Genetic diversity of rice leaf folder using mitochondrial cytochrome oxidase 1 gene

2019 ◽  
Vol 81 (1) ◽  
pp. 75
Author(s):  
Vikas Jindal
2019 ◽  
Vol 23 (2) ◽  
pp. 234
Author(s):  
Ahmad Taufiq Arminudin ◽  
Y. Andi Trisyono ◽  
Arman Wijonarko ◽  
Suputa Suputa

Primer plays an important role in studying genetic diversity of an insect species. This research was aimed to select the suitable primers to visualize the genetic diversity of Asian corn borer (Ostrinia furnacalis) using Random Amplified Polymorphic DNA (RAPD), microsatellite, and mitochondrial cytochrome oxidase subunit 1 gene (mtCO1). Twenty four RAPD primers (OPA1, OPA4, OPA7,OPA8, OPA10, OPA11, OPA12, OPA13, OPB7, OPB10, OPB11, OPB12, OPB15, OPC4, OPC5, OPC14, OPC16, OPC18, OPC20, OPD3, OPD8, OPD10, OPD13, OPD14) and five microsatellite primers (T3, T4, T5, T81, D25) resulted high polymorphic informations of the genetics of O. furnacalis in Java Indonesia. Universal primers, Lep and Heb were appropriateto do molecular identification of O. furnacalis based on BLAST system on GenBank and BOLD systems.


2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Vista Budiariati ◽  
Trini Susmiati ◽  
Siti Munawaroh ◽  
Rachmawati Cahyaningtyas Arie Putri ◽  
Rini Widayanti Widayanti

Abstract. Budiariati V, Susmiati T, Waroh S, Putri RCA, Widayanti R. 2021. Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene. Biodiversitas 22: 593-600. Catfish is one of the most demanding fish in Indonesia and served in a variety of traditional culinary. Due to their identical morphology and close relation between species in the order of Siluriformes, it is quite tricky to distinguish the species. This can be a threat to develop catfish production in Indonesia since there is a wide variety of catfish species in this mega biodiversity country. The study aimed to analyze the genetic diversity of Indonesian indigenous catfish especially those known as Baung fish by local people based on COII gene. The study also aimed to determine the phylogenetic relationship between the samples and compare them with the GenBank data. A total of 24 samples used in this study from 8 different rivers from 3 different islands and two samples were collected from coastal areas. The study results showed that there is genetic diversity of the Indonesian indigenous catfish based on COII gene. The sequences among 24 samples showed that from 691 nucleotides of COII gene, there were very subtle nucleotides differences of samples that originated from Bojonegoro, Magelang, and samples collected from Baru Beach, Yogyakarta. Based on COII amino acid sequences, 6 polymorphic amino acid sites were on-site number 64, 115, 123, 129, 144, and 165. The samples encoded LLB1 and LPB1 from Baru Beach, Yogyakarta, showed highest different amino acids in the six sites. Samples from the river of Central Java, Sumatra, and Kalimantan belonged to Bagridae family and consist of two different species Hemibagrus sp. and Mystus sp while samples from East Java belonged to Pangasiidae family. The Samples from coastal belonged to Ariidae family.


Author(s):  
Jean Raleigh ◽  
Niamh E. Redmond ◽  
Emma Delahan ◽  
Seamus Torpey ◽  
Rob W.M. van Soest ◽  
...  

Recent molecular studies have shown that the sponge order Haploslcerida is polyphyletic as the freshwater sponges appear to be more closely related to other demosponges than they are to the marine haplosclerids. Within the marine haplosclerid clade relationships viewed via 18S and 28S rRNA gene phylogenies suggest that the suborders and many families and genera are also polyphyletic. However, both of these genes are on the same locus and do not evolve completely independently. We have analysed mitochondrial Cytochrome oxidase 1 gene fragments from 44 species of marine Haplosclerida and show conclusively that the classification of this group needs complete revision. Molecular data show a very complicated phylogeny supporting very few morphological hypotheses and little geographical pattern. However, the molecular data contain a great deal of phylogenetic signal at many taxonomic levels and support phylogenies drawn from the other genes.


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