scholarly journals In vitro cultivation of Pleurotus ostreatus and Lentinula edodes in lignocellulosic residues from Amazon

2013 ◽  
Vol 12 (46) ◽  
pp. 6526-6531
Author(s):  
Sales-Campos Ceci ◽  
A. Pires Diego ◽  
R. L. Barbosa Samira ◽  
Liege S. Abreu Raimunda ◽  
C. N. Meire
HortScience ◽  
1992 ◽  
Vol 27 (10) ◽  
pp. 1131-1133 ◽  
Author(s):  
J.J. Worrall ◽  
C.S. Yang

A mixture of apple pomace and sawdust was tested as a substrate for production of shiitake [Lentinula edodes (Berk.) Pegler] and oyster mushroom [Pleurotus ostreatus (Jacq. ex Fr.) Kummer and P. sajor-caju (Fr.) Sing.] on synthetic logs. MyCelia grew faster and more densely in logs containing apple pomace than in sawdust alone. Five shiitake isolates and two Pleurotus spp. produced higher fresh weights on a mixture of equal parts (by weight) of apple pomace and sawdust than on either substrate alone. An alternative substrate based on sawdust, millet (Panicum miliaceum L.), and wheat (Triticum aestivum L.), bran gave almost identical overall yield as pomace-sawdust medium, but there was a significant differential effect of the substrates on yield of the two tested shiitake isolates. Analyses and experiments in vitro suggested that optimal N levels provided by apple pomace account in part for its effectiveness.


Author(s):  
Alexandru Valentin ZĂGREAN ◽  
Gabriela NEAŢĂ ◽  
Bogdan STĂNCIULESCU

The mycelial growth rate of eight experimental strains - Pleurotus eryngii (4) and Lentinula edodes (4) - was studied on PDA medium at different incubation temperatures: 18°, 24° and 30°C.The aim of the investigation was to determine the optimal temperature for in vitro cultivation of each of the eight strains under study. All the experimental soushes reached their optimum growth at 24-30°C. P.eryngii strains showed better growth rates compared to those of L.edodes. The fastest strains were Pery-G (4,83 mm day-1/30oC), Pery-K (4,14 mm day-1/30oC) for P.eryngii and Led-S (3,35 mm day-1/30oC), LeM-51 (3,05 mm day-1/30oC) for L.edodes, respectively.Statistical interpretation shows that our experimental strains of both specia obtained very significant positive results at 30oC. 


Author(s):  
Samuel de Abreu LOPES ◽  
Thainá Louzada dos SANTOS ◽  
Sael Elias SÁNCHEZ ◽  
Ricardo Luiz Louro BERBARA ◽  
Andrés Calderín GARCIA

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1812
Author(s):  
Juncai Tu ◽  
Margaret Anne Brennan ◽  
Gang Wu ◽  
Weidong Bai ◽  
Ping Cheng ◽  
...  

Sorghum biscuits were enriched with mushroom powders (Lentinula edodes, Auricularia auricula and Tremella fuciformis) at 5%, 10% and 15% substitution levels. An in vitro gastrointestinal digestion was used to evaluate the effect of this enrichment on the phenolic content and soluble peptide content as well as antioxidant activities of the gastric or intestinal supernatants (bio-accessible fractions), and the remaining portions of phenolic compounds, antioxidants and β-glucan in the undigested residue (non-digestible fraction). The phenolic content of the gastric and intestinal supernatants obtained from digested mushroom-enriched biscuits was found to be higher than that of control biscuit, and the phenolic content was positively correlated to the antioxidant activities in each fraction (p < 0.001). L. edodes and T. fuciformis enrichment increased the soluble protein content (small peptide) of sorghum biscuits after in vitro digestion. All mushroom enrichment increased the total phenolic content and β-glucan content of the undigested residue and they were positively correlated (p < 0.001). The insoluble dietary fibre of biscuits was positively correlated with β-glucan content (p < 0.001) of undigested residue. These findings suggested that enriching food with mushroom derived dietary fibre increases the bioavailability of the non-digestible β-glucan and phenolic compounds.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.


2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Diane G. Edmondson ◽  
Steven J. Norris

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