scholarly journals Molecular Investigation of Etiologic Agents Causing Vulvovaginal Candidiasis

2020 ◽  
Vol 13 (8) ◽  
Author(s):  
Shirin Farahyar ◽  
Zahra Ghahri Mobaser ◽  
Elham Razmjou ◽  
Maryam Roudbary ◽  
Maryam Rahimi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Large Subunit ◽  
Vulvovaginal Candidiasis ◽  
Rrna Gene ◽  
Vaginal Secretion ◽  
Pichia Kudriavzevii ◽  
Molecular Approaches ◽  
Etiologic Agents ◽  
Molecular Investigation ◽  
Large Subunit Rrna

Background: Vulvovaginal candidiasis (VVC) is an ordinary infection caused by Candida species. Meanwhile, a shift towards non-albicans Candida (NAC) species has been detected in VVC patients. Objectives: This study aimed at molecular identification of Candida isolates, causing VVC. Methods: Vaginal secretion samples of 320 non-pregnant vaginitis patients at Shahid Akbar-Abadi Obstetrics and Gynecology Hospital in Tehran (Iran) were collected. Samples were evaluated using mycological and molecular approaches. Vaginitis isolates were analyzed with the PCR using NL1 and NL4 primers, and the D1/D2 region of the large-subunit rRNA gene was amplified and sequenced. Results: In total, 100 Candida isolates were identified from VVC and recurrent vulvovaginal candidiasis (RVVC). Candida albicans was the most frequent (51%), followed by C. glabrata (36%), C. krusei (Pichia kudriavzevii) (8%), and C. kefyr (Kluyveromyces marxianus) (5%). 51 and 49% of isolates had C. albicans and NAC, respectively. Conclusions: Candida albicans and C. glabrata were the most common agents of vulvovaginal candidiasis. NAC spp. (49%) was found as an important agent associated with VVC.

scholarly journals Candida saraburiensis sp. nov. and Candida prachuapensis sp. nov., xylose-utilizing yeast species isolated in Thailand

2011 ◽  
Vol 61 (2) ◽  
pp. 462-468 ◽  
Author(s):  
Sukanya Nitiyon ◽  
Chanita Boonmak ◽  
Somjit Am-In ◽  
Sasitorn Jindamorakot ◽  
Hiroko Kawasaki ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Estuarine Water ◽  
D2 Domain ◽  
Large Subunit Rrna

Four strains of two novel xylose-utilizing yeast species were obtained from samples collected in Thailand from decaying corncobs (strains KU-Xs13T and KU-Xs18), a decaying grass (KU-Xs20) and estuarine water from a mangrove forest (WB15T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 domain of the large subunit rRNA gene, the four strains were found to represent two novel species of the genus Candida in the Candida albicans/Lodderomyces elongisporus clade. Three strains (KU-Xs13T, KU-Xs18 and KU-Xs20) were assigned as a single novel species, which was named Candida saraburiensis sp. nov. The type strain is KU-Xs13T (=CBS 11696T=NBRC 106721T=BCC 39601T). Strain WB15T represented another novel species of the genus Candida that was named Candida prachuapensis sp. nov. The type strain is WB15T (=CBS 11024T=NBRC 104881T=BCC 29904T).

scholarly journals Sympodiomyces attinorum sp. nov., a yeast species associated with nests of the leaf-cutting ant Atta sexdens

2004 ◽  
Vol 54 (5) ◽  
pp. 1891-1894 ◽  
Author(s):  
Solange C. Carreiro ◽  
Fernando C. Pagnocca ◽  
Maurício Bacci ◽  
Marc-André Lachance ◽  
Odair C. Bueno ◽  
...  
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Growth Characteristics ◽  
Rrna Gene ◽  
The Novel ◽  
Atta Sexdens ◽  
Leaf Cutting ◽  
Large Subunit Rrna ◽  
Waste Deposit

Four strains of a novel yeast species were isolated from laboratory nests of the leaf-cutting ant Atta sexdens in Brazil. Three strains were found in older sponges and one was in a waste deposit in the ant nests. Sequencing of the D1/D2 region of the large-subunit rRNA gene showed that the novel species, named Sympodiomyces attinorum sp. nov., is phylogenetically related to Sympodiomyces parvus. Unlike Sympodiomyces parvus, Sympodiomyces attinorum can ferment glucose, assimilate methyl α-d-glucoside, salicin and citrate, and grow at 37 °C, thus enabling these two species to be distinguished. Differentiation from other related species is possible on the basis of other growth characteristics. The type strain of Sympodiomyces attinorum is UNESP-S156T (=CBS 9734T=NRRL Y-27639T).

scholarly journals Detection of Pneumocystis carinii DNA in Blood Specimens from Human Immunodeficiency Virus-Infected Patients by Nested PCR

1999 ◽  
Vol 37 (1) ◽  
pp. 127-131 ◽  
Author(s):  
Meja Rabodonirina ◽  
Laurent Cotte ◽  
André Boibieux ◽  
Karine Kaiser ◽  
Martine Mayençon ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Whole Blood ◽  
Nested Pcr ◽  
Large Subunit ◽  
Pneumocystis Carinii ◽  
Proteinase K ◽  
Rrna Gene ◽  
Immunodeficiency Virus ◽  
Blood Specimens ◽  
Large Subunit Rrna

The detection of Pneumocystis carinii DNA in blood by PCR could be useful for studying the natural history of pneumocystosis and could also be a noninvasive diagnostic method. The results of previous studies are nevertheless conflicting. In our study, we compared three commercially available DNA extraction kits (GeneReleaser, QIAamp Tissue Kit, and ReadyAmp Genomic DNA Purification System) and proteinase K and proteinase K-phenol-chloroform treatments for the extraction of P. carinii DNA from dilutions of a P. carinii f. sp.hominis cyst suspension mixed with human whole blood. A rapid and simple nested PCR protocol which amplifies a portion of the mitochondrial large-subunit rRNA gene was applied to all the extraction products. The QIAmp Tissue Kit was the most effective kit for the isolation of amplification-ready P. carinii DNA and was used with nested PCR for the testing of whole-blood specimens from 35 immunocompetent control patients and 84 human immunodeficiency virus (HIV)-infected patients investigated for pulmonary disease and/or fever. In HIV-infected patients, P. carinii DNA was detected by nested PCR in blood samples from 3 of 14 patients with microscopically proven P. carinii pneumonia, 7 of 22 patients who were considered to be colonized with P. carinii, and 9 of 48 patients who were neither infected nor colonized with P. carinii. P. carinii DNA was not detected in blood specimens from the 35 immunocompetent patients. P. carinii DNA in blood might represent viable P. carinii organisms or DNA complexes released from pulmonary phagocytes. In conclusion, P. carinii DNA may be detected in whole blood from HIV-infected patients, but the nature and the meaning of the circulating form of P. carinii remain to be established.

scholarly journals Assessment of Diversity of Culturable Marine Yeasts Associated with Corals and Zoanthids in the Gulf of Thailand, South China Sea

2020 ◽  
Vol 8 (4) ◽  
pp. 474 ◽  
Author(s):  
Chutima Kaewkrajay ◽  
Thanongsak Chanmethakul ◽  
Savitree Limtong
Keyword(s):  
Marine Invertebrates ◽  
Large Subunit ◽  
Rrna Gene ◽  
Gulf Of Thailand ◽  
Marine Yeasts ◽  
D2 Domain ◽  
Wide Range ◽  
The Gulf Of Thailand ◽  
Ascomycetous Yeasts ◽  
Large Subunit Rrna

Marine yeasts can occur in a wide range of habitats, including in marine invertebrates, in which they may play important roles; however, investigation of marine yeasts in marine invertebrates is scarce. Therefore, this study aims to explore the diversity of yeasts associated with corals and zoanthids in the Gulf of Thailand. Thirty-three coral and seven zoanthid samples were collected at two sampling sites near Mu and Khram islands. Fifty yeast strains were able to be isolated from 25 of the 40 samples collected. Identification based on sequence analyses of the D1/D2 domain of the large subunit rRNA gene revealed a higher number of strains in the phylum Basidiomycota (68%) than in the phylum Ascomycota. The ascomycetous yeasts comprised nine known species from four genera (Candida, Meyerozyma, Kodamaea, and Wickerhamomyces), whereas the basidiomycetous yeasts comprised 10 known species from eight genera (Vishniacozyma, Filobasidium, Naganishia, Papiliotrema, Sterigmatomyces, Cystobasidium, Rhodotorula, and Rhodosporidiobolus) and one potentially new species. The species with the highest occurrence was Rhodotorula mucilaginosa. Using principal coordinate analysis (PCoA) ordination, no marked differences were found in the yeast communities from the two sampling sites. The estimation of the expected richness of species was higher than the actual richness of species observed.

Schistonchus hirtus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta in China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 543-556 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Robin M. Giblin-Davis ◽  
Changhui Li ◽  
Zhijian Du ◽  
...  
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characters ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Wasp Species ◽  
Dna Sequence Data ◽  
Ficus Hirta ◽  
Large Subunit Rrna

Abstract A nematode recovered from syconia of Ficus hirta from Guangzhou, P. R. China, during a survey of nematode biodiversity from 2007 to 2009, is described herein as Schistonchus hirtus n. sp. and is differentiated by a combination of morphological characters, including excretory pore (EP) located near the metacorpus, a short post-uterine sac (PUS) (0.5 vulval body diam. (VBD) long), rose thorn-shaped spicules, amoeboid sperm, absence of gubernaculum, three pairs of subventral papillae on the male tail, host-Ficus and host-wasp species and DNA sequence data. Morphologically, S. hirtus n. sp. is close to S. centerae, S. altermacrophylla, S. aureus, S. laevigatus and S. virens based upon the length of the PUS (about 0.5 VBD long). However, the relative position of the EP in S. hirtus n. sp. is very different from these species (near metacorpus vs near head). With regard to the EP character, S. hirtus n. sp. is very similar to S. macrophylla, S. guangzhouensis and S. caprifici where the EP is at metacorpus level. However, S. hirtus n. sp. differs from S. macrophylla and S. guangzhouensis by possessing a shorter PUS and smaller spicules, and differs from S. caprifici by a shorter female stylet and smaller spicules. Schistonchus hirtus n. sp. was easily differentiated from other sequenced species by the proportion of parsimony informative changes in the partial small subunit rRNA gene (SSU) and D2/D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with SSU sequences suggests that S. hirtus n. sp. is in a highly supported monophyletic clade with Aphelenchoides and Laimaphelenchus and is polyphyletic to other sequenced Schistonchus species. With LSU sequence data, it forms a clade with S. caprifici and they appear polyphyletic relative to S. guangzhouensis, S. centerae, S. aureus, S. laevigatus and S. virens.

Wickerhamomyces ochangensis sp. nov., an ascomycetous yeast isolated from the soil of a potato field

2011 ◽  
Vol 61 (10) ◽  
pp. 2543-2546 ◽  
Author(s):  
Kee-Sun Shin ◽  
Kyung-Sook Bae ◽  
Kang Hyun Lee ◽  
Doo-Sang Park ◽  
Gi-Seok Kwon ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Potato Field ◽  
Rrna Gene Sequence ◽  
Ascomycetous Yeast ◽  
D2 Domain ◽  
26S Rrna ◽  
Large Subunit Rrna

A novel ascomycetous yeast, designated strain N7a-Y2T, was isolated from soil collected in a potato field in Ochang, Korea, and its taxonomic position was studied. A neighbour-joining tree based on the D1/D2 domain of large-subunit rRNA gene sequences revealed that the isolate was a member of the Wickerhamomyces clade and that it was closely related to Wickerhamomyces bisporus, Candida quercuum, Candida ulmi and Wickerhamomyces alni. Strain N7a-Y2T formed Saturn-shaped ascospores in unconjugated and persistent asci. D1/D2 domain 26S rRNA gene sequence divergences of 11.0–21.1 % between strain N7a-Y2T and other members of the Wickerhamomyces clade indicate that the strain represents a novel species of the genus Wickerhamomyces, for which the name Wickerhamomyces ochangensis sp. nov. is proposed. The type strain is N7a-Y2T ( = KCTC 17870T  = CBS 11843T).

Candida ficus sp. nov., a novel yeast species from the gut of Apriona germari larvae

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2805-2809 ◽  
Author(s):  
Feng-Li Hui ◽  
Qiu-Hong Niu ◽  
Tao Ke ◽  
Zheng Liu
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Central China ◽  
Rrna Gene ◽  
The Novel ◽  
Ascomycetous Yeasts ◽  
Large Subunit Rrna ◽  
Wood Boring

A novel yeast species is described based on three strains from the gut of wood-boring larvae collected in a tree trunk of Ficus carica cultivated in parks near Nanyang, central China. Phylogenetic analysis based on sequences of the D1/D2 domains of the large subunit rRNA gene showed that these strains occurred in a separate clade that was genetically distinct from all known ascomycetous yeasts. In terms of pairwise sequence divergence, the novel strains differed by 15.3 % divergence from the type strain of Pichia terricola, and by 15.8 % divergence from the type strains of Pichia exigua and Candida rugopelliculosa in the D1/D2 domains. All three are ascomycetous yeasts in the Pichia clade. Unlike P. terricola, P. exigua and C. rugopelliculosa, the novel isolates did not ferment glucose. The name Candida ficus sp. nov. is proposed to accommodate these highly divergent organisms, with STN-8T ( = CICC 1980T = CBS 12638T) as the type strain.

scholarly journals Rearranged Subtelomeric rRNA Genes in Giardia duodenalis

2005 ◽  
Vol 4 (2) ◽  
pp. 484-486 ◽  
Author(s):  
Jacqueline A. Upcroft ◽  
Mahin Abedinia ◽  
Peter Upcroft
Keyword(s):  
Large Subunit ◽  
Giardia Duodenalis ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Two Dimensional ◽  
Linear Chromosomes ◽  
Large Subunit Rrna

ABSTRACT Giardia duodenalis has linear chromosomes capped with typical eukaryotic repeats [(TAGGG) n ], subtelomeric rRNA genes, and telomere gene units. The absence of two closely associated NotI sites in the large-subunit rRNA gene was used as an indicator in hybridizations of one- and two-dimensional NotI-cleaved Giardia chromosome separations that some chromosomes carry only rearranged and, by deduction, nonfunctional rRNA genes.

scholarly journals Candida andamanensis sp. nov., Candida laemsonensis sp. nov. and Candida ranongensis sp. nov., anamorphic yeast species isolated from estuarine waters in a Thai mangrove forest

2011 ◽  
Vol 61 (2) ◽  
pp. 454-461 ◽  
Author(s):  
Somjit Am-In ◽  
Savitree Limtong ◽  
Wichien Yongmanitchai ◽  
Sasitorn Jindamorakot
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
D2 Domain ◽  
Large Subunit Rrna

Five strains (RV5T, RV140, R31T, RS17 and RS28T) representing three novel anamorphic ascomycetous yeast species were isolated by membrane filtration from estuarine waters collected from a mangrove forest in Laem Son National Park, Ranong Province, Thailand, on different occasions. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and the internal transcribed spacer region and phylogenetic analysis, three strains were found to represent two novel Candida species. Two strains (RV5T and RV140) represented a single novel species, for which the name Candida laemsonensis sp. nov. is proposed. The type strain is RV5T (=BCC 35154T =NBRC 105873T =CBS 11419T). Strain R31T was assigned to a novel species that was named Candida andamanensis sp. nov. (type strain R31T =BCC 25965T =NBRC 103862T =CBS 10859T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and phylogenetic analysis, strains RS17 and RS28T represented another novel species of Candida, for which the name Candida ranongensis sp. nov. is proposed. The type strain is RS28T (=BCC 25964T =NBRC 103861T =CBS 10861T).

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