Bio-efficacy of Bacillus subtilis in management of meloidogyne incognita infesting tuberose

2018 ◽  
Vol 26 (2) ◽  
pp. 393
Author(s):  
Prasanna Holajjer ◽  
Tarak Nath Saha ◽  
K.S. Girish
Keyword(s):  
Bacillus Subtilis ◽  
Meloidogyne Incognita

EXPRESIÓN DE GENES RELACIONADOS CON PATOGÉNESIS EN CHILE (Capsicum annuum L.) INOCULADO CON Bacillus subtilis Y Meloidogyne enterolobii

Agrociencia ◽  
2021 ◽  
Vol 55 (3) ◽  
pp. 261-272
Author(s):  
Luis Yobani Gayosso Rosales ◽  
Edgar Villar Luna ◽  
María Dolores Rodríguez Torres ◽  
María Valentina Angoa Pérez ◽  
Hortencia Gabriela Mena Violante ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Capsicum Annuum ◽  
Meloidogyne Incognita ◽  
Capsicum Annuum L ◽  
Meloidogyne Enterolobii ◽  
Expresión Génica

El cultivo de chile (Capsicum annuum L.) destaca por el valor agro-alimenticio alto del producto, aunado a su valor comercial. Los nematodos Meloidogyne incognita y M. enterolobii (Me) afectan al cultivo; M. enterolobii (Me) es el más relevante por su agresividad notable. El estudio de alternativas ecológicas es de interés para control estos fitoparásitos. Los objetivos de esta investigación fueron conocer el efecto de Bacillus subtilis (Bs) (CH90) sobre la expresión de los genes PR-1, PR-5, y PR-12 que codifican proteínas relacionadas con patogénesis en chile cv. California Wonder (Cw) infectado con Me; y evaluar el efecto de Bs sobre agallamiento (A) y producción de huevos (H) del nematodo en raíces de Cw. Dos experimentos independientes (E1 y E2) se establecieron con diseño completamente al azar. En E1 y E2 los tratamientos fueron: Cw inoculado solo con Me (CwMe), Cw con Bs y Me (CwBsMe), Cw solo con Bs (CwBs), y Cw sin inoculación (Cw). En ambos experimentos, el nivel de inóculo de Bs fue 108 UFC mL-1, y para Me fue 500 J2 por planta. En E1 la expresión génica se determinó a 3, 7, y 14 d después de inoculación (DPI) con Me. En E2 las variables A y H se evaluaron 45 DPI con Me. PR-1 y PR-5 se sobre expresaron 3 y 7 DPI en los tratamientos CwBsMe y CwBs, en contraste con CwMe (p≤0.05). A los 14 DPI, los genes en todos los tratamientos tuvieron una expresión menor (p≤0.05). La sobre expresión máxima de PR-12 se registró a 14 DPI en los tratamientos CwBsMe y CwBs (p≤0.05). Las plantas de cv. C. Wonder tratadas con B. subtilis CH90 solo o en combinación con M. enterolobii activaron las rutas de defensa dependientes del ácido salicílico (AS) y jasmónico (AJ) pero dicha activación no afectó la reproducción del nematodo en raíces de chile.

scholarly journals Potential of Pseudomonas putida, Bacillus subtilis, and their mixture on the management of Meloidogyne incognita, Pectobacterium betavasculorum, and Rhizoctonia solani disease complex of beetroot (Beta vulgaris L.)

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Manzoor R. Khan ◽  
Zaki A. Siddiqui
Keyword(s):  
Bacillus Subtilis ◽  
Rhizoctonia Solani ◽  
Pseudomonas Putida ◽  
Beta Vulgaris ◽  
Meloidogyne Incognita ◽  
Root Rot ◽  
Growth Parameters ◽  
Principal Component ◽  
Soft Rot ◽  
Disease Complex

AbstractEffects of Pseudomonas putida and Bacillus subtilis alone, and in combinations for the management of Meloidogyne incognita, Pectobacterium betavasculorum, and Rhizoctonia solani disease complex of beetroot (Beta vulgaris L.), were studied. Application of P. putida or B. subtilis to plants with M. incognita or P. betavasculorum or R. solani singly or in combinations caused a significant increase in plant growth parameters and the activities of defense enzymes. A significant increase in chlorophyll fluorescence attributes, viz., Fv/Fm, ɸPSII, qP, NPQ, and ETR were recorded in plants treated with P. putida or B. subtilis over pathogen-inoculated plants. Inoculation of P. putida results in a higher reduction in galling and nematode multiplication than B. subtilis. Maximum reduction in nematode multiplication and galling occurred when a mixture of P. putida and B. subtilis was used. Soft rot and root rot indices were 3 when Pectobacterium betavasculorum and Rhizoctonia solani were inoculated alone. The disease indices were rated 5 when these pathogens and M. incognita were inoculated in combinations. Inoculation of P. putida/B. subtilis with P. betavasculorum or R. solani reduced soft rot and root rot indices to 2 out of 3, while the use of P. putida + B. subtilis reduced indices to 1. Disease indices were reduced to 2–3 out of 5, when P. putida + B. subtilis were used to plants inoculated with two or three pathogens. The principal component analysis showed significant correlations among the various studied attributes. Two principal components explained a total of 86.1 and 93.4% of the overall data variability. Therefore, the use of P. putida together with B. subtilis had the potential for successful management of disease complex of beetroot.

scholarly journals Bioefficacy of Bacillus subtilis against root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood in tomato

2015 ◽  
Vol 7 (2) ◽  
pp. 1012-1015
Author(s):  
Subhalaxmi Roy ◽  
Arun Rathod ◽  
Aniruddha Pramanik
Keyword(s):  
Bacillus Subtilis ◽  
Trichoderma Harzianum ◽  
Meloidogyne Incognita ◽  
Dry Weight ◽  
Control Agent ◽  
Seedling Height ◽  
Root Knot Nematode ◽  
Tomato Fruits ◽  
Highest Weight ◽  
Gall Index

An investigation was conducted for the management of root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood infesting tomato through the application of bio-control agent like Bacillus subtilis, Trichoderma harzianum and Pseudomonas fluorescens. Experiment result revealed that minimum no. of galls/25seedlings (17.50) and maximum seedling height (27.6cm) were observed in Bacillus subtilis @50g/m2 in nursery bed + B. subtilis @ 5kg along with 2.5 tons of FYM/ ha. The highest weight/25seedlings (69.50g) was noticed in the B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha. The highest growth of the plant at 45 DAT (49.2cm) and at harvest (81.2cm) and maximum fresh (711.3g) and dry weight (265g) was found in B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha. B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha exhibited lowest gall index (1.2/plant) and highest reduction of nematode population and provided highest yield of tomato fruits (335.75q/ha).

scholarly journals Polymer-based encapsulation of Bacillus subtilis and its effect on Meloidogyne incognita in tomato

Phyton ◽  
2016 ◽  
Vol 85 (1) ◽  
pp. 1-6
Author(s):  
Pacheco-Aguirre J ◽  
E Ruiz-S醤chez ◽  
A Reyes-Ram韗ez ◽  
J Crist骲al-Alejo ◽  
J Tun-Su醨ez ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Meloidogyne Incognita

scholarly journals Pengaruh PGPR terhadap Penekanan Populasi Nematoda Puru Akar (Meloidogyne incognita (Kofoid and White) Chitwood) pada Tanaman Kenaf (Hibiscus cannabinus L.)

2016 ◽  
Vol 8 (1) ◽  
pp. 30
Author(s):  
Kristiana Sri Wijayanti ◽  
Bambang Tri Rahardjo ◽  
Toto Himawan
Keyword(s):  
Bacillus Subtilis ◽  
Meloidogyne Incognita ◽  
Block Design ◽  
Plant Growth Promoting Rhizobacteria ◽  
Hibiscus Cannabinus ◽  
Control Treatment ◽  
Randomized Block Design ◽  
Two Factors ◽  
Plant Growth Promoting ◽  
Nematode Eggs

<p>Tanaman kenaf yang terinfeksi nematoda <em>Meloidogyne incognita</em><em> </em><em> </em>dapat mempengaruhi pertumbuhan tanaman dan produksi serat. Penelitian ini bertujuan untuk mengevaluasi pengaruh <em>Plant Growth Promoting Rhizobacteria </em>(PGPR) dalam menekan populasi nematoda <em>M. incognita</em> pada tanaman kenaf di rumah kaca. Penelitian dirancang dengan menggunakan rancangan acak kelompok (RAK) faktorial dengan dua faktor, faktor pertama adalah cara aplikasi PGPR yang terdiri atas 2 cara yaitu suspensi PGPR diberikan sebelum tanam dengan merendam benih selama 5 jam (C1), benih ditanam langsung dalam pot tanpa direndam dalam PGPR (C2), dan suspensi PGPR diberikan pada 15 hari setelah tanam (HST) dan 25 HST. Faktor kedua adalah jenis PGPR yang digunakan yaitu <em>P</em><em>seudomonas</em><em> fluorescens</em>, <em>Bacillus subtilis, Azotobacter</em> sp., <em>P</em><em>. </em><em>fluorescen</em><em>s </em>+ <em>B. subtilis, </em><em>P</em><em>. </em><em>fluorescen</em><em>s </em>+ <em>Azotobacter</em> sp., <em>B. subtilis </em>+ <em>Azotobacter</em> sp., dan <em>P</em><em>. </em><em>fluorescen</em><em>s </em>+ <em>B. subtilis </em>+ <em>Azotobacter</em> sp., serta kontrol (tanpa PGPR).  Hasil penelitian menunjukkan bahwa perendaman benih dengan kombinasi tiga bakteri memberikan pengaruh yang nyata terhadap populasi juvenil nematoda dalam tanah, sedangkan perlakuan tanpa perendaman tidak memberikan pengaruh. Populasi juvenil nematoda di dalam akar yang diberi PGPR baik tunggal maupun kombinasi melalui perendaman benih atau tanpa perendaman benih tidak berpengaruh, kecuali pada kombinasi <em>P</em><em>.</em><em> fluorescens </em>dan <em>B. subtilis</em><em> </em>yang diberikan melalui perendaman benih mampu menekan populasi juvenil nematoda di akar 43,28% bila dibandingkan tanpa perendaman benih. Pemberian rizobakteri <em>P</em><em>.</em><em> fluorescens</em>  menurunkan jumlah telur nematoda terbanyak (86,39%) dan menekan intensitas penyakit sebesar 71,95% bila dibandingkan kontrol.</p><p><strong></strong>Infection of <em>Meloidogyne incognita</em> on kenaf could affect its growth and the production of fiber. This study aimed to evaluate the effect of PGPR on the reduction of nematode <em>M. incognita</em> population on kenaf in the greenhouse. The factorial experiment was laid on randomized block design. The study consisted of two factors with three replicates . The first factor was method of PGPR application, ie: PGPR suspension was given before planting (kenaf seeds was soaked for 5 hours) (C1) and the seeds directly planted without submerged (C2), PGPR suspension was given at 15 days after planting (DAP) and 25 dap. The second factor was type of bacteria (<em>Pseudomonas  fluorescens</em>,  <em>Bacillus  subtilis,  Azotobacter</em>  sp.,  <em>P.  fluorescens </em>+ <em>B. subtilis, </em><em>P. fluorescens </em>+ <em>Azotobacter</em>  sp., <em>B. subtilis </em>+ <em>Azotobacter</em>  sp.,  and  <em>P.  fluorescens  </em>+  <em>B. subtilis   </em>+ <em>Azotobacter</em>  sp.) and control. The results showed that submerged seed with the three bacterial rhizobacteria significant compared to the control treatment and single treatment and two combination rhizobacteria, while without submerged seed with single or combination rhizobacteria not significant on the population of juvenile nematodes in the soil. Combination of <em>P. fluorescens </em>and<em> B. subtilis</em>with submerged seed capable of suppressing the population of  juvenile  nematodes  in the  roots  of 43.28%  when  compared with or without submerged seed.   Population  of  juvenile  nematodes  in the  roots by submerged seed and without submerged seed either single or combination rhizobacteria do not affect each other. <em>P. fluorescens </em>suppress nematode eggs are highest 86.39% and disease intensity by 71,95% where compared to control.</p>

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