Bio-nematicidal activities by culture filtrate of Bacillus subtilis HussainT-AMU: new promising biosurfactant bioagent for the management of Root Galling caused by Meloidogyne incognita

2020 ◽  
Vol 33 (2) ◽  
pp. 229-238 ◽  
Author(s):  
Touseef Hussain ◽  
Mohammad Haris ◽  
Adnan Shakeel ◽  
Gufran Ahmad ◽  
Abrar Ahmad Khan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Culture Filtrate ◽  
Meloidogyne Incognita

scholarly journals Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21

2022 ◽  
Vol 8 (1) ◽  
pp. 80
Author(s):  
Yongmei Li ◽  
Mengyuan Xia ◽  
Pengbo He ◽  
Qiaoming Yang ◽  
Yixin Wu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Culture Filtrate ◽  
Management Strategies ◽  
Citrus Fruit ◽  
Inhibition Zone ◽  
Penicillium Digitatum ◽  
Antifungal Compounds ◽  
Fruit Peel ◽  
Post Harvest

Citrus is among the most important plants in the fruit industry severely infected with pathogens. Citrus green mold caused by Penicillium digitatum is one of the most devastating diseases during post-harvest stages of citrus fruit. In this study, a potential endophyte Bacillus subtilis L1-21, isolated from healthy citrus plants, was assessed for its biocontrol activity against the pathogen P. digitatum. Based on an in vitro crosstalk assay, we suggested that B. subtilis L1-21 inhibits the pathogen with an inhibition zone of 3.51 ± 0.08 cm. Biocontrol efficacy was highest for the fermented culture filtrate of B. subtilis L1-21. Additionally, using GC-MS analysis, 13 compounds were detected in the extract of this endophyte. The culture filtrate in Landy medium could enlarge and deform pathogen spores and prevent them from developing into normal mycelium. Accordingly, the Landy culture filtrate of B. subtilis L1-21 was stable in the temperature range of 4–90 °C and pH of 3–11. Further, MALDI-TOF-MS for B. subtilis L1-21 detected surfactin, fengycin, bacillaene and bacilysin as potential antifungal compounds. GFP-tagged B. subtilis L1-21 easily colonized in citrus fruit peel and pulp, suggesting its role in eliminating the fungal pathogen. Altogether, it is highly expected that the production of antifungal compounds, and the colonization potential of B. subtilis L1-21 are required against the post-harvest P. digitatum pathogen on citrus fruit.

EXPRESIÓN DE GENES RELACIONADOS CON PATOGÉNESIS EN CHILE (Capsicum annuum L.) INOCULADO CON Bacillus subtilis Y Meloidogyne enterolobii

Agrociencia ◽  
2021 ◽  
Vol 55 (3) ◽  
pp. 261-272
Author(s):  
Luis Yobani Gayosso Rosales ◽  
Edgar Villar Luna ◽  
María Dolores Rodríguez Torres ◽  
María Valentina Angoa Pérez ◽  
Hortencia Gabriela Mena Violante ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Capsicum Annuum ◽  
Meloidogyne Incognita ◽  
Capsicum Annuum L ◽  
Meloidogyne Enterolobii ◽  
Expresión Génica

El cultivo de chile (Capsicum annuum L.) destaca por el valor agro-alimenticio alto del producto, aunado a su valor comercial. Los nematodos Meloidogyne incognita y M. enterolobii (Me) afectan al cultivo; M. enterolobii (Me) es el más relevante por su agresividad notable. El estudio de alternativas ecológicas es de interés para control estos fitoparásitos. Los objetivos de esta investigación fueron conocer el efecto de Bacillus subtilis (Bs) (CH90) sobre la expresión de los genes PR-1, PR-5, y PR-12 que codifican proteínas relacionadas con patogénesis en chile cv. California Wonder (Cw) infectado con Me; y evaluar el efecto de Bs sobre agallamiento (A) y producción de huevos (H) del nematodo en raíces de Cw. Dos experimentos independientes (E1 y E2) se establecieron con diseño completamente al azar. En E1 y E2 los tratamientos fueron: Cw inoculado solo con Me (CwMe), Cw con Bs y Me (CwBsMe), Cw solo con Bs (CwBs), y Cw sin inoculación (Cw). En ambos experimentos, el nivel de inóculo de Bs fue 108 UFC mL-1, y para Me fue 500 J2 por planta. En E1 la expresión génica se determinó a 3, 7, y 14 d después de inoculación (DPI) con Me. En E2 las variables A y H se evaluaron 45 DPI con Me. PR-1 y PR-5 se sobre expresaron 3 y 7 DPI en los tratamientos CwBsMe y CwBs, en contraste con CwMe (p≤0.05). A los 14 DPI, los genes en todos los tratamientos tuvieron una expresión menor (p≤0.05). La sobre expresión máxima de PR-12 se registró a 14 DPI en los tratamientos CwBsMe y CwBs (p≤0.05). Las plantas de cv. C. Wonder tratadas con B. subtilis CH90 solo o en combinación con M. enterolobii activaron las rutas de defensa dependientes del ácido salicílico (AS) y jasmónico (AJ) pero dicha activación no afectó la reproducción del nematodo en raíces de chile.

scholarly journals Potential of Pseudomonas putida, Bacillus subtilis, and their mixture on the management of Meloidogyne incognita, Pectobacterium betavasculorum, and Rhizoctonia solani disease complex of beetroot (Beta vulgaris L.)

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Manzoor R. Khan ◽  
Zaki A. Siddiqui
Keyword(s):  
Bacillus Subtilis ◽  
Rhizoctonia Solani ◽  
Pseudomonas Putida ◽  
Beta Vulgaris ◽  
Meloidogyne Incognita ◽  
Root Rot ◽  
Growth Parameters ◽  
Principal Component ◽  
Soft Rot ◽  
Disease Complex

AbstractEffects of Pseudomonas putida and Bacillus subtilis alone, and in combinations for the management of Meloidogyne incognita, Pectobacterium betavasculorum, and Rhizoctonia solani disease complex of beetroot (Beta vulgaris L.), were studied. Application of P. putida or B. subtilis to plants with M. incognita or P. betavasculorum or R. solani singly or in combinations caused a significant increase in plant growth parameters and the activities of defense enzymes. A significant increase in chlorophyll fluorescence attributes, viz., Fv/Fm, ɸPSII, qP, NPQ, and ETR were recorded in plants treated with P. putida or B. subtilis over pathogen-inoculated plants. Inoculation of P. putida results in a higher reduction in galling and nematode multiplication than B. subtilis. Maximum reduction in nematode multiplication and galling occurred when a mixture of P. putida and B. subtilis was used. Soft rot and root rot indices were 3 when Pectobacterium betavasculorum and Rhizoctonia solani were inoculated alone. The disease indices were rated 5 when these pathogens and M. incognita were inoculated in combinations. Inoculation of P. putida/B. subtilis with P. betavasculorum or R. solani reduced soft rot and root rot indices to 2 out of 3, while the use of P. putida + B. subtilis reduced indices to 1. Disease indices were reduced to 2–3 out of 5, when P. putida + B. subtilis were used to plants inoculated with two or three pathogens. The principal component analysis showed significant correlations among the various studied attributes. Two principal components explained a total of 86.1 and 93.4% of the overall data variability. Therefore, the use of P. putida together with B. subtilis had the potential for successful management of disease complex of beetroot.

Characterization of an antifungal soil bacterium and its antagonistic activities againstFusariumspecies

2003 ◽  
Vol 49 (4) ◽  
pp. 253-262 ◽  
Author(s):  
Yiu-Kwok Chan ◽  
Wayne A McCormick ◽  
Keith A Seifert
Keyword(s):  
Bacillus Subtilis ◽  
Culture Filtrate ◽  
Fusarium Species ◽  
Hyphal Growth ◽  
Antagonistic Activity ◽  
Plant Diseases ◽  
Ear Rot ◽  
In Planta ◽  
Head Blight

Bacteria were isolated from a cultivated soil and screened for antagonistic activity against Fusarium graminearum, a predominant agent of ear rot and head blight in cereal crops. Based on its in vitro effectiveness, isolate D1/2 was selected for characterization and identified as a strain of Bacillus subtilis by phenotypic tests and comparative analysis of its 16S ribosomal RNA gene (rDNA) sequence. It inhibited the mycelial growth of a collection of common fungal phytopathogens, including eight Fusarium species, three other ascomycetes, and one basidiomycete. The cell-free culture filtrate of D1/2 at different dilutions was active against macroconidium germination and hyphal growth of F. graminearum, depending on the initial macroconidium density. It induced the formation of swollen hyphal cells in liquid cultures of this fungus grown from macroconidia. A bioassay also demonstrated that D1/2 offered in planta protection against the damping-off disease in alfalfa seedlings caused by F. graminearum, while the type strain of B. subtilis was ineffective. Hence, B. subtilis D1/2 or its culture filtrate has potential application in controlling plant diseases caused by Fusarium.Key words: antifungal activity, Bacillus subtilis, biological control, biopesticide, Fusarium species.

scholarly journals Purification and partial characterization of the gastric ulcer inhibitory substance from culture filtrate of Bacillus subtilis H.

1977 ◽  
Vol 25 (10) ◽  
pp. 2770-2774 ◽  
Author(s):  
TSUTOMU MIMURA ◽  
NORIO MUTO ◽  
HIROSHI TSUJIBO ◽  
NOBORU ONISHI ◽  
SHIGERU AONUMA
Keyword(s):  
Bacillus Subtilis ◽  
Gastric Ulcer ◽  
Culture Filtrate ◽  
Partial Characterization ◽  
Inhibitory Substance

scholarly journals Bioefficacy of Bacillus subtilis against root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood in tomato

2015 ◽  
Vol 7 (2) ◽  
pp. 1012-1015
Author(s):  
Subhalaxmi Roy ◽  
Arun Rathod ◽  
Aniruddha Pramanik
Keyword(s):  
Bacillus Subtilis ◽  
Trichoderma Harzianum ◽  
Meloidogyne Incognita ◽  
Dry Weight ◽  
Control Agent ◽  
Seedling Height ◽  
Root Knot Nematode ◽  
Tomato Fruits ◽  
Highest Weight ◽  
Gall Index

An investigation was conducted for the management of root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood infesting tomato through the application of bio-control agent like Bacillus subtilis, Trichoderma harzianum and Pseudomonas fluorescens. Experiment result revealed that minimum no. of galls/25seedlings (17.50) and maximum seedling height (27.6cm) were observed in Bacillus subtilis @50g/m2 in nursery bed + B. subtilis @ 5kg along with 2.5 tons of FYM/ ha. The highest weight/25seedlings (69.50g) was noticed in the B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha. The highest growth of the plant at 45 DAT (49.2cm) and at harvest (81.2cm) and maximum fresh (711.3g) and dry weight (265g) was found in B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha. B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha exhibited lowest gall index (1.2/plant) and highest reduction of nematode population and provided highest yield of tomato fruits (335.75q/ha).

Sign in / Sign up

Export Citation Format

Share Document