scholarly journals Effect of centrifugation on tryptic protein digestion

2017 ◽  
Vol 30 (2) ◽  
pp. 96-101 ◽  
Author(s):  
Soohwan Kim ◽  
Yeoseon Kim ◽  
Dabin Lee ◽  
Inyoung Kim ◽  
Jihyun Paek ◽  
...  
Keyword(s):  
Protein Digestion

Physicochemical characteristics, ATR-FTIR molecular interactions and in vitro starch and protein digestion of thermally-treated whole pulse flours

2018 ◽  
Vol 105 ◽  
pp. 371-383 ◽  
Author(s):  
Carolina Estefanía Chávez-Murillo ◽  
Jorge Ivan Veyna-Torres ◽  
Luisa María Cavazos-Tamez ◽  
Julián de la Rosa-Millán ◽  
Sergio Othon Serna-Saldívar
Keyword(s):  
Molecular Interactions ◽  
Physicochemical Characteristics ◽  
Protein Digestion ◽  
Thermally Treated

On Protein Digestion in Achlorhydria

2009 ◽  
Vol 159 (2) ◽  
pp. 137-139
Author(s):  
PEKKA BRUMMER ◽  
MAIJA YLI-POHJA
Keyword(s):  
Protein Digestion

scholarly journals Changes on proteomic and metabolomic profile in serum of mice induced by chronic exposure to tramadol

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shukun Jiang ◽  
Guojie Liu ◽  
Huiya Yuan ◽  
Enyu Xu ◽  
Wei Xia ◽  
...  
Keyword(s):  
Chronic Exposure ◽  
Molecular Mechanisms ◽  
Fatty Acid Biosynthesis ◽  
Serum Proteins ◽  
Enzyme Inhibitor ◽  
Bile Secretion ◽  
Control Group ◽  
Protein Digestion ◽  
Metabolomic Profile ◽  
Apolipoprotein C

AbstractTramadol is an opioid used as an analgesic for treating moderate or severe pain. The long-term use of tramadol can induce several adverse effects. The toxicological mechanism of tramadol abuse is unclear. Limited literature available indicates the change of proteomic profile after chronic exposure to tramadol. In this study, we analyzed the proteomic and metabolomic profile by TMT-labeled quantitative proteomics and untargeted metabolomics between the tramadol and the control group. Proteomic analysis revealed 31 differential expressed serum proteins (9 increased and 22 decreased) in tramadol-treated mice (oral, 50 mg/kg, 5 weeks) as compared with the control ones. Bioinformatics analysis showed that the dysregulated proteins mainly included: enzyme inhibitor-associated proteins (i.e. apolipoprotein C-III (Apoc-III), alpha-1-antitrypsin 1–2 (Serpina 1b), apolipoprotein C-II (Apoc-II), plasma protease C1 inhibitor, inter-alpha-trypsin inhibitor heavy chain H3 (itih3)); mitochondria-related proteins (i.e. 14-3-3 protein zeta/delta (YWHAZ)); cytoskeleton proteins (i.e. tubulin alpha-4A chain (TUBA4A), vinculin (Vcl)). And we found that the differential expressed proteins mainly involved in the pathway of the protein digestion and absorption. Metabolomics analysis revealed that differential expressed metabolites mainly involved in protein ingestion and absorption, fatty acid biosynthesis, steroid hormone biosynthesis and bile secretion. Our overall findings revealed that chronic exposure to tramadol changed the proteomic and metabolomic profile of mice. Moreover, integrated proteomic and metabolomic revealed that the protein digestion and absorption is the common enrichment KEGG pathway. Thus, the combination of proteomics and metabolomics opens new avenues for the research of the molecular mechanisms of tramadol toxicity.

scholarly journals Immobilization of Trypsin onto Porous Methacrylate-Based Monolith for Flow-through Protein Digestion and Its Potential Application to Chiral Separation Using Liquid Chromatography

Heliyon ◽  
2021 ◽  
pp. e07707
Author(s):  
Suci Amalia ◽  
Stevin Carolius Angga ◽  
Elvina Dhiaul Iftitah ◽  
Dias Septiana ◽  
Baiq Octaviana D. Anggraeny ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Chiral Separation ◽  
Potential Application ◽  
Protein Digestion ◽  
Flow Through

Protein Digestion

2015 ◽  
pp. 121-176 ◽  
Author(s):  
Jean Twombly Snook
Keyword(s):  
Protein Digestion

scholarly journals Intestinal Enterokinase Deficiency: A Newly-recognized Disorder of Protein Digestion

1970 ◽  
Vol 45 (243) ◽  
pp. 651-655 ◽  
Author(s):  
M. J. Tarlow ◽  
B. Hadorn ◽  
M. W. Arthurton ◽  
J. K. Lloyd
Keyword(s):  
Protein Digestion

Acid and rennet gels exhibit strong differences in the kinetics of milk protein digestion and amino acid bioavailability

2014 ◽  
Vol 143 ◽  
pp. 1-8 ◽  
Author(s):  
Florence Barbé ◽  
Olivia Ménard ◽  
Yann Le Gouar ◽  
Caroline Buffière ◽  
Marie-Hélène Famelart ◽  
...  
Keyword(s):  
Amino Acid ◽  
Milk Protein ◽  
Protein Digestion ◽  
Kinetics Of

Development of Luminal Protein Digestion

1989 ◽  
Vol 9 (2) ◽  
pp. 144-162 ◽  
Author(s):  
John R. Britton ◽  
Otakar Koldovsky
Keyword(s):  
Protein Digestion
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