Incorporating molecular data in fungal systematics: a guide for aspiring researchers

The Proclossiana eunomia (Esper, 1799) complex is currently composed of the several subspecies distributed throughout Palaearсtic region and North America. Despite the fact that some of the taxa have differences in wing pattern and body size, previous assumptions on taxonomy not supported by molecular data. Therefore, the identity of certain populations of this complex has remained unclear and the taxonomic status of several recently described taxa is debated. Here, we provide insights into systematics of some Palaearctic members of this group using molecular approach, based on the analysis of the barcoding fragment of the COI gene taking into account known morphological differences.

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New Record of a Genus and Species of Odd-Scaled Snake (Serpentes: Xenodermidae) from China

Russian Journal of Herpetology ◽

10.30906/1026-2296-2020-27-6-348-352 ◽

2020 ◽

Vol 27 (6) ◽

pp. 348-352

Author(s):

Cai De Bo ◽

Zening Chen ◽

Jun Gao ◽

Ding Li ◽

Rong Dai

Keyword(s):

New Record ◽

First Record ◽

Molecular Data ◽

Extent Of Occurrence ◽

Morphological And Molecular Data

Parafimbrios has been reported in Laos, Thailand and Vietnam. In this paper, based on morphological and molecular data, Parafimbrios lao was reported as the first record of a genus and species of odd-scaled snake in China. This record extends the extent of occurrence to 65,000 km2 and the area of occurrence to 20 km2.

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Analysis of the differences between Syntormon pallipes and S. pseudospicatus (Diptera: Dolichopodidae): morphological and molecular data

Zoosystematica Rossica ◽

10.31610/zsr/2019.28.2.305 ◽

2019 ◽

Vol 28 (2) ◽

pp. 305-316

Author(s):

M.A. Chursina ◽

I.Ya. Grichanov

Keyword(s):

Dna Analysis ◽

Distinct Species ◽

Molecular Data ◽

Wing Shape ◽

Morphological Data ◽

12S Rrna ◽

Separate Species ◽

Geometric Morphometric ◽

Geometric Morphometric Analysis ◽

Morphological And Molecular Data

The recent catalogues of the family Dolichopodidae considered Syntormon pallipes (Fabricius, 1794) and S. pseudospicatus Strobl, 1899 as separate species. In this study, we used three approaches to estimate the significance of differences between the two species: molecular analysis (COI and 12S rRNA sequences), analysis of leg colour characters and geometric morphometric analysis of wing shape. The morphological data confirmed the absence of significant differences between S. pallipes and S. pseudospicatus found in the DNA analysis. Significant differences in the wing shape of two species have not been revealed. Hence, according to our data, there is no reason to consider S. pseudospicatus as a distinct species.

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Receptor tyrosine kinase gene amplification is predictive of intraoperative seizures during glioma resection with functional mapping

Journal of Neurosurgery ◽

10.3171/2018.12.jns182700 ◽

2020 ◽

Vol 132 (4) ◽

pp. 1017-1023 ◽

Cited By ~ 2

Author(s):

Bryan D. Choi ◽

Daniel K. Lee ◽

Jimmy C. Yang ◽

Caroline M. Ayinon ◽

Christine K. Lee ◽

...

Keyword(s):

Tyrosine Kinases ◽

Univariate Analysis ◽

Tumor Resection ◽

Tumor Grade ◽

Molecular Data ◽

Genetic Alterations ◽

Functional Mapping ◽

Chi Square ◽

Kinase Gene ◽

Glioma Resection

OBJECTIVEIntraoperative seizures during craniotomy with functional mapping is a common complication that impedes optimal tumor resection and results in significant morbidity. The relationship between genetic mutations in gliomas and the incidence of intraoperative seizures has not been well characterized. Here, the authors performed a retrospective study of patients treated at their institution over the last 12 years to determine whether molecular data can be used to predict the incidence of this complication.METHODSThe authors queried their institutional database for patients with brain tumors who underwent resection with intraoperative functional mapping between 2005 and 2017. Basic clinicopathological characteristics, including the status of the following genes, were recorded: IDH1/2, PIK3CA, BRAF, KRAS, AKT1, EGFR, PDGFRA, MET, MGMT, and 1p/19q. Relationships between gene alterations and intraoperative seizures were evaluated using chi-square and two-sample t-test univariate analysis. When considering multiple predictive factors, a logistic multivariate approach was taken.RESULTSOverall, 416 patients met criteria for inclusion; of these patients, 98 (24%) experienced an intraoperative seizure. Patients with a history of preoperative seizure and those treated with antiepileptic drugs prior to surgery were less likely to have intraoperative seizures (history: OR 0.61 [95% CI 0.38–0.96], chi-square = 4.65, p = 0.03; AED load: OR 0.46 [95% CI 0.26–0.80], chi-square = 7.64, p = 0.01). In a univariate analysis of genetic markers, amplification of genes encoding receptor tyrosine kinases (RTKs) was specifically identified as a positive predictor of seizures (OR 5.47 [95% CI 1.22–24.47], chi-square = 5.98, p = 0.01). In multivariate analyses considering RTK status, AED use, and either 2007 WHO tumor grade or modern 2016 WHO tumor groups, the authors found that amplification of the RTK proto-oncogene, MET, was most predictive of intraoperative seizure (p < 0.05).CONCLUSIONSThis study describes a previously unreported association between genetic alterations in RTKs and the occurrence of intraoperative seizures during glioma resection with functional mapping. Future models estimating intraoperative seizure risk may be enhanced by inclusion of genetic criteria.

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