Morphological and molecular identification of pathogenic fungal of post-harvest tomato fruit during storage

2012 ◽  
Vol 6 (22) ◽  
Author(s):  
Guofang Xie
Keyword(s):  
Molecular Identification ◽  
Tomato Fruit ◽  
Post Harvest

scholarly journals Alcobaça allele and genotypic backgrounds affect yield and fruit shelf life of tomato hybrids

2003 ◽  
Vol 60 (2) ◽  
pp. 269-275 ◽  
Author(s):  
Túlio José Mendes Dias ◽  
Wilson Roberto Maluf ◽  
Marcos Ventura Faria ◽  
Joelson André de Freitas ◽  
Luiz Antonio Augusto Gomes ◽  
...  
Keyword(s):  
Shelf Life ◽  
Fruit Quality ◽  
Tomato Fruit ◽  
Total Yield ◽  
Fruit Shape ◽  
Quality Traits ◽  
Average Mass ◽  
Post Harvest ◽  
Fruit Quality Traits ◽  
Commercial Yield

Post-harvest shelf life of tomato fruit may be increased by deploying mutant alleles which affect the natural ripening process and/or by a favorable genotypic background. Among the several ripening mutant genes, alcobaça (alc) has proved to be highly efficient in increasing shelf life of commercial tomato fruits, especially in heterozygosis, a state at which no limiting deleterious effects upon fruit color occur. The effects of heterozygosity in the alcobaça locus (alc+/alc) on yield and fruit quality traits of tomato hybrids with three genotypic backgrounds. We evaluated three pairs of hybrids obtained from crosses between the near-isogenic pollen source lines Flora-Dade (alc+/alc+) and TOM-559 (alc/alc), and three maternal lines (Stevens, NC-8276 and Piedmont). The six treatments were factorial combinations of two different status in the alc locus (alc+/alc and alc+/alc+) versus three different genotypic backgrounds (maternal lines). Fruits were harvested at the breaker stage of maturation and stored in shelves at 21ºC for 14 days. Yield and fruit quality traits were then evaluated. Regardless of the background, the alc allele in heterozygosis (alc+/alc) did not interfere with the total yield, commercial yield, average mass per fruit, average mass per commercial fruit, fruit shape, or with fruit peduncular scar diameter. The alc+/alc genotype reduced the rate of firmness loss and delayed evolution of the red color of the fruit, thus contributing to an increase of the post-harvest shelf life for all three genotypic backgrounds.

scholarly journals Isolatation and Identification of the Fungi Causing Tomato Fruit Rot Disease in the Vicinity of Tandojam, Sindh

2020 ◽  
Author(s):  
S. Nizamani ◽  
A. A. Khaskheli ◽  
A. M. Jiskani ◽  
S. A. Khaskheli ◽  
A. J. Khaskheli ◽  
...  
Keyword(s):  
Tomato Fruit ◽  
Aerial Mycelium ◽  
Alternaria Solani ◽  
Economic Loss ◽  
Geotrichum Candidum ◽  
Fruit Rot ◽  
Post Harvest ◽  
Standard Procedures ◽  
Fruit Samples ◽  
Rot Disease

Background: The post-harvest tomato fruit rot disease is common threat to the tomato fruit, causing huge economic loss as revealed by (GOP, 2018). The present study was conducted for isolatation and identification of causative agent of tomato fruit rot in order to formulate the proper management stretegies. Methods: Study was conducted in three phases. Phase one included collection of tomato fruit samples from vicinity of Tandojam. In phase two pathogens were isolated from the samples at laboratory, while in the phase three pathogens were identified using standard procedures. Result: The experimental results indicated Alternaria solani as the main cause of post-harvest tomato fruit rot. The symptoms observed were presence of brown to black rot lesions on tomato fruits with distinct rings ranging from small pin-heads to whole surface of fruit. A total of six different fungi viz., Alternaria alternata, Aspergillus niger, Alternaria solani, Geotrichum candidum, Fusarium oxysporum and Rhizopus stolonifer were found to be associated with post harvest tomato rot. Significantly higher infection was recorded for A. solani (53.667%) followed by A. niger (16.333%) and G. candidum (13.00%). The lowest infection percentage was observed for F. oxysporum (2.333%), followed by A. alternata (4.00%) and R. stolonifer (9.00%). A. solani produced aerial mycelium with yellowish to reddish diffusible pigments. A. niger cultures were typically black and colonies were initially whitish to yellow and later became brown to black in colour. G. candidum produced white and nonaerial colonies. F. oxysporum produced circular, aerial mycelium initially white, later changed to light pink. R. stolonifer produced whitish to grey fuzzy colonies.

Morphological and molecular identification of the causal agents of post-harvest diseases in avocado fruit, and potential biocontrol with Meyerozyma caribbica

2020 ◽  
pp. 1-20
Author(s):  
Maricarmen Iñiguez-Moreno ◽  
Katia Nayely González-Gutiérrez ◽  
Juan Arturo Ragazzo-Sánchez ◽  
José Alberto Narváez-Zapata ◽  
Teresa Sandoval-Contreras ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Post Harvest ◽  
Avocado Fruit

scholarly journals Effect of Elevated Temperature on Tomato Post-Harvest Properties

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2359
Author(s):  
Vera Thole ◽  
Philippe Vain ◽  
Cathie Martin
Keyword(s):  
Climate Change ◽  
Elevated Temperature ◽  
Shelf Life ◽  
Fruit Quality ◽  
High Field ◽  
Storage Temperature ◽  
Tomato Fruit ◽  
Field Performance ◽  
Fleshy Fruit ◽  
Post Harvest

The fleshy fruit of tomato (Solanum lycopersicum) is a commodity used worldwide as a fresh or processed product. Like many crops, tomato plants and harvested fruits are susceptible to the onset of climate change. Temperature plays a key role in tomato fruit production and ripening, including softening, development of fruit colour, flavour and aroma. The combination of climate change and the drive to reduce carbon emission and energy consumption is likely to affect tomato post-harvest storage conditions. In this study, we investigated the effect of an elevated storage temperature on tomato shelf life and fungal susceptibility. A collection of 41 genotypes with low and high field performance at elevated temperature, including different growth, fruit and market types, was used to assess post-harvest performances. A temperature increase from 18–20 °C to 26 °C reduced average shelf life of fruit by 4 days ± 1 day and increased fungal susceptibility by 11% ± 5% across all genotypes. We identified tomato varieties that exhibit both favourable post-harvest fruit quality and high field performance at elevated temperature. This work contributes to efforts to enhance crop resilience by selecting for thermotolerance combined with traits suitable to maintain and improve fruit quality, shelf life and pathogen susceptibility under changing climate conditions.

scholarly journals Post-harvest Management of Alternaria Induced Rot in Tomato Fruits With Essential Oil of Zanthoxylum armatum DC

2021 ◽  
Vol 5 ◽  
Author(s):  
Shummu Slathia ◽  
Yash Pal Sharma ◽  
Haroon Rashid Hakla ◽  
Mohammad Urfan ◽  
Narendra Singh Yadav ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tomato Fruit ◽  
Membrane Damage ◽  
Radical Scavenging ◽  
Fruit Rot ◽  
Gas Chromatography Mass Spectrometry ◽  
Harvest Management ◽  
Tomato Fruits ◽  
Post Harvest ◽  
Zanthoxylum Armatum

Alternaria fruit rot is a major disease caused by Alternaria alternata (Fr.) Keissl., a prolific fungal pathogen. Among post-harvest diseases of tomato, fruit rot induced by A. alternata is the most damaging. Antifungal agents are widely used to control post-harvest management of tomato fruits. However, negative impacts of fungicidal residues in edible fruits and vegetables on human health cannot be over ruled. Eco-friendly ways of controlling Alternaria rot in tomato fruits offer a novel way of tomato rot management. The current study proposes an alternate method in controlling tomato fruit rots through Zanthoxylum armatum DC essential oil (EO) application. Gas chromatography-mass spectrometry profiling showed eucalyptol and sabinene as major components of Z. armatum EO. Furthermore, EO applied (0.5–4.5 μl/ml) showed significant inhibition of A. alternata growth (p > 0.05) at 4.5 μl concentration tested. Lipid peroxidation assays revealed significant reduction in membrane damage in tomato fruits treated by EO compared to alone inoculated fruits with A. alternata. Elevated activities of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase coupled with enhanced antioxidants such as ascorbic acid, glutathione, proline, and total phenols in EO-treated fruits may be linked with better fruit rot management than control fruits inoculated with A. alternata-induced rot alone. Mycelia and spore production was dramatically reduced in EO applied tomato fruits over A. alternata alone in tomato fruits (p > 0.05). Interestingly, free radical scavenging activities of EO applied tomato fruits showed significant improvement compared to only pathogen-inoculated tomato fruits. Findings propose practical utility of Z. armatum EO as a plant-based antifungal for post-harvest management of Alternaria rot in tomato fruits.

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