Antigen Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Reston Ebola Virus Nucleoprotein

ABSTRACT Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.

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Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus

Epidemiology and Infection ◽

10.1017/s0950268803008264 ◽

2003 ◽

Vol 130 (3) ◽

pp. 533-539 ◽

Cited By ~ 15

Author(s):

T. IKEGAMI ◽

M. SAIJO ◽

M. NIIKURA ◽

M. E. MIRANDA ◽

A. B. CALAOR ◽

...

Keyword(s):

Amino Acid ◽

Immunosorbent Assay ◽

Specific Antibody ◽

Immunoglobulin G ◽

Ebola Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Cynomolgus Macaques

We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360–739), and RΔ6 (aa 451–551) and/or RΔ8 (aa 631–739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.

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Identification of a Secreted Cholesterol-Dependent Cytolysin (Mitilysin) from Streptococcus mitis

Journal of Bacteriology ◽

10.1128/jb.01092-06 ◽

2006 ◽

Vol 189 (2) ◽

pp. 627-632 ◽

Cited By ~ 17

Author(s):

Johanna Jefferies ◽

Leena Nieminen ◽

Lea-Ann Kirkham ◽

Calum Johnston ◽

Andrew Smith ◽

...

Keyword(s):

Monoclonal Antibody ◽

Streptococcus Pneumoniae ◽

Amino Acid ◽

Immunosorbent Assay ◽

Pneumococcal Disease ◽

Neutralization Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Amino Acid Substitutions ◽

Streptococcus Mitis ◽

Hemolytic Toxin

ABSTRACT We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mitilysin extracellularly. Certain alleles of mitilysin are not recognized by a monoclonal antibody raised to the related toxin pneumolysin. Based on enzyme-linked immunosorbent assay and neutralization assay results, one isolate of S. mitis may produce a further hemolytic toxin in addition to mitilysin. As genetic exchange is known to occur between S. mitis and Streptococcus pneumoniae, this finding may have implications for the development of vaccines or therapies for pneumococcal disease that are based on pneumolysin.

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Rapid Detection of Salmonella enterica Serovar Choleraesuis in Blood Cultures by a Dot Blot Enzyme-Linked Immunosorbent Assay

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.7.6.977-979.2000 ◽

2000 ◽

Vol 7 (6) ◽

pp. 977-979 ◽

Cited By ~ 8

Author(s):

Kritsana Janyapoon ◽

Sunee Korbsrisate ◽

Hatairat Thamapa ◽

Sittichai Thongmin ◽

Suwattana Kanjanahareutai ◽

...

Keyword(s):

Monoclonal Antibody ◽

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Salmonella Enterica ◽

Rapid Detection ◽

Direct Detection ◽

Blood Cultures ◽

Enzyme Linked Immunosorbent Assay ◽

Biochemical Method ◽

Dot Blot

ABSTRACT A dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella entericaserovar Choleraesuis in blood cultures. This system was applied to the identification of serovar Choleraesuis, and the results were compared with those obtained by a conventional biochemical method. It was revealed that all 12 samples identified to be infected with serovar Choleraesuis were positive on testing by the ELISA. In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%.

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