDA/PCL scaffolds. (P < 0.05) GAG content, chondrogenic gene expression level of SOX-9, COL-II, N-cadh, ACAN, and HIF pathway related gene(HIF-1α) were significantly higher in IGF-1 PLGA/PDA/PCL scaffolds on days 7 and 14 compared to other groups. (P < 0.05)Conclusions: IGF-1 PLGA/PDA/PCL scaffolds may be a better method for sustained IGF-1 administration and a promising scaffold for cartilage tissue engineering.

scholarly journals Hybrid and Composite Scaffolds Based on Extracellular Matrices for Cartilage Tissue Engineering

2019 ◽  
Vol 25 (3) ◽  
pp. 202-224 ◽  
Author(s):  
Mohsen Setayeshmehr ◽  
Ebrahim Esfandiari ◽  
Mohammad Rafieinia ◽  
Batool Hashemibeni ◽  
Asghar Taheri-Kafrani ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Extracellular Matrices ◽  
Cartilage Tissue ◽  
Composite Scaffolds

Designer functionalised self-assembling peptide nanofibre scaffolds for cartilage tissue engineering

2014 ◽  
Vol 16 ◽  
Author(s):  
Bin He ◽  
Xiao Yuan ◽  
Aiguo Zhou ◽  
Hua Zhang ◽  
Dianming Jiang
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Rational Design ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Regenerative Capacity ◽  
Self Assembling ◽  
Important Approach ◽  
Biomaterial Scaffolds

Owing to the limited regenerative capacity of cartilage tissue, cartilage repair remains a challenge in clinical treatment. Tissue engineering has emerged as a promising and important approach to repair cartilage defects. It is well known that material scaffolds are regarded as a fundamental element of tissue engineering. Novel biomaterial scaffolds formed by self-assembling peptides consist of nanofibre networks highly resembling natural extracellular matrices, and their fabrication is based on the principle of molecular self-assembly. Indeed, peptide nanofibre scaffolds have obtained much progress in repairing various damaged tissues (e.g. cartilage, bone, nerve, heart and blood vessel). This review outlines the rational design of peptide nanofibre scaffolds and their potential in cartilage tissue engineering.

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