Antimicrobial Activity of Aqueous, Ethanolic Extracts of Nigella Sativa (Black Cumin) Against Pathogenic Bacteria Isolation from Digestive Tract in Poultry

2019 ◽  
Vol 13 (4) ◽  
pp. 618
Author(s):  
Hala Mohammed Majeed
Keyword(s):  
Antimicrobial Activity ◽  
Digestive Tract ◽  
Pathogenic Bacteria ◽  
Nigella Sativa ◽  
Black Cumin ◽  
Ethanolic Extracts

scholarly journals Interaksi Ekstrak Etanol Biji Nigella sativa Terhadap Bakteri Probiotik dan Patogen

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Ahmad Shobrun Jamil
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Solid Medium ◽  
Nigella Sativa ◽  
Health Effect ◽  
Antimicrobial Effect ◽  
Inhibitory Effect ◽  
Semi Solid ◽  
Nigella Sativa Seed ◽  
Lactobacillus Spp

Nigella sativa seed has a lot of beneficial health effect, such as immune-modulator, anti-histamine, anti-diabetic, anti-hypertension, anti-inflammatory, anti-microbes and antitumor. Numeral antimicrobial effect of Nigella sativa has been published, but only a few number of the information of the antimicrobial activity to the probiotic bacteria such as from the genus of Lactobacillus spp. However this research focused on the selectivity of the antimicrobial activity of N. sativa extract to the probiotic and pathogenic bacteria. This research begins with the extraction of N. sativa seed powder by ethanol 80% 1:3 (w/v). With rotary evaporator the macerates extracted till gained semi solid extract. In the other side probiotic and pathogenic bacteria broth cultures were prepared. Extract resoluted with concentration 5%; 2,5%; 1,25% and 0,625%. Into the extract were submerged some disk paper with 6 mm diameter. After that the disk put on to the solid medium were had cultured by bacteria then incubated overnight. The diameters of the clearing zone that seemed then measured. The result showing that the inhibitory zone occurred at 0,625% concentration. After general analysis there is no significant different inhibitory effect of the extract to the tested bacteria, but from the statistical analysis known that probiotik bacteria seemed more sensitive than pathogenic bacteria were tested.

scholarly journals ANTIBACTERIAL EFFECTS OF SEVEN ESSENTIAL PLANT OILS ON FISH PATHOGENS

2020 ◽  
Vol 46 (3) ◽  
pp. 1-9
Author(s):  
Esin BABA ◽  
Keyword(s):  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Nigella Sativa ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Ocimum Sanctum ◽  
Yersinia Ruckeri ◽  
Fish Pathogens ◽  
Black Cumin

The use of natural products which have the least harmful effects on the environment has recently been taken as a novel approach against fish diseases. References on in vitro studies have demonstrated antibacterial activity of essential oils (EOs) against certain fish pathogens. The aim of this study was to evaluate the antibacterial effect of some plant essential oils against fish pathogenic bacteria in vitro conditions. Seven plant EOs: lavender (Lavandula angustifolia), clove (Eugenia caryophyllus), peppermint (Mentha piperitae), basil (Ocimum sanctum), rosemary (Rosmarinus officinalis), cinnamon (Cinnamomum zeylanicum) and black cumin (Nigella sativa) were used to identify their antibacterial properties against Yersinia ruckeri, Aeromonas hydrophila, Vibrio anguillarum, Vibrio alginolyticus, Lactococcus garvieae and Vagococcus salmoninarum at five concentrations using disc diffusion method. Especially the EOs of clove, cinnamon and rosemary showed the strongest antibacterial activities than other oils against the three most susceptible bacterial strains (Y. ruckeri, A. hydrophila and V. salmoninarum). Besides, the EOs of clove, rosemary, cinnamon and black cumin showed similar inhibition zones with OTC against A. hydrophila. The minimum inhibitory concentrations of the used EOs found between 500 and 62.5 µl mL-1. As a result, three of the EOs used in this study were effective on both Gr (-) and Gr (+) bacteria.

scholarly journals Antimicrobial Activity of Ethanolic Extract of Medicinal Plants against Human Pathogenic Bacteria

2018 ◽  
Vol 5 ◽  
pp. 1-6
Author(s):  
Hira Sakha ◽  
Rejila Hora ◽  
Shilpa Shrestha ◽  
Shreeya Acharya ◽  
Dinesh Dhakal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Human Pathogenic Bacteria ◽  
Neem Leaves ◽  
Ethanolic Extracts

Objectives: To evaluate the antimicrobial activity of medicinal plants against human pathogenic bacteria and perform Minimum Inhibitory Concentration (MIC) of plants extracts. Methods: Rhizome of Curcuma longa, dried buds of Synzygium aromaticum, seeds of Zanthoxylum armatum and leaves of Elaeocarpus ganitrus, Psidium guajava, Azadirachta indica, and Artemisia vulgaris were collected from hilly regions of Nepal. The plant parts were air-dried at room temperature for several days and grinded to powder form. The ethanolic extracts of medicinal plants were prepared by using the percolation process of extraction using separating funnel and tested against human pathogenic bacteria by disc diffusion method. Then, Minimum Inhibitory Concentration (MIC) of the plant extracts were performed. Results: All plants extracts exhibited antibacterial properties against bacteria under study. However, extract from S. aromaticum (Clove), P. guajava (Guava) and E. ganitrus (Rudraksh) leaves showed most promising result against Staphylococcus aureus with zone of inhibition of 14mm, 16mm and 16 mm respectively. Likewise, S. aromaticum (Clove), C. longa (Turmeric) and P. guajava (Guava) showed good antibacterial activity against Escherichia coli with zone of inhibition of 11mm, 11mm and 10mm respectively. A. vulgaris (Titepati) and A. indica (Neem leaves) showed promising activity against Pseudomonas aeruginosa with zone of inhibition of 11mm. Z. armatum (Timur) showed good result against E. coli with zone of inhibition 10mm. MIC values of ethanolic extracts of S. aromaticum and E. ganitrus were found to be at the range of 12.5-25mg/ml. Conclusion: This study has helped to understand the use of these plants as traditional medicine in an economic and safe alternative to treat infectious diseases.

Sign in / Sign up

Export Citation Format

Share Document