In vitro evaluation of potential fungal and bacterial isolates against Rhizoctonia solani causing sheath blight of rice

Crop Research ◽  
2018 ◽  
Vol 53 (1and2) ◽  
pp. 63
Author(s):  
Bhavya Mishra ◽  
Roopali Sharma ◽  
B. C. Kabdwal ◽  
Archana Negi ◽  
Anshul Arya
Keyword(s):  
Rhizoctonia Solani ◽  
Sheath Blight ◽  
Bacterial Isolates ◽  
In Vitro Evaluation

scholarly journals In Vitro Efficacy of Five Biofungicides against Rhizoctonia solani Kuhn in Rice

2020 ◽  
Author(s):  
Irish Mae Bauzon-Cantila ◽  
Jaime C. Silvestre ◽  
Raquel B. Evangelista ◽  
Edralyn Catubay
Keyword(s):  
Rhizoctonia Solani ◽  
Aloe Vera ◽  
Fungal Disease ◽  
Sheath Blight ◽  
Low Risk ◽  
High Yield ◽  
Melaleuca Alternifolia ◽  
Causal Pathogen ◽  
Chemical Fungicides

ABSTRACTRhizoctonia solani Kuhn, the causal pathogen of sheath blight is second most damaging fungal disease in rice. While using chemical fungicides present high detriment to environment, the study investigate the efficacy of treatments composing five biofungicides in three different rates along with a biological agent, chemical check and untreated against the pathogen in in vitro level. In vitro efficacy showed that Melaleuca alternifolia + terpenes at 3.00 ml/L of H20 (T6), Aloe vera powder (Manopol) + Melaleuca oil at 3.00 ml/L of H20 (T15) and at 2.00 ml/L of H20 (T14) and Melaleuca alternifolia + terpenes at 2.00 ml/L of H20 (T5) as very effective (0-10 mm diameter zone of growth) treatments comparable to the chemical check (T17). Therefore, attaining high yield rice while having low risk to environment can always be done.

scholarly journals Uji Efektivitas Rizobakteria Dalam Menghambat Perkembangan Penyakit Hawar Pelepah Daun (Rhizoctonia solani Kuhn.) Pada Padi Secara In Vitro

2020 ◽  
Vol 16 (1) ◽  
pp. 44
Author(s):  
Hanisa Desy Ariani ◽  
Noor Aidawati ◽  
Dewi Arika Adriani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Rhizoctonia Solani ◽  
Plant Protection ◽  
Block Design ◽  
Sheath Blight ◽  
In Vitro Test ◽  
Sheath Blight Disease ◽  
Randomized Block Design ◽  
Blight Disease

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.

scholarly journals Isolation and in vitro evaluation of bacteriophages against MDR-bacterial isolates from septic wound infections

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0179245 ◽  
Author(s):  
Roja Rani Pallavali ◽  
Vijaya Lakshmi Degati ◽  
Dakshayani Lomada ◽  
Madhava C. Reddy ◽  
Vijaya Raghava Prasad Durbaka
Keyword(s):  
Wound Infections ◽  
Bacterial Isolates ◽  
In Vitro Evaluation ◽  
Septic Wound

scholarly journals Biological Control of Sheath Blight Disease of Rice by using Antagonistic Bacteria

1970 ◽  
Vol 45 (3) ◽  
pp. 225-232 ◽  
Author(s):  
MA Bashar ◽  
MA Hossain ◽  
MM Rahman ◽  
MN Uddin ◽  
MN Begum
Keyword(s):  
Biological Control ◽  
Rhizoctonia Solani ◽  
Inhibition Zone ◽  
Sheath Blight ◽  
Bacterial Suspension ◽  
Antagonistic Bacteria ◽  
Causal Organism ◽  
Sheath Blight Disease ◽  
Blight Disease

The study was made to detect and identify antagonistic bacteria to control Rhizoctonia solani, a causal organism of sheath blight (ShB) disease of rice. Isolation of antagonistic bacteria was done from ShB infected rice leaf collected from the districts of Gazipur, Rajshahi, Bogra and Comilla. Fifty isolates of bacteria were isolated. These isolates were tested for antagonism against ShB pathogen of in PDA medium. Among the isolates of antagonistic bacteria (both fluorescent and non fluorescent), eleven produced more than 15 mm inhibition zone, were selected for this study. Remarkable inhibition zone producing ten isolates were selected to observe their antagonistic behaviour by soaking the sclerotia of Rhizoctonia solani and rice seedlings in different hours into bacterial suspension of 3.84 x 107 CFU/ml. Both the in vitro and in vivo rom showed that the sclerotial germination and sheath blight symptom expression were delayed 6-9 days. Soaking of both seedlings and sclerotia with the test bacteria was found most effective to control ShB (38% - 100%) than soaking of seedlings alone with bacterial suspension at different hours. However, BanShbFPS5 (2)B, BanShb738(3), BanShb738(2) and BanShb581(1), the four antagonistic bacterial isolates could be applied as biological agent to control sheath blight disease of rice and they could control sheath blight disease development and could delay the epidemics of the disease. Key words: Biological control; Sheath blight disease; Rhizoctonia solani; Antagonistic bacteria DOI: 10.3329/bjsir.v45i3.6529Bangladesh J. Sci. Ind. Res. 45(3), 225-232, 2010

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