Effects of Artificial Saliva Contamination on the Bond Strength of Three Dentin Adhesives to Dentin of Primary Teeth

AbstractBackgroundloss of hybrid layer integrity compromises the resin-dentin bond stability. Subsequent release and activation of matrix metalloproteinase enzymes during dental restorative procedures or caries development are contributing factors to dentin-adhesive bond failure.AimTo investigate the effect of pre-treatment with different MMP inhibitors on the shear bond strength (SBS) of an etch-and-rinse adhesive (Adper Single Bond 2) to primary teeth dentin after six months of aging.MethodsSixty extracted anterior primary teeth, due to orthodontic reasons, were selected. A dentin block (6.0 mm × 6.0 mm × 2.0 mm) was obtained from each tooth. All the dentin blocks (n = 60) were pretreated for 60 s with either 0.01 M phosphate buffered saline (PBS, pH 7.2) in the control group, 2%: Chlorhexidine (CHX) solution, 2% Doxycycline (DO)solution and EDTA 17% after etching and before applying etch-and-rinse adhesive system (Adper Single Bond 2). Then composite was applied in clear teflon cylinders. The specimens were stored in artificial saliva for 6 months at 37°C and then the SBS values were determined with a universal testing machine. Representative samples were selected for visualization of the failure mode under stereomicroscope and SEM.ResultsNo statistically significant difference observed between the SBS of the EDTA group, DO group and the control group (P value>0.05). CHX showed significantly higher SBS values compared to the other groups of the study after six months(P value=0.011).ConclusionChlorhexidine significantly preserved the SBS of composite resin to deciduous dentin using etch and rinse adhesive Adper Single Bond 2 after 6 months of aging in artificial saliva.

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Artificial Saliva Contamination Effects on Bond Strength of Self-etching Primers

The Angle Orthodontist ◽

10.2319/0003-3219(2008)078[0716:asceob]2.0.co;2 ◽

2008 ◽

Vol 78 (4) ◽

pp. 716-721 ◽

Cited By ~ 8

Author(s):

Ekaterini Paschos ◽

Jean-Oliver Westphal ◽

Nicoleta Ilie ◽

Karin Christine Huth ◽

Reinhard Hickel ◽

...

Keyword(s):

Bond Strength ◽

Artificial Saliva ◽

Saliva Contamination ◽

Contamination Effects

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Influence of Zinc-Oxide Eugenol, Formocresol, and Ferric Sulfate on Bond Stength of Dentin Adhesives to Primary Teeth

The Journal of Contemporary Dental Practice ◽

10.5005/jcdp-6-3-14 ◽

2005 ◽

Vol 6 (3) ◽

pp. 14-21 ◽

Cited By ~ 5

Author(s):

Fouad Saad Sakanam

Keyword(s):

Zinc Oxide ◽

Bond Strength ◽

Primary Teeth ◽

Testing Machine ◽

Adhesive System ◽

Ferric Sulfate ◽

Adhesive Resin ◽

Zinc Oxide Eugenol ◽

Dentin Adhesives

Abstract This study evaluated in vitro the influence of a temporary filling {zinc oxide-eugenol (ZOE)} and two pulpotomy agents {formocresol (FC) and ferric sulfate (FS)} on shear bond strength (SBS) of two dentin adhesives to the dentin of primary molars. A total of 80 dentin surfaces were prepared and randomly allocated into 10 groups of 8 specimens each. Groups were subjected to different treatments, which included covering with a paste of ZOE mixed at different powder:liquid (P:L) ratios, placement on a gauze soaked in FC or FS, or they received no pretreatment and served as a control. XRVTM Herculite® composite cylinders were bonded to dentin surfaces using Prime and Bond® NT adhesive resin or Opti Bond Solo Plus adhesive resin. SBSs were determined using the lnstron® testing machine running at a crosshead speed of 0.5 mm/min. The use of ZOE mixed at the lower P:L ratio of 10g:2g significantly decreased the values of SBS of the two adhesives. The use of two pulpotomy agents (FC and FS) significantly decreased the SBS of the two adhesives. The bond strength to dentin of primary teeth was influenced by the pulpotomy agents used and the ZOE P:L ratio but not by the adhesive system used. Citation Salama FS. Influence of Zinc-Oxide Eugenol, Formocresol, and Ferric Sulfate on Bond Strength of Dentin Adhesives to Primary Teeth. J Contemp Dent Pract 2005 August;(6)3:014-021.

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Effect of Artificial Saliva Contamination on Bond Strength to Pulp Chamber Dentin

European Journal of Dentistry ◽

10.1055/s-0039-1697360 ◽

2008 ◽

Vol 02 (02) ◽

pp. 086-090 ◽

Cited By ~ 7

Author(s):

Hale Ară ◽

Nazmiye Dönmez ◽

Sema Belli

Keyword(s):

Bond Strength ◽

Artificial Saliva ◽

In Vitro Study ◽

Adhesive System ◽

Control Group ◽

Pulp Chamber ◽

Significant Difference ◽

Group 2 ◽

Saliva Contamination

ABSTRACTObjectives: The purpose of this in vitro study was to evaluate the effect of artificial saliva contamination on microtensile bond strength to pulp chamber dentin.Methods: Clearfil SE Bond (SEB) (Kuraray, JAPAN) adhesive system and Clearfil Photo Posterior (CPP) (Kuraray, JAPAN) composite resin were used. Twenty extracted caries-free human molar teeth were randomly distributed into four groups. Apart from a control group without contamination (Group 1), primed dentin surfaces were contaminated with artificial saliva (10 s), rinsed, dried, reprimed and bonded (Group 2), coated with adhesive, contaminated with artificial saliva, rinsed, dried, bonding procedures were repeated (Group 3), coated with adhesive, light cured, contaminated with saliva, rinsed, dried, treated with SE primer (SEP) and SEB (Group 4).After 24 hrs, the teeth were prepared for microtensile bond testing and tensile bond strength was measured (1mm/min). The data was calculated as MPa and analyzed using one-way ANOVA and Duncan test (P<.05).Results: The results indicated that Group 2 showed lowest bond strength when compared to the others (P<.05). No statistically significant difference was found between Groups 3 and 4 (P>.05).Conclusions: It was concluded that contamination during priming procedure has a negative effect on bond strength (P<.05). Although contamination of the uncured adhesive was not critical in this study (P>.05) any kind of contamination of the bonding area should, in principle, be avoided. (Eur J Dent 2008;2:86-90)

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Shear bond strength of metallic brackets: influence of saliva contamination

Journal of Applied Oral Science ◽

10.1590/s1678-77572009000300011 ◽

2009 ◽

Vol 17 (3) ◽

pp. 190-194 ◽

Cited By ~ 9

Author(s):

Luciana Borges Retamoso ◽

Fabrício Mezzomo Collares ◽

Eduardo Silveira Ferreira ◽

Susana Maria Werner Samuel

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Saliva Contamination

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Effect of saliva contamination and re-etching time on the shear bond strength of a pit and fissure sealant

Journal of Applied Oral Science ◽

10.1590/s1678-77572004000300007 ◽

2004 ◽

Vol 12 (3) ◽

pp. 200-204 ◽

Cited By ~ 2

Author(s):

Gisele Maria Correr ◽

Angela S. Caldo-Teixeira ◽

Roberta Caroline Bruschi Alonso ◽

Regina Maria Puppin-Rontani ◽

Mário Alexandre Coelho Sinhoreti ◽

...

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Etching Time ◽

Control Group ◽

Distilled Water ◽

Third Molars ◽

Fissure Sealant ◽

Significant Difference ◽

Pit And Fissure Sealant ◽

Saliva Contamination

The aim of this study was to evaluate the effect of saliva contamination (SCT) and re-etching time (RET) on the shear bond strength (SBS) of the Fluroshield sealant. Forty-five extracted third molars were sectioned and flattened until reach an enamel surface area. Then, all samples were etched for 30 sec with 35% phosphoric acid and then they were distributed into 9 groups (n=10) according to SCT and RET (seconds), respectively: G1- control (no SCT and no RET); G2- 30s and 0s; G3- 60s and 0s; G4-30s and 2s; G5- 30s and 5s; G6- 30s and 15s; G7-60s and 2s; G8- 60s and 5s; G9- 60s and 15s. The sealant was applied according to the manufacturer's instructions. The samples were stored in distilled water at 37ºC for 72h and subjected to the SBS test. The results indicated that there was no statistically significant difference between the groups (p>0.05). However, it could be noticed that: 1- the longer the SCT, the lower the SBS values; 2 - the longer the RET, the higher the SBS values. It could be concluded that there was a tendency to the shortest SCT (30s) associated to the longest RET (5 and 15s) to reach similar SBS values for the control group.

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Adhesion procedure for CAD/CAM resin crown bonding: Reduction of bond strengths due to artificial saliva contamination

Journal of Prosthodontic Research ◽

10.1016/j.jpor.2017.08.006 ◽

2018 ◽

Vol 62 (2) ◽

pp. 177-183 ◽

Cited By ~ 11

Author(s):

Asuka Kawaguchi-Uemura ◽

Atsushi Mine ◽

Mariko Matsumoto ◽

Yuko Tajiri ◽

Mami Higashi ◽

...

Keyword(s):

Artificial Saliva ◽

Cad Cam ◽

Bond Strengths ◽

Saliva Contamination

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Effect of saliva contamination on bond strength witha hydrophilic composite resin

Dental Press Journal of Orthodontics ◽

10.1590/s2176-94512013000100015 ◽

2013 ◽

Vol 18 (1) ◽

pp. 63-68 ◽

Cited By ~ 5

Author(s):

Mauren Bitencourt Deprá ◽

Josiane Xavier de Almeida ◽

Taís de Morais Alves da Cunha ◽

Luis Filipe Siu Lon ◽

Luciana Borges Retamoso ◽

...

Keyword(s):

Bond Strength ◽

Composite Resin ◽

Color Change ◽

Resin Composite ◽

Bonding Material ◽

Adhesive Remnant Index ◽

Composite Methods ◽

Standard Deviations ◽

Saliva Contamination

OBJECTIVE: To evaluate the influence of saliva contamination on the bond strength of metallic brackets bonded to enamel with hydrophilic resin composite. METHODS: Eighty premolars were randomly divided into 4 groups (n = 20) according to bonding material and contamination: G1) bonded with Transbond XT with no saliva contamination, G2) bonded with Transbond XT with saliva contamination, G3) bonded with Transbond Plus Color Change with no saliva contamination and G4) bonded with Transbond Plus Color Change with saliva contamination. The results were statistically analyzed (ANOVA/Tukey). RESULTS: The means and standard deviations (MPa) were: G1)10.15 ± 3.75; G2) 6.8 ± 2.54; G3) 9.3 ± 3.36; G4) 8.3 ± 2.95. The adhesive remnant index (ARI) ranged between 0 and 1 in G1 and G4. In G2 there was a prevalence of score 0 and similar ARI distribution in G3. CONCLUSION: Saliva contamination reduced bond strength when Transbond XT hydrophobic resin composite was used. However, the hydrophilic resin Transbond Plus Color Change was not affected by the contamination.

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