Cytologic and Histologic Findings of Nephroblastoma in a Captive Desert Tortoise (Gopherus agassizii)

2021 ◽  
Author(s):  
Elise LaDouceur ◽  
Michael M. Garner ◽  
Ginger F. Hammett ◽  
Louisa M. Asseo
Keyword(s):  
Wilms Tumor ◽  
Needle Aspiration ◽  
Mesenchymal Cells ◽  
Gopherus Agassizii ◽  
Desert Tortoise ◽  
X Ray ◽  
Immunohistochemical Markers ◽  
S 100 ◽  
History Of ◽  
Cell Proliferations

A 97-year-old, male, captive desert tortoise ( Gopherus agassizii ) had a 2 month history of lethargy. Imaging with ultrasound, X-ray, and computed tomography revealed a 10-cm-diameter mass in the caudal coelom. Fine needle aspiration revealed spindle and epithelial cell proliferations with formation of rosettes. Exploratory surgery was performed and the mass was removed and submitted for pathology. There was no evidence of metastasis on imaging or at surgery. Histology revealed a mass arising from and compressing the kidney. The mass was composed of primitive glomeruli, tubules, blastemal cells, and mesenchymal cells, features that are diagnostic for nephroblastoma. Tubules were reactive to cytokeratin and mesenchymal cells were reactive to desmin via immunohistochemistry; other immunohistochemical markers were either negative (i.e. S-100) or non-contributory (i.e. epithelial membrane antigen, myogenin, vimentin, and Wilms’ Tumor 1). This is the first report of nephroblastoma in a chelonian.

Nodular fasciitis: a case series

2008 ◽  
Vol 123 (5) ◽  
pp. 541-544 ◽  
Author(s):  
S D Reitzen ◽  
S Dogan ◽  
G Har-El
Keyword(s):  
Frozen Section ◽  
Case Series ◽  
Final Diagnosis ◽  
Needle Aspiration ◽  
Neck Mass ◽  
Nodular Fasciitis ◽  
Neurogenic Tumour ◽  
Parotid Tumour ◽  
Immunohistochemical Markers ◽  
History Of

AbstractBackground:Given its rarity, varied histological presentation and often pseudosarcomatous appearance, nodular fasciitis is frequently misdiagnosed on pre-operative, intra-operative and final analyses.Methods:Four cases of nodular fasciitis are reviewed.Results:Physical and radiological findings were consistent with a parapharyngeal tumour, probably neurogenic, a level four neck mass suspicious for lymphoma; a sternoclavicular mass in a patient with a history of breast cancer suspicious for metastasis; and a cheek mass consistent with an accessory parotid tumour. Fine needle aspiration results were consistent with a neurogenic tumour in two patients and an undifferentiated malignancy in two patients. Frozen section examination most commonly included masses with spindle-type cells. The final diagnosis of nodular fasciitis was made only after permanent section and immunohistological analysis.Conclusions:In a patient with nonspecific results following investigation of a head or neck mass, nodular fasciitis should be considered. Use of appropriate immunohistochemical markers will aid in the final diagnosis.

scholarly journals Desmoplastic melanoma morphology on Thinprep: a report of two cases

CytoJournal ◽  
2007 ◽  
Vol 4 ◽  
pp. 18 ◽  
Author(s):  
Becky L Van Ells ◽  
James E Madory ◽  
Rana S Hoda
Keyword(s):  
Needle Aspiration ◽  
Excellent Correlation ◽  
Cytoplasmic Staining ◽  
Case Presentation ◽  
Fibrous Stroma ◽  
Desmoplastic Melanoma ◽  
Spindle Cells ◽  
First Case ◽  
S 100 ◽  
History Of

Background Desmoplastic melanoma is a variant of malignant melanoma that can range in appearance from sarcomatoid to scar-like. Cytomorphology of desmoplastic melanoma has been previously described on conventional smears; however, to our knowledge, detailed cytomorphology on ThinPrep has so far not been described. Herein, we describe the cytomorphology of two cases of desmoplastic melanoma on fine needle aspiration processed as ThinPrep slides and compare it to that seen on conventional smears. Pertinent immunocytochemical stains, performed on ThinPrep slides are also discussed. Case presentation The first case is a woman with a history of desmoplastic melanoma of the scalp with previous local recurrences and lymph node metastasis with a new submandibular mass. The second case is a man with a previously resected desmoplastic melanoma with his first local recurrence. Conventional smears, including air-dried Diff-Quik-stained and alcohol-fixed Papanicolaou-stained smears, demonstrated aggregates of pleomorphic spindle cells admixed with fibrous stroma and single spindle cells. In both cases, nuclei were elongated and plump with irregular nuclear contours, deep grooves, and folds. Chromatin was dark and coarse with either inconspicuous or multiple prominent nucleoli. Cytoplasm was located at the nuclear poles and was fine, wispy, and delicate. The background was clean with no evidence of necrosis or melanin pigment. Papanicolaou-stained ThinPrep slides were prepared from needle rinses and demonstrated excellent correlation of nuclear and cytoplasmic detail of single spindle cells to that seen on conventional smears with the exception of only slight decrease in nuclear size; however, nuclear and cytoplasmic detail of spindle cells embedded in stroma was markedly attenuated. Confirmatory immunostain for S-100 protein in both cases was performed on ThinPrep slides demonstrating crisp cytoplasmic staining in the spindle cells. Conclusion The cytomorphology of desmoplastic melanoma shows excellent correlation between cytomorphology of single spindle cells on conventional smears and on ThinPrep slides. The major difference noted on ThinPrep slides was attenuated nuclear and cytoplasmic detail of spindle cells embedded in fibrous stoma.

scholarly journals Benign solitary fibrous pleural tumor and hypoglycaemia

2007 ◽  
Vol 135 (5-6) ◽  
pp. 326-329 ◽  
Author(s):  
Branislava Milenkovic ◽  
Jelena Stojsic ◽  
Aleksandra Dudvarski ◽  
Vesna Bosnjak-Petrovic ◽  
Maja Ercegovac
Keyword(s):  
Immunohistochemical Analysis ◽  
Needle Aspiration ◽  
Normal Range ◽  
Intravenous Glucose ◽  
Pleural Tumor ◽  
X Ray ◽  
Solitary Fibrous Tumors ◽  
History Of ◽  
Chest X Ray ◽  
One Year

Introduction: Solitary fibrous tumors of the pleura are uncommon and discovered fortuitously or in patients with non-specific respiratory symptoms. When associated with hypoglycaemia, they are commonly referred to as Doege-Potter syndrome. Case outline. A 68-year old woman presented with a large pleural mass. She had a long history of headache and decreased consciousness with one-year worsening dyspnoea and right-sided chest pain. The chest X-ray revealed an enormous opacity occupying almost the entire right hemithorax. Endocrine tests showed an extremely reduced glucose level and blood concentration of insulin, C-peptide, glucagon, growth hormone and catecholamines within normal range. After fine-needle aspiration, by histological and immunohistochemical analysis, a benign solitary fibrous pleural tumor was diagnosed. Although surgical resection of such a huge tumor with hypoglycaemia is usually curative, our patient declined surgery and opted for conservative treatment with intravenous glucose. Conclusion. Clinicians should be aware of this rare, but important cause of hypoglycaemia and exclude a fibrous pleural tumor in the assessment of their patients. .

Lingual Dystonia Treated with Botulinum Toxin - A Case Report

1970 ◽  
Vol 24 (2) ◽  
pp. 75-78
Author(s):  
MA Hayee ◽  
QD Mohammad ◽  
H Rahman ◽  
M Hakim ◽  
SM Kibria
Keyword(s):  
Botulinum Toxin ◽  
Botulinum Toxin A ◽  
Botulinum Toxin Type A ◽  
Botulinum Toxin Type ◽  
X Ray ◽  
Medical College ◽  
Lingual Dystonia ◽  
History Of ◽  
Chest X Ray

A 42-year-old female presented in Neurology Department of Sir Salimullah Medical College with gradually worsening difficulty in talking and eating for the last four months. Examination revealed dystonic tongue, macerated lips due to continuous drooling of saliva and aspirated lungs. She had no history of taking antiparkinsonian, neuroleptics or any other drugs causing dystonia. Chest X-ray revealed aspiration pneumonia corrected later by antibiotics. She was treated with botulinum toxin type-A. Twenty units of toxin was injected in six sites of the tongue. The dystonic tongue became normal by 24 hours. Subsequent 16 weeks follow up showed very good result and the patient now can talk and eat normally. (J Bangladesh Coll Phys Surg 2006; 24: 75-78)

Highlights in the history of X-ray topography1

1995 ◽  
Vol 210 (6) ◽  
Author(s):  
A. R. Lang
Keyword(s):  
Magnetic Domain ◽  
Diffraction Theory ◽  
Dislocation Loops ◽  
X Ray Diffraction ◽  
X Ray ◽  
Domain Structures ◽  
Structure Factors ◽  
History Of ◽  
Dislocation Sources ◽  
Non Destructive

AbstractX-ray topography provides a non-destructive method of mapping point-by-point variations in orientation and reflecting power within crystals. The discovery, made by several workers independently, that in nearly perfect crystals it was possible to detect individual dislocations by X-ray diffraction contrast started an epoch of rapid exploitation of X-ray topography as a new, general method for assessing crystal perfection. Another discovery, that of X-ray Pendellösung, led to important theoretical developments in X-ray diffraction theory and to a new and precise method for measuring structure factors on an absolute scale. Other highlights picked out for mention are studies of Frank-Read dislocation sources, the discovery of long dislocation helices and lines of coaxial dislocation loops in aluminium, of internal magnetic domain structures in Fe-3 wt.% Si, and of stacking faults in silicon and natural diamonds.

scholarly journals Thymic Carcinoid Tumour

2002 ◽  
Vol 9 (3) ◽  
pp. 215-216 ◽  
Author(s):  
Isabel Parra ◽  
Antonia Remacha ◽  
Serafín Costilla ◽  
José Antonio Santos Calderon
Keyword(s):  
Fine Needle Aspiration ◽  
Carcinoid Tumour ◽  
Needle Aspiration ◽  
Thymic Carcinoid ◽  
Fine Needle ◽  
X Ray ◽  
Carcinoid Tumours ◽  
Chest X Ray

Carcinoid tumours of the thymus are rare. The case of a 57-year-old asymptomatic man with a carcinoid tumour of the thymus, who showed a widened mediastinum by chest x-ray, is presented. Fine needle aspiration suggested the diagnosis, which was confirmed by biopsy.

scholarly journals Unusual Tissue – Unusual Issue: Pancreatic Heterotopia Presenting as Gastric Outlet Obstruction

2021 ◽  
pp. 338-343
Author(s):  
Thu L. Nguyen ◽  
Shivani Kapur ◽  
Stephen C. Schlack-Haerer ◽  
Grzegorz T. Gurda ◽  
Milan E. Folkers
Keyword(s):  
Gastric Outlet Obstruction ◽  
Epigastric Pain ◽  
Outlet Obstruction ◽  
Needle Aspiration ◽  
Pancreatic Tissue ◽  
Cystic Mass ◽  
Pancreatic Heterotopia ◽  
History Of

Pancreatic heterotopia (PH) is a common, but typically small (<1 cm), incidental and asymptomatic finding; however, PH should be considered even for large and symptomatic upper gastrointestinal masses. A 27-year-old white woman presented with a 3-week history of burning epigastric pain, nausea, early satiety, and constipation. Physical examination revealed epigastric and right upper quadrant tenderness with normal laboratory workup, but imaging revealed a 5-cm, partly cystic mass arising from the gastric antrum with resulting pyloric stenosis and partial gastric outlet obstruction. Endoscopic ultrasound-guided fine needle aspiration revealed PH – an anomalous pancreatic tissue lying in a nonphysiological site. The patient ultimately underwent a resection and recovered uneventfully, with a complete pathologic examination revealing normal exocrine pancreatic tissue (PH type 2) without malignant transformation. We report a case of heterotopic pancreas manifesting as severe gastric outlet obstruction, in addition to a thorough diagnostic workup and surgical follow-up, in a young adult. Differential diagnoses and features that speak to benignity of a large, symptomatic mass lesion (PH in particular) are discussed.

The effect of research activities and winter precipitation on voiding behaviour of Agassiz’s desert tortoises (Gopherus agassizii)

2014 ◽  
Vol 41 (8) ◽  
pp. 641 ◽  
Author(s):  
Mickey Agha ◽  
Mason O. Murphy ◽  
Jeffrey E. Lovich ◽  
Joshua R. Ennen ◽  
Christian R. Oldham ◽  
...  
Keyword(s):  
Stress Responses ◽  
Handling Time ◽  
Winter Precipitation ◽  
Contact Period ◽  
Gopherus Agassizii ◽  
Desert Tortoise ◽  
Defensive Strategy ◽  
Survivorship Analysis ◽  
Research Activities

Context There is little information available on how research activities might cause stress responses in wildlife, especially responses of threatened species such as the desert tortoise (Gopherus agassizii). Aims The present study aims to detect behavioural effects of researcher handling and winter precipitation on a natural population of desert tortoises in the desert of Southwestern United States, over the period 1997 to 2014, through extensive assessments of capture events during multiple research studies, and capture–mark–recapture survivorship analysis. Methods Juvenile and adult desert tortoises were repeatedly handled with consistent methodology across 18 years during 10 study seasons. Using a generalised linear mixed-effects model, we assessed the effects of both research manipulation and abiotic conditions on probability of voiding. Additionally, we used a Cormack–Jolly–Seber model to assess the effects of winter precipitation and voiding on long-term apparent survivorship. Key results Of 1008 total capture events, voiding was recorded on 83 (8.2%) occasions in 42 different individuals. Our top models indicated that increases in handling time led to significantly higher probabilities of voiding for juveniles, females and males. Similarly, increases in precipitation resulted in significantly higher probabilities of voiding for juveniles and females, but not for males. Tortoise capture frequency was negatively correlated with voiding occurrence. Cormack–Jolly–Seber models demonstrated a weak effect of winter precipitation on survivorship, but a negligible effect for both voiding behaviour and sex. Conclusions Handling-induced voiding by desert tortoises may occur during common research activities and years of above average winter precipitation. Increased likelihood of voiding in individuals with relatively low numbers of recaptures suggested that tortoises may have perceived researchers initially as predators, and therefore voided as a defensive strategy. Voiding does not appear to impact long-term survivorship in desert tortoises at this site. Implications This study has demonstrated that common handling practices on desert tortoise may cause voiding behaviour. These results suggest that in order to minimise undesirable behavioural responses in studied desert tortoise populations, defined procedures or protocols must be followed by the investigators to reduce contact period to the extent feasible.

scholarly journals Predictive Factors of Malignancy in Cytology of Indeterminate Follicular and Hürthle Cell Neoplasms of the Thyroid Gland

2019 ◽  
Vol 103 (1-2) ◽  
pp. 9-14
Author(s):  
Bahadır Öz ◽  
Serap Doğan ◽  
Ertan Emek ◽  
Muhammed Akyüz ◽  
Alper Akcan ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Family History ◽  
Radiation Exposure ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Cytologic Diagnosis ◽  
Fine Needle ◽  
Solitary Nodule ◽  
History Of

The objective of the current study was to determine the risk of malignancy in patients with thyroid nodules with cytology of indeterminate follicular and indeterminate Hürthle cell neoplasm (HN). The cytologic diagnosis of follicular neoplasm (FN) or HN remains a diagnostic challenge. Often, surgery is recommended for such lesions. A retrospective analysis was performed on 80 patients who underwent thyroid surgery following a diagnosis of indeterminate FN and indeterminate HN in thyroid fine-needle aspiration biopsy. Sex; age; family history of thyroid cancer and radiation exposure; coexisting thyroid conditions, such as solitary nodule; multinodularity; cytologic diagnosis; sonographic features; type of surgical treatment; and histopathologic results were recorded. Of the 80 patients, 52 (65%) had FN on fine-needle aspiration biopsy cytology and 28 (35%) had HN. A total of 23 patients (28.7%) had primary thyroid cancers on surgical pathology, and 57 (71.3%) had benign diagnoses. Univariate analysis showed no differences between the benign and malignant groups by sex, nodule size, family history of thyroid cancer, history of radiation exposure, presence of solitary nodule or multinodularity in the nodular features. In multivariate binary logistic regression analysis, the factors that were statistically significant predictors of malignancy were microcalcification [odds ratio (OR), 10.9; 95% confidence interval (CI), 2.18–54.7; P = 0.004], being older than 45 years (OR, 4.2; 95% CI, 1.25–14.63; P = 0.02]. The independent predictors of malignancy in FN and HN are micorcalcification and being older than 45 years, the use of which may predict the risk of thyroid cancer.

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