Character Association and Genetic Diversity in Rainfed Greengram [Vigna radiata (L.) Wilczek]

Mung bean [Vigna radiata (L.) Wilczek] is an important short-duration grain legume widely known for its nutritional, soil ameliorative, and cropping system intensification properties. This study aims at evaluating genetic diversity among mung bean genotypes and detecting genomic regions associated with various yield attributing traits and yellow mosaic disease (YMD) resistance by association mapping. A panel of 80 cultivars and advanced breeding lines was evaluated for 10 yield-related and YMD resistance traits during kharif (monsoon) and summer seasons of 2018–2019 and 2019–2020. A total of 164 genome-wide simple sequence repeat (SSR) markers were initially screened, out of which 89 were found polymorphic which generated 317 polymorphic alleles with an average of 3.56 alleles per SSR locus. The number of alleles at each locus varied from 2 to 7. The population genetic structure analysis grouped different genotypes in three major clusters and three genetically distinct subpopulations (SPs) (i.e., SP-1, SP-2, and SP-3) with one admixture subpopulation (SP-4). Both cluster and population genetic structure analysis categorized the advanced mung bean genotypes in a single group/SP and the released varieties in other groups/SPs, suggesting that the studied genotypes may have common ancestral history at some level. The population genetic structure was also in agreement with the genetic diversity analysis. The estimate of the average degree of linkage disequilibrium (LD) present at the genome level in 80 mung bean genotypes unveiled significant LD blocks. Over the four seasons, 10 marker-trait associations were observed significant for YMD and four seed yield (SY)-related traits viz., days to flowering, days to maturity, plant height, and number of pods per plant using the mixed linear model (MLM) method. These associations may be useful for marker-assisted mung bean yield improvement programs and YMD resistance.

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Agromorphological Evaluation of 44 lines of Mung Bean (Vigna radiata (L.) Wilczek) Introduced in Burkina Faso

European Scientific Journal ESJ ◽

10.19044/esj.2021.v17n40p20 ◽

2021 ◽

Vol 17 (40) ◽

pp. 20

Author(s):

Wend-Pagnagde Félicien Marie Serge Zida ◽

Teyoure Benoit Joseph Batieno ◽

Tinga Jeremy Ouedraogo ◽

Mahamadou Sawadogo

Keyword(s):

Genetic Diversity ◽

Burkina Faso ◽

Coefficient Of Variation ◽

Vigna Radiata ◽

Mung Bean ◽

Coat Color ◽

Qualitative Traits ◽

High Heritability ◽

Important Basis ◽

Cowpea Variety

Mung bean (Vigna radiata L. Wilczek) is an important annual legume which is well suited to dry areas, like Burkina Faso where it is still an orphan legume. The present study was conducted to evaluate 44 mung bean genotypes introduced in Burkina Faso and to determine genetic diversity in this collection using both quantitative and qualitative traits. A field experiment was conducted in an augmented bloc design with three blocks and an extraearly maturing cowpea variety as check. Data were recorded on six (06) quantitative traits and seven (07) qualitative traits. For qualitative traits, diversity was revealed in four parameters including hypocotyl’s color, seed coat color, pods and stem pubescence. Significant genetic variability was revealed among genotypes for all the quantitative characters. Phenotypic coefficient of variation (PCV) was higher than Genotypic coefficient of variation (GCV) for all characters studied indicating the influence of environmental effect on the characters. The GCV and PCV estimates were high for number of pods per plant followed by plant height and hundred seeds weigh. The high heritability coupled with high genetic advanced as percent of mean observed for these traits suggest an important genetic gain in genetic improvement for these characters. The genetic diversity observed was grouped into five clusters. Mungbean lines were grouped into four clusters and the cowpea variety used as check crop in this trial was clustered apart. This genetic diversity, both qualitatively and quantitatively, will help enrich local biodiversity in terms of legumes in general and mung bean in particular and constitutes an important basis for a future mungbean breeding program.

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Utilization of genetic diversity and its association characters in mungbean [Vigna radiata(L.) Wilczek]

Legume Research - An International Journal ◽

10.5958/0976-0571.2014.00696.1 ◽

2014 ◽

Vol 37 (6) ◽

pp. 679

Author(s):

Mohan Lal ◽

Dhirendra Singh

Keyword(s):

Genetic Diversity ◽

Vigna Radiata

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Genetic diversity of mungbean (Vigna radiata L.) germplasm in Indonesia

Plant Genetic Resources ◽

10.1017/s1479262114000343 ◽

2014 ◽

Vol 12 (S1) ◽

pp. S91-S94 ◽

Cited By ~ 7

Author(s):

Puji Lestari ◽

Sue Kyung Kim ◽

Reflinur ◽

Yang Jae Kang ◽

Nurwita Dewi ◽

...

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Vigna Radiata ◽

Diversity Index ◽

Polymorphic Information Content ◽

Conservation Strategy ◽

Low Genetic Diversity ◽

Breeding Programmes ◽

The Mean ◽

Simple Sequence

Despite widespread mungbean [Vigna radiata (L.) Wilczek] consumption in Indonesia, few molecular studies have been carried out on accessions and available data are minimal. In this study, we used 30 newly developed simple sequence repeat (SSR) markers designed from the mapped sequence scaffolds of the Korean Sunhwanokdu and Gyeonggijaerae 5 mungbean genomes. These markers were used to examine loci in 83 mungbean accessions collected from diverse geographical areas in Indonesia. A total of 107 alleles were detected among the accessions with 29 polymorphic markers. However, the mean of polymorphic information content (0.33) value and diversity index (0.38) value was indicative of low genetic diversity in this germplasm. The mungbean population structure was not clearly differentiated and the number of subpopulations was unclear. Neighbour-joining tree analysis revealed that the genetic cluster did not reflect the geographical origin of the accessions. Interestingly, the most agriculturally improved varieties were genetically similar to some landraces from one of the main mungbean-producing regions. These newly developed SSR markers could be useful for detecting genetic variability as a basis for establishing a conservation strategy for mungbean germplasm with the aim of enhancing Indonesian breeding programmes.

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