Aromatase Gene Polymorphism in Sheep Breeds of India

Background: Endometriosis implant has been known to express aromatase enzyme, Cytochrome p450 that catalyzes androgen into estrogen. It causes local estrogen production, leading to increased estrogen level, and subsequently triggers endometriosis lesion. CYP19 gene resided at chromosome 15q21.1 is the biggest component of aromatase coding cytochrome p450 enzyme. Objective: To identify relationship between aromatase gene polymorphism CYP19 Val 80 and the risk of endometriosis. Methods: This is an observational case-control study using frozen DNA sample from women with endometriosis and/ or adenomyosis who had undergone laparotomy/ laparoscopy at Obstetrics and Gynecology Department Dr. Mohammad Hoesin General Hospital Palembang January-November 2013. Samples were amplified and cut by PCR-FRLP using Rsa1 restriction enzyme. Results were divided into A/A genotype (homozygote mutant), G/A (heterozygote mutant), and G/G (homozygote wild type). Data were analyzed by SPSS 21.0 version. Results: PCR-RFLP results for A/A genotype were 20 (21.3%) in endometriosis group and 8 (8.5%) in control group. G/A genotype were 18 (19.1%) in endometriosis group and 22 (23.4%) in control group. G/G genotype were 9 (9.6%) and 17 (18.1%) in endometriosis group and control group, respectively. There was significant increase risk of endometriosis in women carrying genotype A/A to those with genotype G/G with OR 4.722 (p<0.05). Conclusion: Polymorphism on aromatase gene CYP19 Val 80 A/A increases risk of endometriosis.

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Identification of CLPG gene polymorphism using PCR-RFLP of Iraq and Belarus population sheep breeds

Gene Reports ◽

10.1016/j.genrep.2020.100974 ◽

2021 ◽

Vol 22 ◽

pp. 100974

Author(s):

ALsaedi Raad Raheem Tolee ◽

Epishko Olga ◽

Cheburanova Ekaterina

Keyword(s):

Gene Polymorphism ◽

Sheep Breeds ◽

Pcr Rflp

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MTNR-1A Gene Polymorphism in Two Tropical Sheep breeds of South India

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.14.2.10 ◽

2018 ◽

Vol 14 (2) ◽

Author(s):

C. R. Vibha ◽

Nagaraja Ramakrishnappa ◽

G. S. Naveen Kumar ◽

R. Jayashree ◽

B. M. Ravindranath

Keyword(s):

Genetic Polymorphism ◽

Gene Polymorphism ◽

South India ◽

Rflp Analysis ◽

Exon 2 ◽

Differential Fertility ◽

Sheep Breeds ◽

Tropical Sheep

The present study was carried out to evaluate the genetic polymorphism in Ovine MTNR1A of two tropical breeds ie., highly prolific NARI Suwarna sheep and Mandya sheep from southern peninsular region of India with respect to differential fertility. Fifty animals from each breed were randomly selected and subjected to RsaI-RFLP analysis of exon-2 of Ovine MTNR1A. The study revealed monomorphic patterns in all the animals for both the breeds. PCR amplicon of 824 bp fragments with four cut sites at 53 bp, 320 bp, 343 bp and 754 bp, resulted in 411 bp, 267 bp, 70 bp, 53 bp and 23 bp. Only presence of CC genotypes at rs406779174 SNP of Ovine MTNR1A in both the breeds supports non-seasonality in breeding of these animals and no association of SNP with increased fecundity of NARI Suwarna sheep over Mandya sheep.

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Aromatase Gene Polymorphism (rs2470152) in Polycystic Ovary Syndrome Patients of Eastern Uttar Pradesh

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2020/44643.14098 ◽

2020 ◽

Author(s):

Kusum Kusum ◽

Sandeep Patel ◽

Radha Chaube ◽

Ashish Ashish ◽

Sangeeta Rai

Keyword(s):

Polycystic Ovary Syndrome ◽

Gene Polymorphism ◽

Diagnostic Criteria ◽

Polycystic Ovary ◽

Uttar Pradesh ◽

Snp Analysis ◽

Genotype Distribution ◽

Ovary Syndrome ◽

Aromatase Gene ◽

Eastern Uttar Pradesh

Introduction: Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder occurring in fertile age women. Its prevalence is increasing and varies day by day in the present population depending on the diagnostic criteria. The diagnostic features of PCOS are known at least but the causative factors which directly or indirectly play a part in it are yet not known. Few studies have demonstrated that aromatase gene with many factors (hormonal, environmental etc.,) may play a role together for the causes of PCOS. Aim: The purpose of this study was to characterise the salient features, frequency of PCOS in Eastern Uttar Pradesh population and aromatase gene polymorphism (rs2470152) analysis with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) as reference gene. Materials and Methods: It was a pilot study where participants were recruited from SS Hospital, BHU between January 2018-2019. Participants completed a questionnaire with proforma and had to undergo physical examination before inclusion in the study. Single Nucleotide Polymorphisms (SNP) analysis was done for aromatase gene by PCR-RFLP. Allele and genotype distribution among groups were evaluated using the chi-square test or Fisher’s-Exact test. Two-tailed p-values less than 0.05 were considered statistically significant.Results: The data indicated that the prevalence of PCOS was high in reproductive age group and hormone profiles differ in different participants, with features of PCOS. With this it was found that aromatase gene was expressed in both control and PCOS patients, but the aromatase gene polymorphism was not significantly related to PCOS. Conclusion: PCOS was increasingly high in reproductive women of eastern Uttar Pradesh population. Its diagnostic criteria have many parameters that differ from individual to individual. The aromatase gene polymorphism is not found significantly associated with PCOS in the Eastern Uttar Pradesh population.

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