scholarly journals A monoclonal antibody-based antigen-capture enzyme-linked immunosorbent assay (ELISA) for the detection of bluetongue virus

2013 ◽  
Vol 7 (41) ◽  
pp. 4892-4895 ◽  
Author(s):  
Yin Hui qiong ◽  
Yang Shu ◽  
Li Wen chao ◽  
Liu Jun ◽  
Wang Rui ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Bluetongue Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Capture

scholarly journals Antigen Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Reston Ebola Virus Nucleoprotein

2003 ◽  
Vol 10 (4) ◽  
pp. 552-557 ◽  
Author(s):  
Tetsuro Ikegami ◽  
Masahiro Niikura ◽  
Masayuki Saijo ◽  
Mary E. Miranda ◽  
Alan B. Calaor ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Amino Acid ◽  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Ebola Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Amino Acid Residues ◽  
Specific Detection ◽  
Antigen Capture ◽  
Cynomolgus Macaques

ABSTRACT Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.

scholarly journals Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay

1999 ◽  
Vol 6 (1) ◽  
pp. 130-132 ◽  
Author(s):  
S. L. Schoenthaler ◽  
S. Kapil
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Close Correlation ◽  
Antigen Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Coronavirus ◽  
Hemagglutination Test ◽  
Antigen Capture ◽  
Fecal Suspension

ABSTRACT We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 104 bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.

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