Role of fluorescence in situ hybridization in the diagnosis of urothelial cancer in asymptomatic hematuria patients

2011 ◽  
Vol 6 (26) ◽  
Author(s):  
Peng Wang
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Urothelial Cancer

Refining the Breakpoints of Three New Translocations Identified in Myelodysplastic Syndromes

2015 ◽  
Vol 135 (2) ◽  
pp. 94-100
Author(s):  
Dolors Costa ◽  
Concha Muñoz ◽  
Ana Carrió ◽  
Amparo Arias ◽  
Cándida Gómez ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Myelodysplastic Syndromes ◽  
Tp53 Gene ◽  
Neoplastic Process ◽  
Conventional Cytogenetics

Recurrent translocations are uncommon in myelodysplastic syndromes (MDS). Three new recurrent translocations, namely der(12)t(3;12)(q13;p13), t(11;13;22)(q13;q14;q12) and der(17)t(13;17)(q21;p13), identified by conventional cytogenetics (CC) in 4 MDS patients, were further characterized using a panel of commercial and homemade fluorescence in situ hybridization (FISH) probes. The goal of this study was to determine the precise breakpoints and to identify genes that could be related with the neoplastic process. Half of the breakpoints (4/8) were precisely identified and in the remaining half they were narrowed to a region ranging from 14 to 926 kb. All the studied breakpoints had interstitial or terminal deletions ranging from 536 kb to 89 Mb, and only those 7 Mb were detected by CC. The genes located in or around the breakpoints described in our study have not been previously related to MDS. The deleted regions include the ETV6 and RB1 genes, among others, and exclude the TP53 gene. FISH studies were useful to refine the breakpoints of the translocations, but further studies are needed to determine the role of the involved genes in the neoplastic process.

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