Management of post-harvest Pectobacterium soft rot of cabbage (Brassica oleracea var capitata L.) by biocides and packing material

2012 ◽  
Vol 7 (28) ◽  
Author(s):  
K. A. Bhat
Keyword(s):  
Brassica Oleracea ◽  
Soft Rot ◽  
Packing Material ◽  
Post Harvest

scholarly journals Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽  
2016 ◽  
Vol 27 (3) ◽  
Author(s):  
Noor Istifadah ◽  
Muhamad Salman Umar ◽  
Sudarjat Sudarjat ◽  
Luciana Djaya
Keyword(s):  
Biological Control ◽  
Potato Tuber ◽  
Endophytic Bacteria ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Soft Rot Disease ◽  
Post Harvest ◽  
Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

Current Status of Post Harvest Soft Rot in Vegetables: A Review

2010 ◽  
Vol 9 (4) ◽  
pp. 200-208 ◽  
Author(s):  
K.A. Bhat ◽  
S.D. Masood ◽  
N.A. Bhat ◽  
M. Ashraf Bhat ◽  
S.M. Razvi ◽  
...  
Keyword(s):  
Soft Rot ◽  
Current Status ◽  
Post Harvest

scholarly journals Impact of Glucosinolate Content in Broccoli (Brassica oleracea (Italica Group)) on Growth of Pseudomonas marginalis, a Causal Agent of Bacterial Soft Rot

Plant Disease ◽  
2002 ◽  
Vol 86 (6) ◽  
pp. 629-632 ◽  
Author(s):  
Craig S. Charron ◽  
Carl E. Sams ◽  
Craig H. Canaday
Keyword(s):  
Brassica Oleracea ◽  
Degradation Products ◽  
Linear Regression Analysis ◽  
Soft Rot ◽  
Causal Agent ◽  
Glucosinolate Content ◽  
Bacterial Soft Rot ◽  
Vitro Assay ◽  
The Impact

Glucosinolate degradation products are known to suppress microbes. Brassica species produce glucosinolates. Previous investigations determined that susceptibility to bacterial soft rot of broccoli (Brassica oleracea (Italica group)) varied significantly by cultivar. To evaluate the impact of glucosinolates on Pseudomonas marginalis, a causal agent of bacterial soft rot, glucosinolates were measured in lyophilized florets from broccoli ‘Arcadia’, ‘Emperor’, ‘Green Comet’, ‘Green Valiant’, ‘Marathon’, ‘Packman’, ‘Premium Crop’, and ‘Shogun’. Total glucosinolate content was highest in ‘Shogun’ (29.8 μmol/g) and lowest in ‘Emperor’ (0.5 μmol/g). In an in vitro assay, simple linear regression analysis showed that 48% of differences in suppression of P. marginalis growth could be explained by differences in total glucosinolate content (P ≤ 0.01). Plant breeding efforts should include glucosinolate levels as a factor in selecting for disease resistance.

scholarly journals Impact of Pre- and Post–Harvest Applications of Natural Antimicrobial Products on Apple and Pear Soft Rot Disease

2016 ◽  
Vol 4 (3) ◽  
pp. 105-119
Author(s):  
Abdelradi T. Bakeer ◽  
Khaled Elbanna ◽  
Sameh A. Elnaggar
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
Antimicrobial Agents ◽  
Soft Rot ◽  
Natural Antimicrobial ◽  
Soft Rot Disease ◽  
Post Harvest ◽  
Rot Disease ◽  
Bacillus Strains

Three natural antibacterial compounds including bacteriocin like substance (BLS) produced from lactic acid bacteria (LAB), ethanolic extract of propolis (EEP), and nine plant extracts were evaluated against soft rot Bacillus strains. Testing in vivo these compounds were evaluated to control pear and apple soft rot disease. Among eight BLS tested, BLS of LAB2, LAB105 and LAB 107 exhibited the highest antibacterial activity as indicated by the formation of clear inhibition zone. Propolis extracts exhibited significant antibacterial activity against all tested soft rot Bacillus strains and it was noticed that the antibacterial activity was concentration dependent. Among nine plant extracts tested, extracts of Eucalyptus globulus and Psidium guajava exhibited the highest antibacterial activity. All tested antibacterial products significantly decreased apple and pear soft rot severity caused by Bacillus altitudinis compared to untreated control. The highest reduction percentage of soft rot severity was recorded for EEP followed by BLS from LAB and plant extracts tested, respectively. Combined pre-and post-harvest treatments of apple and pear with antimicrobial compounds proved to be more effective in reducing the soft rot severity and improved the physical and chemical properties of fruits during storage in both years of the study. The natural antimicrobial agents used in this study were promising compounds, since it seems to be more safe, economical and great potential for extending the shelf life and improve the quality of fruits. Therefore, the application of these compounds in the control of apple and pear soft rot could be advantageous for consumers, producers, and the environment.

scholarly journals Optimum O2 or CO2 Atmosphere for Storing Broccoli Florets at Various Temperatures

1996 ◽  
Vol 121 (1) ◽  
pp. 127-131 ◽  
Author(s):  
Hidemi Izumi ◽  
Alley E. Watada ◽  
Willard Douglas
Keyword(s):  
Oxygen Consumption ◽  
Brassica Oleracea ◽  
Ethylene Production ◽  
Soft Rot ◽  
Co2 Atmosphere

`Marathon' broccoli (Brassica oleracea L. var. italica) florets were stored in air, low O2(0.25%, 0.5%, and 1 %) or high CO2(3%,6%, and 10%) at 0, 5, and 10C. Oxygen consumption and CO2 production were reduced under low O2 or high CO2atmosphere, the reduction being greater at lower O2 and higher CO2 levels. No differences were found in ethylene production among the different atmospheres. Low O2 and high CO2 retained color of broccoli florets to about the same extent at 10C but had no effect at 0 and 5C. Development of soft rot and browning was suppressed by low O2 or high CO2, but offensive off-odor occurred in 0.25%02 at all temperatures and 0.5% O2 at 10C. These results indicate that the best O2 and CO2 levels seem to be 0.5% O2 and 10% CO2 at 0 and 5C, and 1% O2 and 10% CO2 at 10C.

scholarly journals Development and Application of a PCR-Based Molecular Marker for the Identification of High Temperature Tolerant Cabbage (Brassica oleracea var. capitata) Genotypes

Agronomy ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 116
Author(s):  
Hayoung Song ◽  
Myungjin Lee ◽  
Byung-Ho Hwang ◽  
Ching-Tack Han ◽  
Jong-In Park ◽  
...  
Keyword(s):  
High Temperature ◽  
Molecular Marker ◽  
Brassica Oleracea ◽  
Inbred Line ◽  
Genetic Basis ◽  
Soft Rot ◽  
Inbred Lines ◽  
Rna Seq ◽  
F2 Population ◽  
Pcr Based Molecular Marker

Global warming accelerates the development of high temperature (HT)- and high humidity (HH)-tolerant varieties. This is further facilitated by the identification of HTHH-tolerant genes and the development of molecular markers based on these genes. To identify genes involved in HTHH tolerance in cabbage (Brassica oleracea var. capitata), we performed RNA-seq analysis of two inbred lines, BN1 (HTHH-tolerant) and BN2 (HTHH-susceptible), and selected trehalose 6- phosphate phosphatase I-2 (BoTPPI-2) as one of the HTHH-tolerant-associated genes. We also developed a segregating F2 population from a cross between BN1 and BN2. RNA-seq results showed that BoTPPI-2 transcript levels were high in the HTHH-tolerant inbred line BN1, but not detectable in the HTHH-susceptible inbred line BN2. The expression pattern of BoTPPI-2 was not related to the expression of heat shock-related genes. Soft rot resistance, used as an indicator of HTHH tolerance, was higher in BN1 than in BN2. F2 individuals similar to BN1 with respect to phenotype appeared to be HTHH-tolerant, whereas BN2-types were susceptible to HTHH. Analysis of the genomic DNA revealed the presence of a long terminal repeat (LTR; ca. 4.6 kb) in the ninth intron of the BoTPPI-2_BN2 allele, thereby suppressing its transcription and exhibiting HTHH phenotype. Except for the LTR insertion, the sequence of BoTPPI-2_BN2 was almost identical to that of BoTPPI-2_BN1. On the basis of the LTR and BoTPPI-2 sequences, we developed a molecular marker to identify HTHH-tolerant genotypes and validated its efficiency using F2 individuals, inbred lines, and cultivars from diverse sources. The marker explained the genetic basis of HTHH tolerance in at least 80%, but not 100%, of the cabbage genotypes. Thus, additional markers associated with HTHH tolerance are needed for perfect selection.

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