Performance Evaluation of a Portable Laser-Induced Fluorescence Spectroscopy System for the Assessment of the Humification Degree of the Soil Organic Matter

The Integrated Crop-Livestock-Forest Systems (CLF) have been able to capture and store the carbon (C) in the form of Soil Organic Matter (SOM), in different regions in Brazil, thereby contributing to mitigate agricultural greenhouse gases emission. This is an eligible practice in Low Carbon Emission Agriculture Plan in Brazil, and currently has around 15 million hectares under use, a very positive and important trend in soil land use in Brazil. SOM is considered a relevant indicator of soil quality due to its direct relationship with biological, chemical, and physical properties, allowing it to evaluate the impacts of agricultural management. Laser-based spectroscopies as Laser-Induced Fluorescence Spectroscopy (LIFS) and Laser-Induced Breakdown Spectroscopy (LIBS) have become promising tools in the evaluation of the SOM in agricultural soils. LIBS can measure soil C, and LIFS can infer about the chemical structure of SOM, mainly aromaticity. The standard protocol for measuring soil C changes involves soil sampling at the field and chemical sample preparation for laboratory analysis. Although this procedure produces precise results, it takes time, generates chemical residues, and the costs restrict its routine for large scale use in agricultural projects. Thus, there is a need to develop clean (green chemistry), rapid, precise, and cost-efficient methods for measuring soil C changes in the field. Also, information about the chemical structure of SOM usually is done through spectroscopic techniques, such as 13C NMR, EPR, and fluorescence of humic acid, which are not applied for large scale measurement and mapping. LIFS can be applied in whole soil and can be used to evaluate the aromaticity of SOM, and consequently, its chemical stability.&#160; The objectives of this study were to evaluatethe soil C stock and SOM Stability of some Brazilian soils under different integrated systems, such as,Crop-Livestock-Forest (CLF), Crop-Livestock (CL) and Livestock-Forest(LF). The results showed the combination of soil carbon accumulation, and an increase of SOM aromaticity for CLF, which can be promising for sustainable intensification in agriculture.Keywords: Sustainable Intensification; Soil Organic Matter; Carbon stock; Laser-Induced Fluorescence Spectroscopy; Integrated Crop-Livestock-Forest Systems

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Organic Matter Study of Whole Soil Samples Using Laser-Induced Fluorescence Spectroscopy

Soil Science Society of America Journal ◽

10.2136/sssaj2004.0270 ◽

2006 ◽

Vol 70 (1) ◽

pp. 57-63 ◽

Cited By ~ 71

Author(s):

Débora Marcondes Bastos Pereira Milori ◽

Helder Vinicius Avanço Galeti ◽

Ladislau Martin-Neto ◽

Jeferson Dieckow ◽

Martha González-Pérez ◽

...

Keyword(s):

Organic Matter ◽

Fluorescence Spectroscopy ◽

Laser Induced Fluorescence ◽

Soil Samples

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CO2-C losses and carbon quality of selected Maritime Antarctic soils

Antarctic Science ◽

10.1017/s0954102012000648 ◽

2012 ◽

Vol 25 (1) ◽

pp. 11-18 ◽

Cited By ~ 9

Author(s):

Juliana Vanir De Souza Carvalho ◽

Eduardo De Sá Mendonça ◽

Newton La Scala ◽

César Reis ◽

Efrain Lázaro Reis ◽

...

Keyword(s):

Organic Matter ◽

Soil Organic Matter ◽

Soil Temperature ◽

Soil Carbon ◽

Humic Substances ◽

Microbial Activity ◽

Chemical Characteristics ◽

Maritime Antarctic ◽

Antarctic Soils ◽

Humification Degree

AbstractPolar Regions are the most important soil carbon reservoirs on Earth. Monitoring soil carbon storage in a changing global climate context may indicate possible effects of climate change on terrestrial environments. In this regard, we need to understand the dynamics of soil organic matter in relation to its chemical characteristics. We evaluated the influence of chemical characteristics of humic substances on the process of soil organic matter mineralization in selected Maritime Antarctic soils. A laboratory assay was carried out with soils from five locations from King George Island. We determined the contents of total organic carbon, oxidizable carbon fractions of soil organic matter, and humic substances. Two in situ field experiments were carried out during two summers, in order to evaluate the CO2-C emissions in relation to soil temperature variations. The overall low amounts of soil organic matter in Maritime Antarctic soils have a low humification degree and reduced microbial activity. CO2-C emissions showed significant exponential relationship with temperature, suggesting a sharp increase in CO2-C emissions with a warming scenario, and Q10 values (the percentage increase in emission for a 10°C increase in soil temperature) were higher than values reported from elsewhere. The sensitivity of the CO2-C emission in relation to temperature was significantly correlated with the humification degree of soil organic matter and microbial activity for Antarctic soils.

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Novel estimation of the humification degree of soil organic matter by laser-induced breakdown spectroscopy

Spectrochimica Acta Part B Atomic Spectroscopy ◽

10.1016/j.sab.2014.06.016 ◽

2014 ◽

Vol 99 ◽

pp. 76-81 ◽

Cited By ~ 29

Author(s):

Edilene Cristina Ferreira ◽

Ednaldo José Ferreira ◽

Paulino Ribeiro Villas-Boas ◽

Giorgio Saverio Senesi ◽

Camila Miranda Carvalho ◽

...

Keyword(s):

Organic Matter ◽

Soil Organic Matter ◽

Laser Induced Breakdown Spectroscopy ◽

Breakdown Spectroscopy ◽

Laser Induced Breakdown ◽

Humification Degree

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An adapted sequential chemical fractionation coupled with UV and fluorescence spectroscopy for calcareous soil organic matter study after compost amendment

Microchemical Journal ◽

10.1016/j.microc.2015.08.007 ◽

2016 ◽

Vol 124 ◽

pp. 139-148 ◽

Cited By ~ 10

Author(s):

Olsen Rainness Mouloubou ◽

Pascale Prudent ◽

Stéphane Mounier ◽

Jean-Luc Boudenne ◽

Madi Guirema Abaker ◽

...

Keyword(s):

Organic Matter ◽

Soil Organic Matter ◽

Fluorescence Spectroscopy ◽

Calcareous Soil ◽

Chemical Fractionation ◽

Compost Amendment

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Laser-based spectroscopic methods to evaluate the humification degree of soil organic matter in whole soils: a review

Journal of Soils and Sediments ◽

10.1007/s11368-016-1539-6 ◽

2016 ◽

Vol 18 (4) ◽

pp. 1292-1302 ◽

Cited By ~ 11

Author(s):

Giorgio S. Senesi ◽

Ladislau Martin-Neto ◽

Paulino Ribeiro Villas-Boas ◽

Gustavo Nicolodelli ◽

Débora M. B. P. Milori

Keyword(s):

Organic Matter ◽

Soil Organic Matter ◽

Spectroscopic Methods ◽

Humification Degree

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Fluorescence spectroscopy: A sensitive tool for identifying land-use and climatic region effects on the characteristics of water-extractable soil organic matter

Ecological Indicators ◽

10.1016/j.ecolind.2020.107103 ◽

2021 ◽

Vol 121 ◽

pp. 107103

Author(s):

Oshri Rinot ◽

Mikhail Borisover ◽

Guy J. Levy ◽

Gil Eshel

Keyword(s):

Land Use ◽

Organic Matter ◽

Soil Organic Matter ◽

Fluorescence Spectroscopy ◽

Climatic Region ◽

Sensitive Tool ◽

Water Extractable ◽

Extractable Soil

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Change and spatial variability of soil organic matter humification after long-term tillage and olive mill wastewater application in arid regions

Soil Research ◽

10.1071/sr19113 ◽

2020 ◽

Vol 58 (4) ◽

pp. 388

Author(s):

Hadda Ben Mbarek ◽

Kamel Gargouri ◽

Chaker Mbadra ◽

Rayda Chaker ◽

Yousra Souidi ◽

...

Keyword(s):

Organic Matter ◽

Soil Organic Matter ◽

Management Practices ◽

Fluorescence Emission ◽

Olive Mill Wastewater ◽

Emission Area ◽

Humification Degree ◽

Uv Visible ◽

Olive Mill

The changes of soil organic matter (SOM) humification induced by long-term combination of tillage and olive mill wastewater (OMW) application compared to natural and cultivated soil have been little investigated. This study aimed to compare effects of no cultivation with natural vegetation soil (NC), tillage (CT1) for 80 years and combination of tillage with OMW application (CT2) for 20 years on SOM humification degree. Fluorescence spectroscopy and UV-visible ratios (E4/E6 and CHA/CFA) were used to study soil humic acids (HAs). The SOM and humification distribution was determined for the whole field area using the Inverse Distance Weighting method. Results showed that SOM content, fluorescence emission area and E4/E6 and CHA/CFA ratios were higher in NC. Tillage reduced SOM amount, molecular size, aromatic condensation and humification degree as shown by the strong correlation between fluorescence area and CHA/CFA ratio in CT1 conversely to E4/E6. Contradictory results between fluorescence emission area and E4/E6 ratio found in NC and CT1 indicated that E4/E6 ratio was not a reliable indicator of SOM humification degree. The SOM amount, CHA/CFA ratio and emission fluorescence area increased conversely to E4/E6 ratio in CT2. This revealed a greatly humified organic matter and aromatic structure condensation with tillage and OMW application. Spatial distribution showed a progressive increase of SOM and CHA/CFA from north-west to south-east linked to the positive relationship between CHA/CFA ratio and SOM amount independent of soil management practices. Soil amended with OMW provided a favourable environment for the development of HAs which improved soil quality. The UV-visible ratio CHA/CFA with fluorescence emission area can be used as parameters to investigate SOM humification degree.

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