scholarly journals Comparison of High-Throughput Fully Automated Immunoanalyzers for Detecting Hepatitis B Virus Infection

2019 ◽  
Vol 144 (5) ◽  
pp. 612-619
Author(s):  
Dongju Won ◽  
Younhee Park ◽  
Dasom Choi ◽  
Hyon-Suk Kim
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
High Throughput ◽  
Hepatitis B Surface Antigen ◽  
B Virus ◽  
Positive Rate ◽  
Antibody Levels ◽  
Hepatitis B Viral Infection ◽  
Surface Antibody

Context.— High-throughput automated immunoanalyzers for hepatitis B virus serologic markers have been introduced but have not been compared to existing systems. Objective.— To compare hepatitis B surface antigen, hepatitis B surface antibody, and total hepatitis B core antibody analyses between our Architect i2000 platform and newer high-throughput fully automated immunoanalyzers. Design.— From May to June 2018, a total of 932, 914, and 1055 samples tested for hepatitis B surface antigen, hepatitis B surface antibody, and total hepatitis B core antibody, respectively, with the Architect i2000 system for routine testing in our center were tested again with Alinity i, Atellica IM, and Cobas e801 systems. Results.— Total concordance rates among the systems were 98.0%, 89.5%, and 93.0% for hepatitis B surface antigen, hepatitis B surface antibody, and total hepatitis B core antibody, respectively. Cohen's κ values exceeded 0.8. The correlations between serum hepatitis B surface antibody levels quantified by all 4 systems were high (r > 0.85). The hepatitis B surface antibody averages were greater for the Alinity i, Atellica IM, and Cobas e801 than for the Architect i2000 (P < .001). Conclusions.— Alinity i, Atellica IM, and Cobas e801 automated immunoanalyzers performed well when compared with the existing Architect i2000 system with regard to detection of hepatitis B viral infection. However, the new systems have higher titer and positivity rates for hepatitis B surface antibody and are more sensitive. Notably, the Atellica IM has a lower positive rate for total hepatitis B core antibody than does the Architect i2000.

scholarly journals Binding of biotin to hepatitis B surface antigen: a possible pitfall in immunohistochemistry.

1989 ◽  
Vol 37 (4) ◽  
pp. 551-554 ◽  
Author(s):  
J J van den Oord ◽  
F Facchetti ◽  
C de Wolf-Peeters ◽  
V J Desmet
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Disulfide Bonds ◽  
Hepatitis B Surface Antigen ◽  
B Virus ◽  
Infected Liver ◽  
Hepatitis B Viral Infection ◽  
Positive Results

We report on the binding of biotin, and hence of biotinylated antibodies and lectins, to ground glass hepatocytes and liver cell membranes in chronic hepatitis B viral infection. This binding is of low affinity, and was proved to be directed at the hepatitis B surface antigen, presumably at its disulfide bonds. To avoid false-positive results, this affinity should be considered in the interpretation of immunohistochemical stainings of hepatitis B virus-infected liver tissue with biotinylated reagents.

scholarly journals Serum immunoconglutinin titers during acute and chronic hepatitis B virus infection

1980 ◽  
Vol 28 (3) ◽  
pp. 842-845
Author(s):  
G M Makhdoomi ◽  
M L Tiku ◽  
K R Beutner ◽  
P L Ogra
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Chronic Hepatitis B Virus ◽  
B Virus ◽  
Acute And Chronic Hepatitis ◽  
Surface Antibody

Sera from 99 chronic hepatitis B surface antigen carriers, 12 individuals with acute type B hepatitis, 26 hepatitis B surface antibody-seropositive subjects, and 50 hepatitis B surface antigen, hepatitis B surface antibody-seronegative subjects were evaluated for the presence of serum imunoconglutinis (IKs). The mean serum IK titers of hepatitis B surface antibody-seropositive and hepatitis B virus-seronegative subjects wre 5.3 and 4.9, respectively. The IK titers of subjects with acute and chronic hepatitis B virus infections were 215.4 and 19.1, respectively. These groups also manifested IK titers greater than or equal to > 16 significantly (P < 0.005) more often than controls did. Among chronic hepatitis B surface antigen carriers, high IK titers were associated with low levels of hepatitis B surface antigen. IK titers of individuals chronically infected with hepatitis B virus and having the rheumatoid factor were similar to those of individuals without the rheumatoid factor. Elevated IK titers represent a physiological autoimmune response and may indicate the presence of immune complexes in acute and chronic hepatitis B virus infection.

scholarly journals SEROLOGICAL CHANGES AGAINST HEPATITIS B SURFACE ANTIGEN IN CHILDREN AND ADOLESCENTS RECEIVING CHEMOTHERAPY FOR ACUTE LEUKEMIA

2019 ◽  
Vol 11 (1) ◽  
pp. e2019052
Author(s):  
Seung Beom Han ◽  
Hye Jo Shin ◽  
Eui Soo Lee ◽  
Jae Wook Lee ◽  
Nack-Gyun Chung ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Acute Leukemia ◽  
Children And Adolescents ◽  
Hepatitis B Surface Antigen ◽  
Hbv Vaccination ◽  
B Virus ◽  
Before And After ◽  
Positive Rate ◽  
Surface Antibody

Background: Vaccination for hepatitis B virus (HBV) after chemotherapy among pediatric patients with acute leukemia is still a debated issue. We investigated HBV immunity before and after chemotherapy and assessed immune response to re-vaccination after chemotherapy.  Methods:  We retrospectively analyzed data of children and adolescents aged <19 years requested for vaccination after chemotherapy for acute leukemia to evaluate hepatitis B surface antibody (HBsAb) status before and after chemotherapy and to identify factors related to HBsAb positivity after chemotherapy. Results:  Of 89 enrolled patients, 61 (68.5%) with acute leukemia were HBsAb positive before chemotherapy. Of these 61 patients, 48 (78.7%) seroconverted to HBsAb negative status after chemotherapy; there were 76 (85.4%) HBsAb negative patients after chemotherapy. HBsAb positive patients when compared to HBsAb negative patients after chemotherapy had a significantly higher HBsAb positive rate (100.0% vs. 63.2%, p=0.008) before chemotherapy. Following HBsAb testing after one dose of the HBV vaccination, 33 (43.4%) of the 76 HBsAb negative patients seroconverted to a HBsAb positive status. HBsAb positive patients after a single dose of HBV vaccination had a significantly higher HBsAb positive rate at the time of diagnosis compared to HBsAb negative patients (84.8% vs. 48.8%, p=0.001). Conclusions:  Based on these results, HBV re-vaccination after chemotherapy is recommended for all children and adolescents with acute leukemia. In addition, further investigation is required to improve the immunogenicity of HBV re-vaccination.   Keywords: Acute Leukemia; Chemotherapy; Hepatitis B vaccine; Hepatitis B virus; Child.

Ten-Year Persistence of Antibody to Hepatitis B Surface Antigen in Healthcare Workers Vaccinated Against Hepatitis B Virus, and Response to Booster Vaccination

2003 ◽  
Vol 24 (10) ◽  
pp. 773-776 ◽  
Author(s):  
Ricardo Durlach ◽  
Stella Laugas ◽  
Cristina B. Freuler ◽  
Viviana E. Rodríguez ◽  
Marta Costa
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Healthcare Workers ◽  
Hepatitis B Surface Antigen ◽  
Booster Vaccination ◽  
Hbv Vaccination ◽  
Kaplan Meier ◽  
B Virus ◽  
Antibody Levels

AbstractThis study estimated the number of HCWs with protective antibody levels 5 and 10 years after HBV vaccination. Kaplan-Meier probabilities of protective levels were 0.95 at 60 days after vaccination, 0.87 at 5 years, and 0.79 at 10 years. Those without protective levels displayed good response 7 and 30 days after a booster

Discrepant Hepatitis B Surface Antigen Results in Pregnant Women Screened to Identify Hepatitis B Virus Infection

2014 ◽  
Vol 165 (4) ◽  
pp. 773-778 ◽  
Author(s):  
Steven L. Veselsky ◽  
Tanja Y. Walker ◽  
Nancy Fenlon ◽  
Chong-Gee Teo ◽  
Trudy V. Murphy
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Pregnant Women ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Hepatitis B Virus Infection ◽  
B Virus

Sensitivity of hepatitis B virus DNA transcription-mediated amplification testing in hepatitis B surface antigen?positive blood donations

Transfusion ◽  
2006 ◽  
Vol 46 (12) ◽  
pp. 2047-2052 ◽  
Author(s):  
Françoise Bouchardeau ◽  
Annie Girault ◽  
Annie Razer ◽  
Annabelle Servant-Delmas ◽  
Mélanie Mercier ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Blood Donations ◽  
Dna Transcription ◽  
Hepatitis B Virus Dna ◽  
B Virus
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